Determination of Riboflavin in Human Plasma by Excitation-Emission Matrix Fluorescence and Multi-Way Analysis

Abstract

Direct determination of riboflavin (Fig. 1), a vitamin, in human plasma was accomplished based on excitation-emission matrix (EEM) fluorescence measurements and multi-way chemometrics method based on parallel factor analysis (PARAFAC). The PARAFAC trilinear model, without restrictions and using one factor was used in the data analysis. The excitation wavelength range was from 380 to 460 nm and the emission was recorded from 480 to 600 nm. The calibration set was constructed with sixteen standard solutions in a concentration range of 0.02-0.38 mg mL -1 for riboflavin. The capabilities of the method for the analysis were evaluated by determination of riboflavin in synthetic and real samples with satisfactory results. The accuracy of the methods, evaluated through the root mean square error of prediction (RMSEP), was 0.0059 for riboflavin by the PARAFAC model. Also, partial least squares (PLS) model was built at one excitation wavelength and used to determine a set of synthetic and real samples. The best model was obtained with PARAFAC. This result shows that molecular fluorescence spectroscopy can be used for the development of robust analytical methods for the direct determination of riboflavin in complex backgrounds such as human plasma.

Calibration, standardization, and quantitative analysis of multidimensional fluorescence (MDF) measurements on complex mixtures (IUPAC Technical Report)

“…These include, amongst others, the characterization of natural organic matter in soil and aquatic ecosystems [72,108,109], oil-spill forensics [110] and oil characterization [6,111], monitoring of food quality and production [67,71,112], and diagnostics on body fluids e.g. [113,114]. In the near future, we anticipate that there will be a significant increase in the use of EEM/SFS and chemometrics for routine and high-value analytical applications.…”

A perspective on the growth of chemometrics in Iran: a glance into activities between 2005 and 2012

“…Niazi and Ghasemi [153,154] applied PARAFAC for the determination of captopril in pharmaceutical preparations and biological fluids by ultraviolet spectrophotometry. Spectrophotometric determination of tetracycline in pharmaceutical formulation and biological fluids [155] and determination of riboflavin in human plasma by excitationemission matrix fluorescence and multiway analysis [156] were reported by Niazi et al It should be noted that the cited articles were selected from among nearly 44 papers the multiway field that have been published by Iranian chemometricians.…”

Genetic Algorithms-Based Approach for Wavelength Selection in Simultaneous Spectrofluorimetric Determination of Ofloxacin and Riboflavin in biological samples by Partial Least-Squares

“…For ofloxacin, some reported methods include high performance liquid chromatography (HPLC) [1][2][3], TLC [4], differential pulse polarography [5], fluorimetry [6][7][8], spectrophotometry [9,10], capillary electrophoresis [11,12], and flow injection analysis [13,14]. For riboflavin, some analytical procedures that have been used are fluorimetry [15,16], spectrophotometry [17,18], HPLC [19,20], and capillary electrophoresis [21].…”