Determination of serum creatinine by Jaffe method and how to calibrate to eliminate matrix interference problems

Chromý, V.; Rozkošná, Kateřina; Sedlák, Pavel

doi:10.1515/cclm.2008.224

Cited by 53 publications

(28 citation statements)

References 9 publications

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“…Blood urea nitrogen (BUN) was measured indirectly via the enzymatic method described by Talke and Schubert (22). Creatinine was measured directly via the Jaffe reaction (1).…”

Section: Methodsmentioning

confidence: 99%

Bone marrow mononuclear cells induce beneficial remodeling and reduce diastolic dysfunction in the left ventricle of hypertensive SS/MCWi rats

Parker

Didier

Karcher

et al. 2012

Physiological Genomics

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Little is known about the effectiveness of BMMNC therapy in hypertensive heart disease. In the current study, we show that delivery of BMMNCs from hypertension protected SS-13 BN /MCWi donor rats, but not BMMNC from hypertension susceptible SS/MCWi donor rats, resulted in 57.2 and 83.4% reductions in perivascular and interstitial fibrosis, respectively, as well as a 60% increase in capillary-to-myocyte count in the left ventricles (LV) of hypertensive SS/MCWi recipients. These histological changes were associated with improvements in LV compliance and relaxation (103 and 46.4% improvements, respectively). Furthermore, improved diastolic function in hypertensive SS/MCWi rats receiving SS-13 BN /MCWi derived BMMNCs was associated with lower clinical indicators of heart failure, including reductions in end diastolic pressure (65%) and serum brain natriuretic peptide levels (49.9%) with no improvements observed in rats receiving SS/MCWi BMMNCs. SS/MCWi rats had a lower percentage of endothelial progenitor cells in their bone marrow relative to SS-13 BN /MCWi rats. These results suggest that administration of BMMNCs can prevent or reverse pathological remodeling in hypertensive heart disease, which contributes to ameliorating diastolic dysfunction and associated symptomology. Furthermore, the health and hypertension susceptibility of the BMMNC donor are important factors influencing therapeutic efficacy, possibly via differences in the cellular composition of bone marrow.

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“…Blood urea nitrogen (BUN) was measured indirectly via the enzymatic method described by Talke and Schubert (22). Creatinine was measured directly via the Jaffe reaction (1).…”

Section: Methodsmentioning

confidence: 99%

Bone marrow mononuclear cells induce beneficial remodeling and reduce diastolic dysfunction in the left ventricle of hypertensive SS/MCWi rats

Parker

Didier

Karcher

et al. 2012

Physiological Genomics

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“…Lipids were eluted with 3 ml methanol, solvent was evaporated, and samples were dissolved in methanol for analysis by HPLC-electrospray ionization mass spectrometry (ESI-MS/MS). Nitro-fatty acid levels were normalized to urine creatinine concentrations that were determined using a colorimetric assay measuring absorbance at 535 nm after dilution in NaOH and reaction with picric acid ( 20 ).…”

Section: Cla Nitration Products In Human Urinementioning

confidence: 99%

Characterization and quantification of endogenous fatty acid nitroalkene metabolites in human urine

Salvatore

Vitturi

Baker

et al. 2013

Journal of Lipid Research

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This article is available online at http://www.jlr.org biomolecule nitration ( 1 ). Nitroalkene substituents are electrophilic and promote Michael addition of fatty acids with biological nucleophiles such as cysteine and histidine. The extent, rate, and reversibility of these reactions will be dictated both by the concentration and reactivity of individual nucleophiles. In this regard, protein structure and compartmentalization affect the reactivity of individual nucleophilic centers and will defi ne the molecular targets of electrophilic fatty acids.While enzymatically-oxygenated unsaturated fatty acids typically transduce anti-infl ammatory actions via specifi c g protein-coupled receptor ligand activity ( 2, 3 ), transcriptional responses to electrophilic fatty acids reveal that a broader array of signaling events are instigated ( 4, 5 ). The basis for this pleiotropy resides in the facile Michael addition of electrophilic fatty acid derivatives with nucleophilic centers of proteins that regulate structure and function ( 6 ). Functionally-signifi cant protein targets of electrophilic fatty acids include the transcriptional regulatory protein complex nuclear factor kappa B (NFkB), the

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“…These results are in accordance with several studies that compared an enzymatic method with the non-specific conventional Jaffe method. These results indicate that Jaffe methods, based on an alkaline picrate reaction, overestimate true serum creatinine concentrations due primarily to non-specific protein interference (17)(18)(19).…”

Section: Methods Comparisonmentioning

confidence: 99%

Applicability of common reference intervals for serum creatinine concentrations to the Croatian population

Flegar-Meštrić

Perkov

Šimonović

et al. 2009

Clinical Chemistry and Laboratory Medicine

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Background: In accordance with an ongoing activity for worldwide harmonization based on traceability in laboratory methods, the goal of this study was to validate the applicability of recommended ''common'' reference intervals for serum creatinine concentrations using a specific enzymatic method to the Croatian population. Methods:The reference group consisted of 240 healthy subjects (120 males and 120 females), between 18 and 74 years of age (median 57 years), who were selected in accordance with the International Federation of Clinical Chemistry and Laboratory Medicine (IFCC) recommendations. Creatinine in serum was measured using the creatinine enzymatic assay (Olympus OSR61204) that was standardized to the isotopic dilution mass spectrometry (IDMS) method and National Institute of Standards and Technology (NIST) Standard Reference Material (SRM) 967. In addition, creatinine was measured using a kinetic Jaffe method (Olympus OSR6178) standardized to NIST SRM 909b level 2 standard. Results: Method comparison between enzymatic creatinine (x) and the Jaffe kinetic method (y) gave the following P/B equation for the entire group (ns240): ys1.00xq17.00; rs0.968. Reference intervals for serum creatinine (central 95th percentiles) obtained using the enzymatic creatinine

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Determination of serum creatinine by Jaffe method and how to calibrate to eliminate matrix interference problems

Cited by 53 publications

References 9 publications

Bone marrow mononuclear cells induce beneficial remodeling and reduce diastolic dysfunction in the left ventricle of hypertensive SS/MCWi rats

Bone marrow mononuclear cells induce beneficial remodeling and reduce diastolic dysfunction in the left ventricle of hypertensive SS/MCWi rats

Characterization and quantification of endogenous fatty acid nitroalkene metabolites in human urine

Applicability of common reference intervals for serum creatinine concentrations to the Croatian population

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