Determination of self-incompatibility groups of sweet cherry genotypes from Turkey

Abstract

ABSTRACT. Determination of S-allele combinations of sweet cherry genotypes and cultivars has importance for both growers and breeders. We determined S-allele combinations of 40 local Turkish sweet cherry genotypes using a PCR-based method. Ten different S-alleles were detected. Although the most common S-allele was S 3 , as also found in Western genotypes and cultivars, there were some differences in the frequencies of some S-alleles between Turkish and Western sweet cherry genotypes. According to their S-allele compositions, 30 local Turkish sweet cherry genotypes were assigned to 10 previously identified incompatibility groups. For the remaining genotypes, whose S-allele combinations did not fit to any previous incompatibility groups, three more incompatibility groups, XLII, XLIII and XLIV, were proposed. Results obtained from this study will help both sweet cherry growers and breeders to better manage these local Turkish sweet cherry genotypes in their orchards.

Identification of S-genotypes of sweet cherry cultivars from Central and Eastern Europe

“…Amplification of intron II did not make it possible to identify the alleles S 1 , S 3 and S 13 , which is consistent with the results obtained by other authors Schuster et al 2007;Ipek et al 2011). Similarity in the size of the products of amplification of intron I or intron II was also observed for the alleles S 16 and S 32 , S 2 and S 7 , S 9 and S 10 (Szikriszt et al 2013), for the alleles S 2 , S 7 , S 9 and S 12 (Ipek et al 2011;Ercisli et al 2012), and for the alleles S 10 and S 14 (Ipek et al 2011).…”

“…Following the use of the PaConsIF/PaConsIR primers, no product of the amplification of the S 13 allele was obtained, like in the analyses by Sonneveld et al (2003) and Schuster et al (2007). After amplifying intron I, it was impossible to distinguish the alleles S 4 from S 6 , S 1 from S 5 , and S 2 from S 9 , which confirms the results obtained for sweet cherry cultivars from Turkey and Croatia (Ipek et al 2011;Ercisli et al 2012). Amplification of intron II did not make it possible to identify the alleles S 1 , S 3 and S 13 , which is consistent with the results obtained by other authors Schuster et al 2007;Ipek et al 2011).…”

“…Specific primers based on the S-RNase gene were developed for the alleles S 1 -S 16 (Sonneveld et al 2001, and for alleles S 17 -S 19 , S 21 -S 25 , S 34 and S 37 (Szikriszt et al 2013). The effectiveness of the use of consensus primers and specific primers in the identification of the S-alleles of sweet cherry was confirmed in the studies by Békefi et al (2003); De Cuyper et al (2005); Schuster et al (2007); Ipek et al (2011);Ercisli et al (2012); Szikriszt et al (2013).…”

“…At first, 26 incompatibility groups were created (Tobutt et al 2004). As the composition of the S-alleles in other cultivars of sweet cherry was determined, new groups were created (Békefi et al 2003(Békefi et al , 2010Sonneveld et al 2003;Marchese et al 2007;Schuster et al 2007;Gisbert et al 2008;Ipek et al 2011;Schuster 2012;Szikriszt et al 2013). Currently, the cultivars of sweet cherry are assigned to 47 incompatibility groups I-XLVII, to the group of self-compatible cultivars and to the group '0' of unique S-genotypes as universal pollen donors (Schuster 2012).…”

“…Identification of S-alleles was performed for sweet cherry cultivars from Western Europe, for Belgian and British wild cherries, for sweet cherry cultivars and selections gathered in the Swedish gene bank, and for the local Sicilian, Spanish, Greek, Turkish and Croatian cultivars Tobutt et al 2004;De Cuyper et al 2005;Marchese et al 2007;Gisbert et al 2008;Lacis et al 2008;Vaughan et al 2008;Ganopoulos et al 2010;Ipek et al 2011;Ercisli et al 2012;Szikriszt et al 2013;Cachi, Wünsch 2014). S-alleles were also identified in sweet cherry cultivars originating in several countries of Central and Eastern Europe, mostly in the cultivars and selections gathered in the Latvian collection, and also in the Hungarian cultivars, but in only a few of the Czech and Ukrainian cultivars (Békefi et al 2003(Békefi et al , 2010Schuster et al 2007;Lacis et al 2008;Schuster 2012).…”