Determination of Residual Avoparcin in Chicken Muscle by High Performance Liquid Chromatography

Kadota, Minoru; Imanaka, Masaaki; Ogawa, Naoya; Kumashiro, Kazuo; Mori, Tadashige; Oka, Hisao; Ikai, Yoshitomo; Horie, Masakazu; Suzuki, Sukeji; Nakazawa, Hiroyuki

doi:10.3358/shokueishi.35.23

Cited by 5 publications

(5 citation statements)

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“…Moreover, a triethylammonium phosphate mobile phase was used in this HILIC method of AV detection, which is not suitable for ESI-MS/ MS ionization. On the other hand, Kadota et al [5,6] of AV (Fig. 3).…”

Section: Lc-ms/ms Detection Of Av-a and -Bmentioning

confidence: 99%

“…A water-based extraction solvent for AV is better than an organic solvent because of the water-solubility of AV [7]. In this study, we investigated several extraction solvents (the levels of spiked AV: 0.1 lg/g chicken muscle sample), such as water, 0.2 M H 2 SO 4 in water/methanol (40:60 v/v) [5,6], water/ethanol (70:30 v/v) [7], 1 and 5% TFA in water, 5% acetic acid, and 5% formic acid. Table 3 shows that 5% TFA resulted in higher AV recovery from chicken tissues than other solvent extractions.…”

Section: Investigation Of Sample Preparation Of Avmentioning

confidence: 99%

“…These 25 mL of water, 0.2 M H 2 SO 4 in water/ methanol (4:6 v/v) [5,6], water/ethanol (7:3 v/v) [7], 1, 3, and 5% TFA in water, 5% formic acid or 5% acetic acid was added to the centrifuge tube, the mixture was homogenized for 1 min using a YELLOW LINE, D125 basic (IKAm Japan K.K., Nara, Japan) and then the mixture was centrifuged at 6000 rpm (68426g) for 20 min without 0.2 M H 2 SO 4 in water/methanol (4:6 v/v). When 0.2 M H 2 SO 4 was used, the extraction solution was adjusted to pH 4 with 1 M NaOH in water and then the mixture was centrifuged at 6000 rpm (68426g) for 20 min.…”

Section: Extraction Of Av From Animal Tissuesmentioning

confidence: 99%

“…Kadota et al [5,6] published a procedure for detecting AV in chicken muscle using SPE and LC with UV and amperometric detection. The detection limit of AV in chicken muscle is 0.2 lg/g (0.2 ppm) using LC/ amperometric detection [6].…”

Section: Introductionmentioning

confidence: 99%

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Determination of avoparcin in animal tissues and milk using LC‐ESI‐MS/MS and tandem‐SPE

Inoue

Mizuno

Yoshimi

et al. 2008

J of Separation Science

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A highly sensitive and selective method using LC-ESI-MS/MS and tandem-SPE was developed to detect trace amounts of avoparcin (AV) antibiotics in animal tissues and milk. Data acquisition using MS/MS was achieved by applying multiple reaction monitoring of the product ions of [M + 3H](3+) and the major product ions of AV-alpha and -beta at m/z 637 --> 86/113/130 and m/z 649 --> 86/113/130 in ESI(+) mode. The calculated instrumental LODs were 3 ng/mL. The sample preparation was described that the extraction using 5% TFA and the tandem-SPE with an ion-exchange (SAX) and InertSep C18-A cartridge clean-up enable us to determine AV in samples. Ion suppression was decreased by concentration rates of each sample solution. These SPE concentration levels could be used to detect quantities of 5 ppb (milk), 10 ppb (beef), and 25 ppb (chicken muscle and liver). The matrix matching calibration graphs obtained for both AV-alpha (r >0.996) and -beta (r >0.998) from animal tissues and milk were linear over the calibration ranges. AV recovery from samples was higher than 73.3% and the RSD was less than 12.0% (n = 5).

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Section: Lc-ms/ms Detection Of Av-a and -Bmentioning

confidence: 99%

Section: Investigation Of Sample Preparation Of Avmentioning

confidence: 99%

Section: Extraction Of Av From Animal Tissuesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Determination of avoparcin in animal tissues and milk using LC‐ESI‐MS/MS and tandem‐SPE

Inoue

Mizuno

Yoshimi

et al. 2008

J of Separation Science

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“…This result is in agreement with previous results found in the literature (the ratio of ␣to ␤-avoparcin being 1:3 or 1:4). [71][72][73][74][75] The avoparcin mixture is soluble in water (with maximum stability in aqueous buffer solution at pH 4-8), 76 dimethylformamide (DMF), and dimethylsulfoxide (DMSO). 77 It is slightly soluble in methanol (MeOH).…”

mentioning

confidence: 99%

Evaluation of the macrocyclic antibiotic avoparcin as a new chiral selector for HPLC

et al. 1998

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Avoparcin is a macrocyclic glycopeptide antibiotic structurally related to vancomycin, teicoplanin, and ristocetin A. When attached to 5‐μm spherical silica gel, the avoparcin proved to be an effective chiral stationary phase (CSP) that could be used in the reversed‐phase, normal‐phase, and polar‐organic modes. The avoparcin CSP was complimentary to the other macrocyclic glycopeptide CSPs in that it could resolve some racemates that the others could not, and vice versa. Some important compounds resolved on the avoparcin CSP include verapamil, thyroxine, mephenytoin, and 2‐imidazolidone‐4‐carboxylic acid. The use of this CSP and the optimization of separations on it are discussed. Avoparcin appears to be a useful addition to this family of CSPs. Chirality 10:627–660, 1998. © 1998 Wiley‐Liss, Inc.

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Avoparcin, a new macrocyclic antibiotic chiral run buffer additive for capillary electrophoresis

Ekborg-Ott¹,

Zientara²,

Schneiderheinze³

et al. 1999

Electrophoresis

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Determination of Residual Avoparcin in Chicken Muscle by High Performance Liquid Chromatography

Cited by 5 publications

References 0 publications

Determination of avoparcin in animal tissues and milk using LC‐ESI‐MS/MS and tandem‐SPE

Determination of avoparcin in animal tissues and milk using LC‐ESI‐MS/MS and tandem‐SPE

Evaluation of the macrocyclic antibiotic avoparcin as a new chiral selector for HPLC

Avoparcin, a new macrocyclic antibiotic chiral run buffer additive for capillary electrophoresis

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