Determination of serotonin and dopamine in mouse brain tissue by high performance liquid chromatography with electrochemical detection

Sasa, S; Blank, Cornelia

doi:10.1021/ac50011a008

Cited by 165 publications

(39 citation statements)

References 23 publications

(55 reference statements)

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“…The use of decerebrate, anesthetized, or analgesicReceived for publication 12 April 1979 and in revised form 17 May 1979. 'Abbreviations used in this paper: AMT, alpha-methyltyrosine; DA, dopamine; 5,7-DHT, 5,7-dihydroxytryptamine; 6-FT, 6-fluorotryptophan; 5HT, 5-hydroxytryptamine; NE, norepinephrine; PCA, para-chloroamphetamine; PCPA, para-chlorophenylalanine. treated animals, although necessary for many of the techniques used to measure ventilation, is open to the criticism that sedation may in itself influence breathing and ventilatory control (3,4).…”

Section: Introductionmentioning

confidence: 99%

Serotonin and the control of ventilation in awake rats.

Olson¹,

Dempsey²,

McCrimmon³

1979

J. Clin. Invest.

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A B S T R A C T In awake, unrestrained, intact rats, reserpine, para-chlorophenylalanine, 6-fluorotryptophan, and para-chloroamphetamine depleted whole brain serotonin and produced a substantial and sustained hyperventilation as evidenced by a 5-9 torr drop in PaC02. Administration of 5-hydroxytryptophan to rats treated with para-chlorophenylalanine partially alleviated the hyperventilation. No change in ventilation was observed after alpha-methyltyrosine. 5,7-Dihydroxytryptamine produced contradictory results. Oni the basis of these pharmacological studies, we propose that some serotonin-mediated nerve transmissions might function under physiological conditions to inhibit the central nervous system output which conitrols normal breathing.

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Section: Introductionmentioning

confidence: 99%

Serotonin and the control of ventilation in awake rats.

Olson¹,

Dempsey²,

McCrimmon³

1979

J. Clin. Invest.

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“…Serum serotonin was determined by liquid chromatography (Sasa and Blank 1977). Urinary 17-OHCS and Furosemide-upright loading.…”

Section: Methodsmentioning

confidence: 99%

Primary aldosteronism in childhood due to primary adrenal hyperplasia.

Kikuta

Sanjo

Nakajima

et al. 1988

Tohoku J. Exp. Med.

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We present an unusual case of primary aldosteronism in childhood.A 9-year-old boy had hypertension, hypokalemia, hyporeninemia and hyperaldosteronism.Dexamethasone administration decreased plasma aldosterone transiently but failed to correct the hyperaldosteronism, excluding dexamethasone-suppressible hyperaldosteronism. Plasma aldosterone decreased with upright posture and showed a circadian rhythm. Spironolactone treatment normalized blood pressure and serum potassium and lowered aldosterone secretion.During the studies, plasma aldosterone correlated with serum cortisol but not with plasma renin. Preoperative results indicated that this patient presented the functional features of aldosteronoma.Adrenal computed tomography, scintigraphy and left venography were not diagnostic of adrenal lesions. The left adrenal venous sampling showed hypersecretion of aldosterone from the left adrenal gland. The left adrenalectomy revealed micronodular hyperplasia but resulted in a prompt and sustained reversal of hypertension and hyperaldosteronism. These findings suggest that primary aldosteronism in this patient resulted from primary adrenal hyperplasia.Thus, adrenal hyperplasia is a heterogenous group of disorders and carefully selected studies allow prospective selection of appropriate treatment. primary aldosteronism ; childhood ; primary adrenal hyperplasia Primary aldosteronism characterized by hypertension, hypokalemia and hyporeninemia is very rare in childhood (Grim et al. 1967 ;Ganguly et al. 1980b ;New and Levine 1980;Bryer-Ash et al. 1984;Oberfield et al. 1984). Typically, three forms of primary aldosteronism are identified : Aldosterone-producing adenoma (APA), idiopathic hyperaldosteronism (IHA) and dexamethasone-

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“…This concentrating technique is similar to the prostaglandin analysis pro cedure used by Dawson et al [3] and allows recovery of essentially 100 % of the radioactivity in the effluent. For analysis of 3H-5HT or 3H-NE, the residue was taken up in 0.01 N HC1 and injected onto a strong cation exchange HPLC system essentially as described by Sasa and Blank [16], When l4C-PGE! was analyzed the residue was taken up in methanolic-acctic acid and injected onto a reverse phase HPLC system as de scribed by Ghias-ud-din et al [5].…”

Section: Methodsmentioning

confidence: 99%

Differences in the Perinatal Development of the Isolated Rabbit Lungs’ Ability to Inactivate Vasoactive Substances

Eb¹,

Rankin²

1983

Neonatology

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A 20-second bolus of serotonin (5-hydroxytryptamine, 5HT), prostaglandin E₁ (PGE₁·) or norepinephrine (NE) was infused into the pulmonary arteries of lungs isolated from 26-day gestation fetal, newborn (within 12 h of birth), and young adult rabbits. In each case the concentration of the infused substrate was much less than the apparent Michaelis constant for the uptake process. At these low concentrations there was no observable vasoactivity. In order to assure a constant ratio of flow to capillary surface area (independent of lung size), perfusate flow was adjusted so that the retention time of a vascular dye was approximately 7 s. Under these conditions the isolated adult rabbit lungs inactivate a similar fraction (40–50%) of each of the substrates. In contrast, isolated newborn rabbit lungs inactivate about 20% of the infused PGE₁, 90% of the 5HT, and 60% of the NE, and the nonventilated, previable fetal rabbit lungs inactivate about 20% of the infused PGE₁ 60% of the 5HT, and 80% of the NE.

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Determination of serotonin and dopamine in mouse brain tissue by high performance liquid chromatography with electrochemical detection

Cited by 165 publications

References 23 publications

Serotonin and the control of ventilation in awake rats.

Serotonin and the control of ventilation in awake rats.

Primary aldosteronism in childhood due to primary adrenal hyperplasia.

Differences in the Perinatal Development of the Isolated Rabbit Lungs’ Ability to Inactivate Vasoactive Substances

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