Determination of remifentanil in human plasma by liquid chromatography–tandem mass spectrometry utilizing micro extraction in packed syringe (MEPS) as sample preparation

Said, Rana; Pohanka, Anton; Andersson, Maria; Beck, Olof; Abdel‐Rehim, Mohamed

doi:10.1016/j.jchromb.2011.02.002

Cited by 53 publications

(25 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The MEPS technique has been used to extract a wide range of analytes. Thus, several drugs such as local anaesthetics and their metabolites 16–19; UV filter and polycyclic musk 20, antipsychotic drug risperidone 21, non‐polar heterocyclic amines 22, estrogenic compounds 23, anticancer drugs roscovitine, olomoucine, cyclophosphamide and busulfan 24, 25, atorvastatin and its metabolites 26, anti‐depressant drugs dopamine and serotonine 27; and opoid analgesic drug remifentanil 28 have been extracted successfully from biological samples such as plasma, urine or blood. In addition, polycyclic aromatic hydrocarbons in water have been extracted with MEPS 29.…”

Section: Introductionmentioning

confidence: 99%

Novel micro‐extraction by packed sorbent procedure for the liquid chromatographic analysis of antiepileptic drugs in human plasma and urine

Rani

Malik

Singh

2012

J of Separation Science

View full text Add to dashboard Cite

A method for the simultaneous determination of the antiepileptic drugs, phenobarbital (PHB), phenytoin (PTN), carbamazepine (CBZ), primidone (PRM) and oxcarbazepine (OXC) in human plasma and urine samples by using micro-extraction in a packed syringe as the sample preparation method connected with LC/UV (MEPS/LC/UV) is described. Micro-extraction in a packed syringe (MEPS) is a new miniaturized, solid-phase extraction technique that can be connected online to gas or liquid chromatography without any modifications. In MEPS approximately 1 mg of the solid packing material is inserted into a syringe (100-250 μL) as a plug. Sample preparation takes place on the packed bed. The bed can be coated to provide selective and suitable sampling conditions. The new method is very promising, easy to use, fully automated, inexpensive and quick. The standard curves were obtained within the concentration range 1-500 ng/mL in both plasma and urine samples. The results showed high correlation coefficients (R(2) >0.988) for all of the analytes within the calibration range. The extraction recovery was found to be between 88.56 and 99.38%. The limit of quantification was found to be between 0.132 and 1.956 ng/mL. The precision (RSD) values of quality control samples (QC) had a maximum deviation of 4.9%. A comparison of the detection limits with similar methods indicates high sensitivity of the present method. The method is applied for the analysis of these drugs in real urine and plasma samples of epileptic patients.

show abstract

Section: Introductionmentioning

confidence: 99%

Novel micro‐extraction by packed sorbent procedure for the liquid chromatographic analysis of antiepileptic drugs in human plasma and urine

Rani

Malik

Singh

2012

J of Separation Science

View full text Add to dashboard Cite

show abstract

“…Shell particles are a relatively recent trend in chromatographic separation, but several pharmaceutical-bioanalytical [105][106][107][108][109][110][111][112][113][114][115][116][117][118], food analytical [119][120][121][122][123][124][125][126][127][128][129], environmental [130][131][132][133][134] and multidimensional [135][136][137] separations can be found in the literature. Very recently, new commercially available wide-pore stationary phases demonstrate exceptional efficiency for protein separations [138][139][140].…”

Section: Superficially Porous Particlesmentioning

confidence: 99%

Evolution and Current Trends in Liquid and Supercritical Fluid Chromatography

Fekete¹,

Perrenoud²,

Guillarme

2014

CCHG

View full text Add to dashboard Cite

Abstract:The current trend in high performance liquid chromatography (HPLC) tends toward the achievement of higher separation efficiency and shorter analysis time. Indeed, better performance in LC has become increasingly important in recent years mainly driven by the challenges of either analyzing more complex samples or increasing the numbers of samples per time unit. In the recent development of particle technology, the use of fully porous sub-2 m particles and sub-3 m shell particles have received considerable attention. Beside packed columns, the new generation of silica-based monolithic columns also offers very high separation power. However, to take full advantage of these innovative phases, the chromatographic system has also to be drastically optimized in terms of upper pressure limit and system volume.This revolution in column technology now spreads and covers several modes of liquid chromatography such as reversedphase liquid chromatography (RPLC), hydrophilic interaction liquid chromatography (HILIC), or even supercritical fluid chromatography (SFC). The HILIC and SFC, which can be considered as alternative modes of chromatography, could also be useful to extend the applicability of chromatography towards the analysis of very hydrophilic and lipophilic compounds, respectively.The present review gives an insight about the theory behind the success of current column technology and presents a summary of latest applications, using various modes of one-dimensional chromatography (RPLC, HILIC, SFC). This paper also shows that theoretically expected column efficiency could sometimes be compromised in practical work especially in the case of narrow bore columns.

