Determination of Seasonal Variation of Volatile Organic Constituents of the Leaves of Traditional Herb Ocimum sanctum Linn

Abstract

In the traditional system of medicine Ocimum sanctum Linn. is used to cure diseases like dysentery, bronchitis, malaria, bronchial asthma, diarrhea, arthritis, skin diseases, painful eye diseases, chronic fever. The essential oil obtained by using hydrodistillation of the leaves of Ocimum sanctum Linn. (purple variety) is collected throughout 1 y and analyzed employing gas chromatography equipped with flame ionization detector and gas chromatography connected with mass spectrometry. The yield of essential oil varied in the range from 0.14 to 0.21 % (w/w). 23 to 39 constituents were identified which represented 95.39±0.38 % of the total oil. The most abundant compound was identified as methyl eugenol with an average of 69.88±0.09 % with varying quantities from 55.48±0.36 to 76.78±0.54 %. The other constituents were beta-caryophyllene 10.40±0.06 %, alpha-ylangene 4.18±0.04 %, gamma-muurolene 3.64±0.04 % and borneol 2.02±0.01 %. The oil was found to be rich in phenylpropanoid (71.98±0.27 %) type constituents (average of 12 mo). A high amount of methyl eugenol (76.78±0.54 %) was observed during November while low (55.48±0.36 %) in April. It was noticed that when the plant was matured in April the accumulation of sesquiterpenes increased and phenylpropanoids decreased.

Anti-Dengue Activity of Lipophilic Fraction of Ocimum basilicum L. Stem

“…The injector temperature and injection volume were 290 °C and 1.0 μL, respectively; split ratio 1:50. The mass scan range for the MS spectrum was 50–650 amu at 70 eV [ 36 , 37 , 38 , 39 , 40 ]. Identification of constituents was made using the supported retention index (RI) regarding the homologous series of n -alkanes C 8 -C 25 including identical experimental conditions on the ZB-5 column, a NIST and WILEY MS library search, and via examination with the MS literature information [ 41 ] and co-injection of economic samples with ≥98% purity.…”

Evaluation of Morphological Characteristics, Yield Potential, and Essential Oil Composition of Ocimum sanctum Genotypes for Crop Improvement

“…Other significant metabolites such as caryophyllene, linalool, and ursolic acid further contribute to tulsi's adapt genic and stress-relieving properties. Collectively, these metabolites interact to provide a range of health benefits, encompassing immune system modulation, cardiovascular support, and cognitive enhancement [8].…”