Determination of serum calcium levels by<sup>42</sup>Ca isotope dilution inductively coupled plasma mass spectrometry

Han, Bing; Ge, Menglei; Zhao, Haijian; Yan, Ying; Zeng, Jie; Zhang, Tianjiao; Zhou, Weiyan; Zhang, Jiangtao; Wang, Jing; Zhang, Chuanbao

doi:10.1515/cclm-2017-0175

Cited by 5 publications

(2 citation statements)

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“…Such loss will result in crucial errors in protein quantification. Due to the low ionization efficiency of heteroatoms (such as phosphorus and sulfur which are covalently bonded to the protein's primary structure) and its susceptibility to severe spectral line interference, 24,25 it is difficult to measure the heteroatoms by ICP-MS directly, and the measurement of heteroatoms is also interfered by polyatomic ions. 26,27 In fact, the use of the collision reaction cells and high-resolution ICP-MS instrumentation to remove polyatomic interferences may hinder the applicability of ICP-MS quantification in biological applications, usually requiring low limits of detection (LOD).…”

Section: Absolute Quantification Of Metalloproteins By Using Icp-ms Naturally Present Elements (P S Se and Metals)mentioning

confidence: 99%

Inductively Coupled Plasma Mass Spectrometry For Metrometallomics: The Study Of Quantitative Metalloproteins

Feng¹

2021

At.Spectrosc.

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The emerging field of metrometallomics refers to the qualitative and quantitative measurement of metallic analytes in the metrological sciences. Inductively coupled plasma mass spectrometry (ICP-MS) has the merits of multi-elemental quantification with very low detection limits. It has been applied for the quantification of peptides and proteins by measuring the concentration of certain elements, which is a very important applications area in metrometallomics. This review showcases the application of ICP-MS on the quantification of metalloproteins through the quantification of protein-bound metal/metalloid elements, intrinsic metal/metalloid elements, or labeled metal/metalloid elements. The results of study also suggest that metrometallomics can be a useful tool in the development of certified reference materials of metalloproteins.

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Section: Absolute Quantification Of Metalloproteins By Using Icp-ms Naturally Present Elements (P S Se and Metals)mentioning

confidence: 99%

Inductively Coupled Plasma Mass Spectrometry For Metrometallomics: The Study Of Quantitative Metalloproteins

Feng¹

2021

At.Spectrosc.

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“…The method was established by the National Center of Clinical Laboratory and was described in the previous paper. [ 9 ] All of the samples (48 patient samples and 25 processed materials) were divided into 12 subsets and detected over a period of 12 days.…”

Section: Ethodsmentioning

confidence: 99%

Inductively Coupled Plasma Mass Spectrometry as a Reference Method to Evaluate Serum Calcium Measurement Bias and the Commutability of Processed Materials during Routine Measurements

Zhao

Yin

et al. 2018

Chinese Medical Journal

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Background:Measuring total serum calcium is important for the diagnosis of diseases. Currently, results from commercial kits for calcium measurement are variable. Generally, the performance of serum calcium measurements is monitored by external quality assessment (EQA) or proficiency testing schemes. However, the commutability of the EQA samples and calibrators is often unknown, which limits the effectiveness of EQA schemes. The aim of this study was to evaluate the bias of serum calcium measurements and the commutability of processed materials.Methods:Inductively coupled plasma mass spectrometry was applied as a comparative method, and 14 routine methods were chosen as test methods. Forty-eight serum samples from individual patients and 25 processed materials were quantified. A scatter plot was generated from patient samples, and 95% prediction intervals were calculated to evaluate the commutability of the processed materials and measurement bias at three concentration levels was used to determine the accuracy of routine assays.Results:All assays showed high precision (total coefficient of variation [CV] <2.26%) and correlation coefficients (r > 0.99). For all assays, the mean bias for the 48 patient samples ranged from −0.13 mmol/L to 0.00 mmol/L (−5.61–0.01%), and the ranges for the three concentrations were −0.10–0.04 mmol/L (−5.71–2.35%), −0.14–−0.01 mmol/L (−5.80–−0.30%), and −0.19–0.04 mmol/L (−6.24–1.22%). The EQA samples, calibrators, and animal sera exhibited matrix effects in some assays; human serum pools were commutable in all assays; certificate reference materials were commutable in most assays, and only GBW09152 exhibited a matrix effect in one assay; and aqueous reference materials exhibited matrix effects in most assays.Conclusions:Biases for most assays were within the acceptable range, although the accuracy of some assays needs improvement. Human serum pools prepared from patient samples were commutable, and the other tested materials exhibited a matrix effect.

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Carbon composite thermoplastic electrodes integrated with mini-printed circuit board for wireless detection of calcium ions

Özer

2022

ANAL. SCI.

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Determination of serum calcium levels by⁴²Ca isotope dilution inductively coupled plasma mass spectrometry

Abstract: ID ICP-MS is a simple and accurate candidate reference method for serum calcium measurement and can be used to establish and improve serum calcium reference system in China.

Cited by 5 publications

References 10 publications

Inductively Coupled Plasma Mass Spectrometry For Metrometallomics: The Study Of Quantitative Metalloproteins

Inductively Coupled Plasma Mass Spectrometry For Metrometallomics: The Study Of Quantitative Metalloproteins

Inductively Coupled Plasma Mass Spectrometry as a Reference Method to Evaluate Serum Calcium Measurement Bias and the Commutability of Processed Materials during Routine Measurements

Carbon composite thermoplastic electrodes integrated with mini-printed circuit board for wireless detection of calcium ions

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Determination of serum calcium levels by42Ca isotope dilution inductively coupled plasma mass spectrometry

Abstract: ID ICP-MS is a simple and accurate candidate reference method for serum calcium measurement and can be used to establish and improve serum calcium reference system in China.

Cited by 5 publications

References 10 publications

Inductively Coupled Plasma Mass Spectrometry For Metrometallomics: The Study Of Quantitative Metalloproteins

Inductively Coupled Plasma Mass Spectrometry For Metrometallomics: The Study Of Quantitative Metalloproteins

Inductively Coupled Plasma Mass Spectrometry as a Reference Method to Evaluate Serum Calcium Measurement Bias and the Commutability of Processed Materials during Routine Measurements

Carbon composite thermoplastic electrodes integrated with mini-printed circuit board for wireless detection of calcium ions

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Determination of serum calcium levels by⁴²Ca isotope dilution inductively coupled plasma mass spectrometry