Determination of rifampicin and its main metabolite in plasma and urine in presence of pyrazinamide and isoniazid by HPLC method

Panchagnula, Ramesh; Sood, Anurag; Sharda, Nishi; Kaur, Kanwal J.; Kaul, C. L.

doi:10.1016/s0731-7085(98)00112-5

Cited by 65 publications

(35 citation statements)

References 15 publications

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“…To date, there are a number of published analytical methods [2][3][4][5][6][7][8][9][10], some that have been used to determine plasma rifampicin concentrations, but none used to determine cell associated rifampicin. Further, previous publications often have not been used on plasma samples [3,8,10], have not used and internal control [2][3][4][5], or have limited sensitivity [8,9]. Here a validated, accurate, precise and sensitive HPLC-MS method has been developed for the detection of rifampicin in both plasma and cells.…”

Section: Discussionmentioning

confidence: 99%

“…A number of HPLC-based assays for rifampicin have been described [2][3][4][5][6][7][8][9][10], but these have lacked standardisation. For some assays an internal standard was not included [2][3][4][5], or the assays were not suitable for analysis of plasma (lacking an work-up procedure) [6,7] or else required large volumes of sample with relatively low sensitivity [8,9]. Only one previous HPLC-MS based assay has been reported [10], and lack of internal standard and validated workup procedure does not make this suitable for analysing drug concentrations in plasma or cells.…”

Section: Introductionmentioning

confidence: 99%

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A rapid and sensitive HPLC–MS method for the detection of plasma and cellular rifampicin

Hartkoorn

Khoo

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et al. 2007

Journal of Chromatography B

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A rapid and sensitive HPLC–MS method for the detection of plasma and cellular rifampicin

Hartkoorn

Khoo

Back

et al. 2007

Journal of Chromatography B

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“…The sample was then centrifuged for 30 min at 4,000 rpm and 1 ml aliquot taken from supernatant and analyzed using validated reversed phase HPLC method (Model SCL-10APV, Shimadzu, Japan) modified from Panchagnula et al (1999) (33).…”

Section: Determination Of Drug Content and Loading Efficiencymentioning

confidence: 99%

Formation of Inhalable Rifampicin–Poly(l-lactide) Microparticles by Supercritical Anti-solvent Process

2008

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Abstract. Formation of inhalable microparticles containing rifampicin and poly(L-lactide) (L-PLA) by using supercritical anti-solvent process (SAS) was investigated. The solutions of drug and polymer in methylene chloride were sprayed into supercritical carbon dioxide. The effect of polymer content and operating conditions, temperature, pressure, carbon dioxide molar fraction, and concentration of solution, on product characteristics were studied. The prepared microparticles were characterized with respect to their morphology, particle size and size distribution, drug content, drug loading efficiency, and drug release characteristic. Discrete, spherical microparticles were obtained at high polymer:drug ratios of 7:3, 8:2, and 9:1. The shape of L-PLA microparticles became more irregular and agglomerated with decreasing polymer content. Microparticles with polymer content higher than 60% exhibited volumetric mean diameter less than 5 μm, but percent drug loading efficiency was relatively low. Drug-loaded microparticles containing 70% and 80% L-PLA showed a sustainable drug release property without initial burst release. Operating temperature level influenced on mean size and size distribution of microparticles. The operating pressure and carbon dioxide molar fraction in the range investigated were unlikely to have an effect on microparticle formation. An increasing concentration of feed solution provided larger size microparticles. Rifampicin-loaded L-PLA microparticles could be produced by SAS in a size range suitable for dry powder inhaler formulation.

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“…However, few pharmacokinetic studies and clinical reports so far about the concentrations of antituberculosis drugs in vertebral foci have been published [12,15]. Therefore, high-pressure liquid chromatography (HPLC) method was adopted on the basis of literatures [10,17] to determine the concentrations of isoniazid (INH), rifampicin (RFP) and pyrazinamide (PZA) in pathological vertebrae, serum as well as the normal osseous tissue, in order to provide the theoretical basis for the improvement in treating spinal tuberculosis.…”

Section: Introductionmentioning

confidence: 99%

Measurement of the concentration of three antituberculosis drugs in the focus of spinal tuberculosis

Wang

Wei

2008

Eur Spine J

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This is an experimental study on the distribution of antituberculosis drugs such as rifampin, isoniazid, and pyrazinamide in pathologic vertebrae of spinal tuberculosis in order to provide the regimen of chemotherapy and surgical treatment of spinal tuberculosis. The distribution of antituberculosis drugs in pathologic vertebral tissues matters greatly to the clinical effect of spinal tuberculosis' treatment. However, few pharmacokinetic studies and clinical reports about the concentrations of antituberculosis drugs in vertebral foci have been published so far. Twenty-four patients with spinal tuberculosis were divided into sclerotic group (n = 15) or non-sclerotic group (n = 9) according to radiographic features of lesion. All patients received chemotherapy with 2HRZ/2Á5H 2 R 2 Z 2 for a duration of 4.5 months. Four weeks after chemotherapy all patients underwent surgery and the specimen of serum, ilium, and pathologic vertebral tissues, including sclerotic wall, subnormal osseous tissue, and foci were obtained during operation in 120-130 and 180-190 min after oral intake in the morning, respectively. The levels of three drugs in the specimen were measured using HPLC method. The concentration levels of isoniazid, rifampin and pyrazinamide varied greatly in different tissues of spinal tuberculosis, of which the bactericidal concentration values of isoniazid and rifampin and fivefold minimal inhibitory concentration (MICs) of pyrazinamide were found in subnormal vertebral bone and self-contrast ilium, the MICs of all drugs were found in sclerotic wall outside foci, and undetected level was found in foci inside the sclerotic wall. To patients without vertebral sclerotic wall around the foci, the isoniazid in foci was of bactericidal level and rifampin and pyrazinamide in foci corresponded to the MICs respectively. The sclerotic bone of affected vertebra plays an important role in blocking the antituberculosis drug's penetration into tuberculosis focus.

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Determination of rifampicin and its main metabolite in plasma and urine in presence of pyrazinamide and isoniazid by HPLC method

Cited by 65 publications

References 15 publications

A rapid and sensitive HPLC–MS method for the detection of plasma and cellular rifampicin

A rapid and sensitive HPLC–MS method for the detection of plasma and cellular rifampicin

Formation of Inhalable Rifampicin–Poly(l-lactide) Microparticles by Supercritical Anti-solvent Process

Measurement of the concentration of three antituberculosis drugs in the focus of spinal tuberculosis

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