Determination of selected components in seminal plasma of donkey stallions and their correlation to semen quality parameters

Vyvial, Michal; Horácková, E; Sedlinská, Markéta; Jánová, Eva; Krisová, Šárka; Mráčková, Miroslava

doi:10.2754/avb201988040377

Cited by 5 publications

(9 citation statements)

References 22 publications

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“…Interestingly, to date, no studies have reported zinc and magnesium levels in the seminal plasma of Poitou jackasses. Thus, the present study reports the seminal concentrations of zinc and magnesium in this breed, where the zinc level was similar and the magnesium level was higher than those found in other domestic donkeys by Vyvial et al (37).…”

Section: Discussionsupporting

confidence: 82%

“…Also, low concentrations of glucose (0.0-0.2 mmol/L), cholesterol (0.1-0.3 mmol/L), zinc (18.1-23.7 µmol/L), and magnesium (1.6-2.8 mmol/L) were found. Zinc is a micromineral that plays an important role in many protective sperm properties, such as membrane stabilization, and antioxidant and antibacterial functions (36), and a testicular and epididymal origin has been suggested in Equidae (11,37). Magnesium is a cation related to high seminal plasma quality in boars because it improves viability by reducing sperm membrane damage (38).…”

Section: Discussionmentioning

confidence: 99%

“…The AST and ALP have been related to membrane sperm integrity in humans (42). In donkeys, AST and ALP are secreted from testis and epididymis, while GGT's origin is not clear because it does not correlate with volume or sperm concentration (37). In stallion, GGT has a testicular and epididymal origin, but since it is species-specific, this information may not apply to jackass (11).…”

Section: Discussionmentioning

confidence: 99%

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Semen Quality and Freezability Analyses in the Ejaculates of Two Poitou Donkeys in the Southern Hemisphere

et al. 2021

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The Baudet du Poitou is a vanishing donkey breed recognized for engendering robust working mules. In Chile, only two pure breed Poitou males exist, which belong to the Chilean army and are used for mule production. We performed an extensive sperm and seminal analysis of these two jackasses aged 3 and 6 years and investigated the use of a simple hypometabolic extender for sperm cryopreservation. Computer-assisted sperm analysis showed high motility, velocity, and linearity in sperm movement. The seminal plasma analysis revealed that sodium and chloride were the main electrolytes, and globulins were the main metabolites. Active and variable enzymatic activity was observed. New information is reported about gamma-glutamyltransferase, aspartate aminotransferase, zinc, and magnesium concentrations in seminal plasma of Poitou donkeys. Ejaculates among jackasses showed some variability due to individual variability and different stages in sexual maturation according to age. The freezability index analysis based in viability, total motility and progressive motility with Botucrio extender (57.1 ± 11.0%; 56.6 ± 20.0%; and 22.6 ± 10.3%, respectively) were significantly higher (p < 0.05, p < 0.0001, and p < 0.0001, respectively) than with HM-0 extender (42,6 ± 11.4%; 14.9 ± 5.1%; and 1.0 ± 2.5%, respectively). We report new information on Poitou donkey semen and cryopreservation in the Southern Hemisphere that could be useful in donkey breeding and conservation programs to develop strategies that improve the effectiveness of population management of this breed.

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Section: Discussionsupporting

confidence: 82%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Semen Quality and Freezability Analyses in the Ejaculates of Two Poitou Donkeys in the Southern Hemisphere

et al. 2021

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“…Progressive motility of spermatozoa (alongside semen concentration) is a good indicator of male fertility and both general state of health and health of the reproductive tract (Vyvial et al 2019). Reduced sperm motility parameters are frequently associated with inflammation and severe infection (Björndahl 2010;Vitula et al 2011).…”

