Determination of Residual Clopidol in Chicken Muscle by Capillary gas Chromatography-negative Chemical Ionization-Mass Spectrometry

Abstract

A selective, sensitive gas chromatography-mass spectrometry (GC-MS) method with negative chemical ionization (NCI) was developed for the detection and quantification of clopidol in chicken muscle. Chicken muscle samples were extracted with acetonitrile and concentrated to dryness; the residue was redissolved in ethyl acetate and applied to an Alumina B cartridge for cleanup. The residue was derivatized with Sylon BFT TM and analyzed by GC-NCI-MS. The selected-ion monitoring mode was performed at m/z values of 156, 158, 191, and 193. The differences in the ratios for the standards and spikes in chicken muscle were within the acceptability criteria. All recoveries of the drug from chicken muscle spiked at 0.5, 5.0 and 50.0 μg/kg were 74.5 -95.6% intra-day, and 71.6 -94.8% inter-day, respectively, with relative standard deviations being lower than 15%. The limits of detection and quantitation were 0.1 and 0.5 μg/kg, respectively. The NCI mode had better selectivity and sensitivity than the electron impact (EI) mode for clopidol.

Determination of coccidiostat clopidol on an electropolymerized-molecularly imprinted polypyrrole polymer modified screen printed carbon electrode

“…Clopidol residue can be commonly determined by liquid chromatography (LC) with UV detection; 3 gas chromatography (GC) with electron capture detection; 4 or LC with mass spectrometry (MS) detection. Analytical methods for clopidol residue detection in chicken tissues consist of diazomethane derivatization followed by gas chromatography (GC), [5][6][7] propionic anhydride derivatization followed by GC, acetic anhydride derivatization followed by GC and highperformance liquid chromatography (HPLC). 8 The objective of high specicity together with high sensitivity can be realized by the concept of MIPs.…”

Development of an enzyme-linked immunosorbent assay for detection of clopidol residues in chicken tissues

“…This has resulted in the development of a broad range of methods to detect CLOP residues in biological matrices. Conventional analytical methods include high‐performance liquid chromatography (HPLC), liquid chromatography/tandem mass spectrometry (LC/MS/MS) and gas chromatography/tandem mass spectrometry (GC/MS/MS) . These methods include multistep procedures such as hydrolysis, extraction, derivative formation and purification.…”

Simultaneous Determination of Azoles Antifungal Drugs in Chicken Tissues by Ultra-performance Liquid Chromatography Tandem Mass Spectrometry

“…However, currently reported analytical methods were mainly focused on anti-bacterial and antiparasitic drugs, while relatively little attention was paid to the antifungal aspects. [1][2][3][4][5][6][7] Antifungal drugs are medications used to treat or prevent fungal infections. Azoles are one type of the most commonly used veterinary antifungal drugs.…”