Determination of sarcosine based on magnetic cross-linked enzyme aggregates for diagnosis of prostate cancer

Hu, Qianqian; Chen, Guoning; Han, Jili; Wang, Lu; Cui, Xia; Wang, Peilong; Chang, Chun; Fu, Qiang

doi:10.1016/j.bej.2021.108039

Cited by 7 publications

(8 citation statements)

References 43 publications

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“…Nevertheless, 95.6% of CLEAs degradation occurred overall, while mCLEAs degradation was 86.4%. The difference in thermal degradation of 9.2% was attributed to the presence of MNPs in mCLEAs, which generally show excellent thermal stability ( Hu et al, 2021 ). The physical–chemical characterization of γ-Fe 2 O 3 MNPs, which has been used in recent research, was performed using various analyses in our previous work ( Šulek et al, 2010 ).…”

Section: Resultsmentioning

confidence: 99%

“…A high content of free amino groups, especially on the surface of an enzyme, is crucial to create strong intermolecular bonds between the free amino and polymers or oligomers resulting from the aldol condensation of glutaraldehyde. Moreover, the addition of BSA, which forms co-aggregates with an enzyme containing few lysine residues, can form CLEAs with high activity and improved mechanical properties ( Hakimi et al, 2019 ) Also, the resistance to extreme pHs presented by CLEAs was a result of the newly formed secondary interactions between glutaraldehyde and some catalytic relevant amino acids of the enzyme ( Hu et al, 2021 ). Although the synthesis of CLEAs is an efficient method for enzyme immobilization, there are a few limitations, such as low mechanical stability if the crosslinking was not appropriate, resulting in the leaching of the enzyme.…”

Section: Introductionmentioning

confidence: 99%

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The Synthesis of (Magnetic) Crosslinked Enzyme Aggregates With Laccase, Cellulase, β-Galactosidase and Transglutaminase

Podrepšek

Knez

Leitgeb

2022

Front. Bioeng. Biotechnol.

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Immobilized enzymes have important aspects due to the fact that they possess higher stability, have the possibility to be easily removed from the reaction mixture, and are much easier to use when compared to free enzymes. In this research, the enzymes laccase, cellulase, β-galactosidase (β-gal), and transglutaminase (TGM) were immobilized by two different methods: crosslinked enzyme aggregates (CLEAs) and magnetic crosslinked enzyme aggregates (mCLEAs). The processes for CLEAs and mCLEAs preparation with different enzymes have been optimized, where the aim was to achieve the highest possible relative activity of the immobilized enzyme. The optimal conditions of the synthesis of CLEAs in mCLEAs are described, thus emphasizing the difference between the two types of immobilization based on different enzymes. This comparative study, which represents the synthesis of crosslinked enzyme aggregates using different enzymes, has not been performed so far. Moreover, the obtained activity of CLEAs and mCLEAs is presented, which is important for further use in different biocatalytic processes. Specifically, of a higher importance is the selection of enzymes involved in immobilization, as they belong to the three different most applicable enzymes (oxidoreductases, hydrolases, and transferases). The study confirmed that the resulting activity of the immobilized enzyme and the optimization of enzyme immobilization depended on the type of the enzyme. Moreover, the prepared CLEAs and mCLEAs were exposed to the supercritical carbon dioxide (scCO2) at different pressures to determine the effect of scCO2 on enzyme activity in immobilized form. Additionally, to demonstrate the reuse and stability of the immobilized enzyme, the stability and reusability tests of CLEAs and mCLEAs were performed. The catalytic performance of immobilized enzyme was tested, where the catalytic efficiency and long-term operational stability of mCLEAs were obviously superior to those of CLEAs. However, the higher activity observed for CLEAs compared to mCLEAs suggests a significant effect of magnetic nanoparticles in the stabilization of an enzyme crosslinked aggregate structure.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The Synthesis of (Magnetic) Crosslinked Enzyme Aggregates With Laccase, Cellulase, β-Galactosidase and Transglutaminase

Podrepšek

Knez

Leitgeb

2022

Front. Bioeng. Biotechnol.

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“…Our method enables the rapid detection of sarcosine with a reaction time of ∼10 min, which is less than the assay time in our previous work. 20 Since the experimental equipment and reagents used in this work are inexpensive, it is affordable for many laboratories and community-level medical institutions, which facilitates the promotion of this method and the screening of PCa. Furthermore, because of the remarkable specificity and excellent anti-interference properties, the pre-treatment process for this method is very simple and easy to perform.…”

Section: Resultsmentioning

confidence: 99%

“…Hydrogen peroxide is one of the reaction products of sarcosine and sarcosine oxidase (SOX), and it can be detected using fluorescence or colorimetric methods to reflect the concentration of sarcosine involved in this reaction. In our previous work, 20 the determination of sarcosine was accomplished using a colorimetric method, providing an approach for the analysis of sarcosine without the need for complicated pretreatment. In order to achieve the more rapid and accurate detection of sarcosine, SOX was embedded into ZIF-8 to form SOX@ZIF-8 in this study, which has a better stability than the free enzyme.…”

Section: Introductionmentioning

confidence: 99%

Nanoreactor of sarcosine oxidase-embedded ZIFs activates fluorescent response for diagnosis of prostate cancer

Chen

Wang

et al. 2022

New J. Chem.

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“…The enzyme's kinetic parameter, in the form of a Michaelis-Menten constant, was obtained according to the Michaelis-Menten equation [28]:…”

Section: Evaluation Of the Embedded Glucose Oxidasementioning

confidence: 99%

Enzyme Encapsulation by Facile Self-Assembly Silica-Modified Magnetic Nanoparticles for Glucose Monitoring in Urine

et al. 2022

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Silica nanoparticles hold tremendous potential for the encapsulation of enzymes. However, aqueous alcohol solutions and catalysts are prerequisites for the production of silica nanoparticles, which are too harsh for maintaining the enzyme activity. Herein, a procedure without any organic solvents and catalysts (acidic or alkaline) is developed for the synthesis of silica-encapsulated glucose-oxidase-coated magnetic nanoparticles by a facile self-assembly route, avoiding damage of the enzyme structure in the reaction system. The encapsulated enzyme was characterized by scanning electron microscopy, transmission electron microscopy, energy-dispersive spectrometry, and a vibrating sample magnetometer. Finally, a colorimetric sensing method was developed for the detection of glucose in urine samples based on the encapsulated glucose oxidase and a hydrogen peroxide test strip. The method exhibited a good linear performance in the concentration range of 20~160 μg mL−1 and good recoveries ranging from 94.3 to 118.0%. This work proves that the self-assembly method could be employed to encapsulate glucose oxidase into silica-coated magnetic particles. The developed colorimetric sensing method shows high sensitivity, which will provide a promising tool for the detection of glucose and the monitoring of diabetes.

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Determination of sarcosine based on magnetic cross-linked enzyme aggregates for diagnosis of prostate cancer

Cited by 7 publications

References 43 publications

The Synthesis of (Magnetic) Crosslinked Enzyme Aggregates With Laccase, Cellulase, β-Galactosidase and Transglutaminase

The Synthesis of (Magnetic) Crosslinked Enzyme Aggregates With Laccase, Cellulase, β-Galactosidase and Transglutaminase

Nanoreactor of sarcosine oxidase-embedded ZIFs activates fluorescent response for diagnosis of prostate cancer

Enzyme Encapsulation by Facile Self-Assembly Silica-Modified Magnetic Nanoparticles for Glucose Monitoring in Urine

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