Determination of serum creatinine by isotope dilution method using discharge-assisted thermospray liquid chromatography/mass spectrometry

Abstract

A discharge-assisted thermospray (plasmaspray) liquid chromatography/mass spectrometric method for the determination of serum creatinine is described. The method incorporates stable isotope dilution using (D3)creatinine as an internal standard. Separation is performed in reversed-phase high-performance liquid chromatography using 0.01 M aqueous ammonium acetate as a flow solvent. Effluents are directly introduced to the mass spectrometer and [MH]+ ions are monitored during LC/MS using the selected ion monitoring method. Satisfactory agreement between the analytical result and the certified value of the serum sample of standard reference material and relative standard deviation ranging from 0.6% to 1.2% was obtained.

Determination of creatinine in human serum by short‐end injection capillary zone electrophoresis

“…It is one of the most widely used markers of renal function. Although in the clinical laboratory creatinine levels were commonly measured by Jaffé-based spectrophotometric method [2], the search for rapid analytical tools to measuring renal markers has prompted the development of various assays such as HPLC-UV [3][4][5][6][7] and HPLC-MS detection [8][9][10], HPTLC [11,12], CE [13][14][15][16][17], , and enzymatic methods [22,23]. HPLC and CE methods, without precolumn treatments, appear to be the simplest options for analysis of creatinine; in particular the use of CE for the biological fluids analysis in the clinical laboratory is an attractive alternative to many current analytical methods.…”

Isotope dilution-liquid chromatography/electrospray ionization-tandem mass spectrometry for the determination of serum thyroxine as a potential reference method

“…for glucose, 9 cholesterol, 10 creatinine 11 and cortisol 12 in serum and for phencyclidine in urine 13 ) and with APCI-LC/ MS (for cholesterol in serum).…”

GC–MS determination of creatinine in human biological fluids as pentafluorobenzyl derivative in clinical studies and biomonitoring: Inter-laboratory comparison in urine with Jaffé, HPLC and enzymatic assays

“…However, the great interest in creatinine has resulted in many different analytical methods. They include batch and automated spectrophotometric and enzymatic assays as well as instrumental methods including HPLC, GC-MS, LC-MS and LC-MS/MS [9][10][11][12][13][14][15][16][17][18][19][20][21][22][23][24][25].…”