Section: Discussionmentioning

confidence: 99%

Fresh semen characteristics in captive accipitrid and falconid birds of prey

Piaček¹,

Zukal²,

Seidlová³

et al. 2020

Acta Vet. Brno

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Artificial insemination (AI) is the most frequently used assisted reproductive technique for captive propagation of rare avian species. As semen quality is critical for reproductive success, baseline data are needed for evaluating and selecting the best male bird donors. To this end, we used computer-assisted semen analysis to assess male eastern imperial eagles (n = 7), northern goshawks (n = 24) and peregrine falcons (n = 20). While imperial eagles and northern goshawks donate ejaculate voluntarily, peregrine falcons required cloacal massage. Eight peregrine falcon females were inseminated with semen from eight males, with fresh ejaculates (15 to 50 µl) applied to the pars uterina of the oviduct immediately after collection and examination. All females were inseminated within 2 h of laying an egg. A fertilization rate of 70% was achieved using this method. Minimum semen characteristics associated with egg fertilization included a semen concentration of 115.12 × 106/ml, 33.52% total motility, 1.92% spermatozoa with progressive motility and 0.17% with rapid motility. Comparative data on spermatozoa concentration and kinematics suggest that eastern imperial eagles concentrate on high quality semen investment at the start of the breeding season, northern goshawks compensate for a decrease in motility-associated parameters with increased semen concentration and peregrine falcons maintain semen production standards throughout the breeding season. Our data show that, in birds of prey, levels of egg fertilization following AI with fresh semen can be almost as successful as after natural mating.

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“…In preserved stallion semen, the exposure to BSP proteins from seminal plasma causes continuous cholesterol removal from the sperm membrane, increasing sperm sensitivity, which can render sperm extremely sensitive to cryopreservation (Calvete et al, 1994;Bergeron et al, 2004). In donkey semen, despite some reports described seminal plasma general composition (Talluri et al, 2017;Vyvial et al, 2019), there is not much available information on seminal plasma proteins. Some studies showed that seminal plasma dilution or removal from donkey semen increases sperm motility and membrane integrity after storage (Serres et al, 2002;Miró et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Effect of egg yolk-based extender and seminal plasma removal on sperm viability of cooled donkey semen

Alonso

Castañeira

Bragulat

et al. 2021

Braz. J. Vet. Res. Anim. Sci.

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Developing effective cooled semen protocols is essential to increase pregnancy rates and reproductive efficiency in donkeys. This study aimed to evaluate the effect on sperm kinetic parameters and membrane integrity in cooled donkey semen diluted with defined milk proteins extender with 1% or 2% of egg yolk and the removal of seminal plasma. Twenty-four ejaculates from six jackasses were collected. Each ejaculate was divided into four aliquots that were diluted in extender with 1% (EY1) or 2% (EY2) egg yolk. One sample from each group was centrifuged, seminal plasma was removed (CEY1, CEY2 groups, respectively), and the samples were then refrigerated at 5 °C for 24 h. Fresh and cooled semen samples were assessed for sperm motility, morphology, and plasma membrane integrity. Total motility, progressive motility, sperm kinetic parameters, or live sperm cells were not statistically different when semen was cooled with an extender supplemented with 1% or 2% of egg yolk. Seminal plasma removal does not affect total motility or sperm kinetic parameters. However, progressive motility decreased (P<0.05) when semen was extended with 2% of egg yolk and seminal plasma was removed. Membrane integrity was affected (P<0.05) in centrifuged samples. In conclusion, the obtained results suggest that there is no difference in sperm kinetics and membrane integrity when 1% or 2% of egg yolk was added to the Equiplus(R) extender. Also, the removal of seminal plasma by centrifugation did not have any beneficial effect on cooled donkey semen. Further studies are needed to relate these results with in vivo fertility tests with cooled donkey semen.

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Determination of selected components in seminal plasma of donkey stallions and their correlation to semen quality parameters

Cited by 5 publications

References 22 publications

Semen Quality and Freezability Analyses in the Ejaculates of Two Poitou Donkeys in the Southern Hemisphere

Semen Quality and Freezability Analyses in the Ejaculates of Two Poitou Donkeys in the Southern Hemisphere

Fresh semen characteristics in captive accipitrid and falconid birds of prey

Effect of egg yolk-based extender and seminal plasma removal on sperm viability of cooled donkey semen

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