Determination of relative assay response factors for toxic chlorinated and brominated dioxins/furans using an enzyme immunoassay (EIA) and a chemically-activated luciferase gene expression cell bioassay (CALUX)

Samara, Fatin; Gullett, Brian K.; Harrison, Robert O.; Chu, Andrew Chi-Yuen; Clark, George C.

doi:10.1016/j.envint.2008.11.003

Cited by 71 publications

(50 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The elevated BEQ activities of each of the first test set of samples (83,102,112,113,118), relative to the GC/HRMS TEQ values, suggests that these extracts contain AhR agonists in addition to the PCDD/Fs. Potential chemicals could include brominated- or mixed chloro/bromo-dibenzo-p-dioxins, dibenzofurans, chloronaphthalenes and others that have been shown to be AhR active [31,32,39–42], but are not currently measured using standard GC/HRMS analysis and currently do not have TEF values.…”

Section: Resultsmentioning

confidence: 99%

Considerations for potency equivalent calculations in the Ah receptor-based CALUX bioassay: Normalization of superinduction results for improved sample potency estimation

Baston

Denison

2011

Talanta

View full text Add to dashboard Cite

The chemically activated luciferase expression (CALUX) system is a mechanistically based recombinant luciferase reporter gene cell bioassay used in combination with chemical extraction and clean-up methods for the detection and relative quantitation of 2,3,7,8-tetrachlorodibenzo-pdioxin and related dioxin-like halogenated aromatic hydrocarbons in a wide variety of sample matrices. While sample extracts containing complex mixtures of chemicals can produce a variety of distinct concentration-dependent luciferase induction responses in CALUX cells, these effects are produced through a common mechanism of action (i.e. the Ah receptor (AhR)) allowing normalization of results and sample potency determination. Here we describe the diversity in CALUX response to PCDD/Fs from sediment and soil extracts and not only report the occurrence of superinduction of the CALUX bioassay, but we describe a mechanistically based approach for normalization of superinduction data that results in a more accurate estimation of the relative potency of such sample extracts.

show abstract

Section: Resultsmentioning

confidence: 99%

Considerations for potency equivalent calculations in the Ah receptor-based CALUX bioassay: Normalization of superinduction results for improved sample potency estimation

Baston

Denison

2011

Talanta

View full text Add to dashboard Cite

show abstract

“…The structural similarity between both polybrominated dibenzop-dioxins/dibenzofurans (PBDD/Fs) and PCDD/Fs results in the similarity of mechanisms of formation of these compounds during thermal treatments in de novo synthesis and/or the thermolysis of precursor compounds (such as brominated aromatic compounds) (Weber and Kuch, 2003). The toxicity of PBDD/Fs is comparable to that of their chlorinated analogs (Wilken et al, 1990;Weber and Greim, 1997;Behnisch et al, 2003;Samara et al, 2009), so PBDD/Fs and other BFRs have become serious environmental concerns in the past decade.…”

Section: Introductionmentioning

confidence: 89%

Characterization of Persistent Organic Pollutants Emitted from a Municipal Solid Waste Incinerator in Taiwan

Wang

Chen

Huang

et al. 2010

Environmental Engineering Science

View full text Add to dashboard Cite

No information is currently available on the emission of brominated flame retardant (BFR) compounds from stack flue gases of municipal solid waste incinerators (MSWIs) because of the limitations of standard methods of BFR sampling and analysis. This study presents sample cleanup procedures that are combined with a multiple column approach to determine the quantities of five persistent organic pollutants (polychlorinated dibenzo-p-dioxins/ dibenzofurans [PCDD/Fs], polybrominated dibenzo-p-dioxins/dibenzofurans [PBDD/Fs], polybrominated diphenyl ethers [PBDEs], polychlorinated biphenyls [PCBs], and polybrominated biphenyls [PBBs]) simultaneously from single stack gas samples collected from a large-scale MSWI in Taiwan. Mean concentrations of PCDD/Fs, PBDD/Fs, PCBs, PBDEs, and PBBs in the flue gases of MSWI were 0.0719 ng WHO-TEQ/(N Á m 3 ), 0.00169 ng WHO-TEQ/(N Á m 3 ), 0.00546 ng WHO-TEQ/(N Á m 3 ), 20.7 ng/(N Á m 3 ), and 0.958 ng/( N Á m 3 ), respectively. Emission factors of PCDD/Fs, PBDD/Fs, PCBs, PBDEs, and PBBs were 0.300 mg WHO-TEQ/ton-waste, 0.00667 mg WHO-TEQ/ton-waste, 0.0207 mg WHO-TEQ/ton-waste, 84.5 mg/ton-waste, and 1.05 mg/ton-waste, respectively. Therefore, the emissions of not only PCDD/Fs but also BFRs from MSWIs should be of concern and further investigation is needed.

