Determination of reliable lung function parameters in intubated mice

Bonnardel, Éline; Prével, Renaud; Campagnac, Marilyne; Dubreuil, Marielle; Marthan, Roger; Berger, Patrick; Dupin, Isabelle

doi:10.1186/s12931-019-1177-9

Cited by 27 publications

(13 citation statements)

References 29 publications

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“…Furthermore, ventilatory parameters such as forced vital capacity (FVC), forced expiratory volume in the first 0.1 second (FEV 0.1 ) and compliance of respiratory system (C rs ) [ 27 ] provide relevant information about the lung function; however, the longitudinal application of this method on a large number of animals is quite challenging since mice need to be intubated and data could be affected by technical procedure.…”

Section: Discussionmentioning

confidence: 99%

Pivotal role of micro-CT technology in setting up an optimized lung fibrosis mouse model for drug screening

Khalajzeyqami

Grandi²,

Ferrini³

et al. 2022

PLoS ONE

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Idiopathic pulmonary fibrosis (IPF) is a progressive disease with no curative pharmacological treatment. The most used animal model of IPF for anti-fibrotic drug screening is bleomycin (BLM)-induced lung fibrosis. However, several issues have been reported: the balance among disease resolution, an appropriate time window for therapeutic intervention and animal welfare remain critical aspects yet to be fully elucidated. In this study, C57Bl/6 male mice were treated with BLM via oropharyngeal aspiration (OA) following either double or triple administration. The fibrosis progression was longitudinally assessed by micro-CT every 7 days for 4 weeks after BLM administration. Quantitative micro-CT measurements highlighted that triple BLM administration was the ideal dose regimen to provoke sustained lung fibrosis up to 28 days. These results were corroborated with lung histology and Bronchoalveolar Lavage Fluid cells. We have developed a mouse model with prolonged lung fibrosis enabling three weeks of a curative therapeutic window for the screening of putative anti-fibrotic drugs. Moreover, we have demonstrated the pivotal role of longitudinal micro-CT imaging in reducing the number of animals required per experiment in which each animal can be its own control. This approach permits a valuable decrease in costs and time to develop disease animal models.

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Section: Discussionmentioning

confidence: 99%

Pivotal role of micro-CT technology in setting up an optimized lung fibrosis mouse model for drug screening

Khalajzeyqami

Grandi²,

Ferrini³

et al. 2022

PLoS ONE

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“…This perturbation then plotted flow–volume curves and resultant forced expiratory volume at 0.1 seconds (FEV 0.1 ), forced vital capacity (FVC) and the ratio between the two (FEV0.1/FVC). FEV 0.1 is utilized in mouse models as analogous to FEV 1 in human spirometry (Bonnardel et al, 2019 ).…”

Section: Methodsmentioning

confidence: 99%

Haploinsufficiency of Col5a1 causes intrinsic lung and respiratory changes in a mouse model of classical Ehlers‐Danlos syndrome

Fett

Dimori

Carroll

et al. 2022

Physiological Reports

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The Ehlers-Danlos syndromes (EDS) represent a genetically and clinically heterogeneous group of inherited connective tissue diseases (for a review see Malfait et al., 2020) that share clinical features including joint hypermobility, tissue fragility, and skin changes with a spectrum of severity that goes from subclinical to life-threatening. The 2017 international Ehlers-Danlos syndrome classification describes 13 different types of EDS and a fourteenth rare subtype has recently been identified (Blackburn et al., 2018;Malfait et al., 2017). Variants in 20 distinct genes can cause

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“…The primary drawback of the invasive techniques involving tracheostomy is the inability to make repeated measurements. This limitation has been overcome by the use of an orotracheal intubation technique, allowing for repetitive monitoring of airway mechanics in the same animals [ 12 , 13 ]. However, this modification still requires anesthesia and is technically challenging.…”

Section: Lung Function Measurements In Mice: Strengths and Limitationsmentioning

confidence: 99%

Noninvasive Measurement of Pulmonary Function in Experimental Mouse Models of Airway Disease

Glaab

Braun

2021

Lung

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Mouse models have become an indispensable tool in translational research of human airway disease and have provided much of our understanding of the pathogenesis of airway disease such as asthma. In these models the ability to assess pulmonary function and particularly airway responsiveness is critically important. Existing methods for testing pulmonary function in mice in vivo include noninvasive and invasive technologies. Noninvasive head-out body plethysmography is a well-established and widely accepted technique which has been proven as a reliable method to measure lung function on repeated occasions in intact, conscious mice. We have performed several validation studies in allergic mice to compare the parameter midexpiratory flow (EF50) as a noninvasive marker of airflow limitation with invasively measured gold standard parameters of lung mechanics. The results of these studies showed a good agreement of EF50 with the invasive assessment of lung resistance and dynamic compliance with a somewhat lower sensitivity of EF50. The measurement of EF50 together with basic respiratory parameters is particularly appropriate for simple and repeatable screening of pulmonary function in large numbers of mice or if noninvasive measurement without use of anesthesia is required. Beyond known applications, head-out body plethysmography also provides a much-needed high-throughput screening tool to gain insights into the impact and kinetics of respiratory infections such as SARS-COV-2 on lung physiology in laboratory mice.

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Determination of reliable lung function parameters in intubated mice

Cited by 27 publications

References 29 publications

Pivotal role of micro-CT technology in setting up an optimized lung fibrosis mouse model for drug screening

Pivotal role of micro-CT technology in setting up an optimized lung fibrosis mouse model for drug screening

Haploinsufficiency of Col5a1 causes intrinsic lung and respiratory changes in a mouse model of classical Ehlers‐Danlos syndrome

Noninvasive Measurement of Pulmonary Function in Experimental Mouse Models of Airway Disease

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