show abstract

“…MEPS is practical for small volumes of sample (10 μL) and can handle volumes up to 1000 μL. MEPS has also been utilized to the extraction of drugs and metabolites from plasma Said et al, 2011;Vita et al, 2005;Ashri et al, 2013), urine El-Beqqali et al, 2007b;Abdel-Rehim et al, 2006, saliva (Abdel-Rehim and Abdel-Rehim, 2013) and blood samples (Said et al, 2010(Said et al, , 2012. MEPS has been reported in a large number of research works showing MEPS's ability to provide an improved sample preparation, particularly aiding the speed, sensitivity and selectivity options provided by LC-MS/MS (Abdel-Rehim, Abdel-Rehim, 2009a, 2009b.…”

Section: Introductionmentioning

confidence: 99%

Determination of metoprolol enantiomers in human plasma and saliva samples utilizing microextraction by packed sorbent and liquid chromatography–tandem mass spectrometry

Elmongy

Ahmed

Wahbi

et al. 2016

Biomedical Chromatography

Self Cite

View full text Add to dashboard Cite

A sensitive, accurate and reliable bioanalytical method for the enantioselective determination of metoprolol in plasma and saliva samples utilizing liquid chromatography-electrospray ionization tandem mass spectrometry was developed and validated. Human plasma and saliva samples were pretreated by microextraction by packed sorbent (MEPS) prior to analysis. A new MEPS syringe form with two inputs was used. Metoprolol enantiomers and internal standard pentycaine (IS) were eluted from MEPS sorbent using isopropanol after removal of matrix interferences using aliquots of 5% methanol in water. Complete separation of metoprolol enantiomers was achieved on a Cellulose-SB column (150 × 4.6 mm, 5 μm) using isocratic elution with mobile phase 0.1% ammonium hydroxide in hexane-isopropanol (80:20, v/v) with a flow rate of 0.8 mL/min. A post-column solvent-assisted ionization was applied to enhance metoprolol ionization signal in positive mode monitoring (+ES) using 0.5% formic acid in isopropanol at a flow rate of 0.2 mL/min. The total chromatographic run time was 10 min for each injection. The detection of metoprolol in plasma and saliva samples was performed using triple quadrupole tandem mass spectrometer in +ES under the following mass transitions: m/z 268.08 → 72.09 for metoprolol and m/z 303.3 → 154.3 for IS. The linearity range was 2.5-500 ng/mL for both R- and S-metoprolol in plasma and saliva. The limits of detection and quantitation for both enantiomers were 0.5 and 2.5 ng/mL respectively, in both matrices (plasma and saliva). The intra- and inter-day precisions were presented in terms of RSD values for replicate analysis of quality control samples and were <5%; the accuracy of determinations varied from 96 to 99%. The method was able to determine the therapeutic levels of metoprolol enantiomers in both human plasma and saliva samples successfully, which can aid in therapeutic drug monitoring in clinical laboratories. Copyright © 2016 John Wiley & Sons, Ltd.

show abstract

Determination of remifentanil in human plasma by liquid chromatography–tandem mass spectrometry utilizing micro extraction in packed syringe (MEPS) as sample preparation

Cited by 53 publications

References 20 publications

Novel micro‐extraction by packed sorbent procedure for the liquid chromatographic analysis of antiepileptic drugs in human plasma and urine

Novel micro‐extraction by packed sorbent procedure for the liquid chromatographic analysis of antiepileptic drugs in human plasma and urine

Evolution and Current Trends in Liquid and Supercritical Fluid Chromatography

Determination of metoprolol enantiomers in human plasma and saliva samples utilizing microextraction by packed sorbent and liquid chromatography–tandem mass spectrometry

Contact Info

Product

Resources

About