show abstract

“…When using these CALUX-REP values, a CALUX/GC-HRMS ratio of 1.39 was obtained for the dl-PCBs (Table 5). This small overestimation could be due to the slight differences between REP values of the H1L6.1 and H1L7.5 cell lines (resulting from enhanced sensitivity of the H1L7.5 cells), the uncertainty of the CALUX and GC-HRMS measurements, the uncertainty of the CALUX-REP values (various other reported slightly different REP values for the same cell line) [31, 32] and/or to the presence of other AhR agonists in the extract (e.g. brominated compounds for which no REP values are yet established).…”

Section: Resultsmentioning

confidence: 99%

Determination of PCDD/Fs, PBDD/Fs and dioxin-like PCBs in human milk from mothers residing in the rural areas in Flanders, using the CALUX bioassay and GC-HRMS

Croes

Colles

Koppen

et al. 2013

Talanta

View full text Add to dashboard Cite

Since the CALUX (Chemically Activated LUciferase gene eXpression) bioassay is a fast and inexpensive tool for the determination of dioxin-like compounds in a large number of samples and requires only small sample volumes, the use of this technique in human biomonitoring programs provides a good alternative to GC-HRMS. In this study, a new CALUX method for the separate analysis of PCDD/Fs and dioxin-like PCBs (dl-PCBs) in small amounts of human milk samples with the new sensitive H1L7.5c1 cell line was used to analyze 84 human milk samples, collected from mothers residing in the Flemish rural communities. The geometric mean CALUX-Bioanalytical Equivalent (CALUX-BEQ) values, reported for the 84 mothers from the study area were 10.4 (95% CI: 9.4–11.4) pg CALUX-BEQ per g lipid or 0.41 (95% CI: 0.37–0.45) pg CALUX-BEQ per g milk for the PCDD/Fs and 1.73 (1.57–1.91) pg CALUX-BEQ per g lipid or 0.07 (95% CI: 0.06–0.08) pg CALUX-BEQ per g milk for the dioxin-like PCBs. Multiple regression analysis showed significant associations between PCDD/Fs and weight change after pregnancy, smoking and consumption of local eggs. One pooled human milk sample was analyzed with both CALUX and GC-HRMS. The ratio of CALUX and GC-HRMS results for this sample were respectively 1.60, 0.58 and 1.23 for the PCDD/Fs, the dl-PCBs and the sum of both fractions, when using the 2005-TEF values. Additionally, also low levels of certain brominated dioxins and furans were detected in the pooled sample with GC-HRMS.

show abstract

Determination of relative assay response factors for toxic chlorinated and brominated dioxins/furans using an enzyme immunoassay (EIA) and a chemically-activated luciferase gene expression cell bioassay (CALUX)

Cited by 71 publications

References 24 publications

Considerations for potency equivalent calculations in the Ah receptor-based CALUX bioassay: Normalization of superinduction results for improved sample potency estimation

Considerations for potency equivalent calculations in the Ah receptor-based CALUX bioassay: Normalization of superinduction results for improved sample potency estimation

Characterization of Persistent Organic Pollutants Emitted from a Municipal Solid Waste Incinerator in Taiwan

Determination of PCDD/Fs, PBDD/Fs and dioxin-like PCBs in human milk from mothers residing in the rural areas in Flanders, using the CALUX bioassay and GC-HRMS

Contact Info

Product

Resources

About