Determination of residual trifluoroacetate in protein purification buffers and peptide preparations by ion chromatography

Kaiser, Edward; Rohrer, Jeff

doi:10.1016/j.chroma.2004.03.044

Cited by 21 publications

(12 citation statements)

References 4 publications

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“…In addition to these two physiologically tolerable anions, hazardous trifluoroacetic acid (TFA) ones used in the synthesis and/or purification procedures may occur in the preparations of peptides. Furthermore, acetic acid (AcOH) and TFA can be found in synthetic peptide samples as impurities [1][2][3][4][5][6][7][8]. The efficiency of a synthetic peptide is diminished in the presence of a large amount of inactive species, including the counter-ions and impurities.…”

Section: Introductionmentioning

confidence: 99%

Determination of counter‐ions in synthetic peptides by ion chromatography, capillary isotachophoresis and capillary electrophoresis

Mrozik

Markowska

Guzik

et al. 2012

Journal of Peptide Science

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The utility of three various analytical techniques [ion chromatography (IC), capillary electrophoresis (CE) and isotachophoresis (ITP)] was tested in the determination of counter-ions in synthetic peptides. The analyzed ions were acetates, trifluoroacetates and chlorides. IC provided the best results; CE, except limit of detection and limit of quantification, exhibited the comparable results. ITP was classified as the less useful because of the problem with the determination of the chloride ions. Nevertheless, all the three techniques were able to analyze trifluoroacetates and acetates ions with satisfactory results. Except analytical methods, three procedures using hydrochloric acid (HCl) (at two different concentrations) and acetic acid as sample solvents processed by lyophilization were tested. It has been found that the lyophilization not only by HCl but also by acetic acid is a simple and cheap procedure for removal of toxic trifluoroacetic counter-ions from peptides on satisfactory levels.

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Section: Introductionmentioning

confidence: 99%

Determination of counter‐ions in synthetic peptides by ion chromatography, capillary isotachophoresis and capillary electrophoresis

Mrozik

Markowska

Guzik

et al. 2012

Journal of Peptide Science

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“…3,4 Furthermore, TFA is implemented in the peptide purification step via high-performance liquid chromatography (HPLC) as an ion pairing reagent to improve the separation of polar compounds on reversed-phase (RP) columns. [5][6][7][8] As shown previously, TFA shows a tremendous impact on the molecular weight and solubility of peptides. Additionally, TFA can influence secondary structure elements of peptides and shows cell-toxicity, which results in interference in bioassays.…”

Section: Introductionmentioning

confidence: 59%

Simultaneous Quantification of Commonly Used Counter Ions in Peptides and Active Pharmaceutical Ingredients by Mixed Mode Chromatography and Evaporative Light Scattering Detection

Streuli

Coxon

2021

Journal of Pharmaceutical Sciences

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“…Several examples of application of IC in the analysis of pharmaceuticals can be found in the recent scientific literature [1][2][3][4][5][6]. A large number of IC col-*Address correspondence to this author at the Omega Laboratory, 11177 Hamon, Montréal, Québec, H3M 3E4, Canada; Tel: 1-514-335-0310, ext.…”

Section: Ion Chromatography With Suppressed Conductivity Detectionmentioning

confidence: 98%

Analytical Methods for Determination of Counter-ions in Pharmaceutical Salts

Rocheleau¹

2008

CPA

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Inorganic ions as well as organic acids or bases are frequently employed as counter-ions of ionizable drug substances. Therefore, analytical methods are required to confirm production of the correct salt form and to quantify the level of the counter-ion present in each batch of drug substances manufactured. Several options exist for determination of counter-ions in pharmaceutical salts and will be discussed in this review. Ion chromatography (IC) with conductivity detection is probably the most frequently employed method for ion determination. Other techniques such as capillary electrophoresis (CE) represent an alternative to IC for determination of counter-ions in pharmaceutical salts. Due to the lack of chromophores, detection of small ions in CE is typically performed using indirect UV detection. Hydrophilic interaction chromatography (HILIC) with evaporative light-scattering (ELSD) or refractive index detection can also be used for determination of non-UV absorbing ions. Each technique will be described and their advantages highlighted. Current and future trends in measuring counter-ions in pharmaceutical salts will also be discussed.

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Determination of residual trifluoroacetate in protein purification buffers and peptide preparations by ion chromatography

Cited by 21 publications

References 4 publications

Determination of counter‐ions in synthetic peptides by ion chromatography, capillary isotachophoresis and capillary electrophoresis

Determination of counter‐ions in synthetic peptides by ion chromatography, capillary isotachophoresis and capillary electrophoresis

Simultaneous Quantification of Commonly Used Counter Ions in Peptides and Active Pharmaceutical Ingredients by Mixed Mode Chromatography and Evaporative Light Scattering Detection

Analytical Methods for Determination of Counter-ions in Pharmaceutical Salts

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