Determination of reference intervals for urinary steroid profiling using a newly validated GC-MS/MS method

Jong, Wilhelmina H. A. de; Buitenwerf, Edward; Pranger, Alle; Riphagen, Ineke J.; Wolffenbuttel, Bruce H. R.; Kerstens, Michiel N.; Kema, Ido P.

doi:10.1515/cclm-2016-1072

Cited by 25 publications

(22 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…de Jong et al [ 58 ] recently used GC/MS/MS for urinary targeted steroidomics and determined the reference intervals for most of the steroid metabolites commonly measured (those shown in Fig. 1 and others) for males and females.…”

Section: Resultsmentioning

confidence: 99%

“…Further normative data of 67 components of the neonatal metabolome (to 1 year of life) has been reported by Dhayat et al [ 73 ], who also reported studies discussing the influence of the alternative androgen pathway in young infants [ 74 ]. Normative data (obtained by GC/MS/MS) for the full age range from newborn to adult was reported by de Jong et al [ 58 ]. All these investigators essentially used the common MO-TMS derivative method.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

GC/MS in Recent Years Has Defined the Normal and Clinically Disordered Steroidome: Will It Soon Be Surpassed by LC/Tandem MS in This Role?

Shackleton

Pozo

Marcos

2018

Journal of the Endocrine Society

View full text Add to dashboard Cite

Gas chromatography/mass spectrometry (GC/MS) has been used for steroid analysis since the 1960s. The advent of protective derivatization, capillary columns, and inexpensive electron ionization bench-top single quadrupole soon made it the method of choice for studying disorders of steroid synthesis and metabolism. However, the lengthy sample workup prevented GC/MS from becoming routine for steroid hormone measurement, which was dominated by radioimmunoassay. It was the emergence of liquid chromatography/tandem MS (LC/MS/MS) that sparked a renewed interest in GC/MS for the multicomponent analysis of steroids. GC/MS is excellent at providing an integrated picture of a person's steroid metabolome, or steroidome, as we term it. We review the recent work on newly described disorders and discuss the technical advances such as GC coupling to triple quadrupole and ion trap analyzers, two-dimensional GC/MS, and alternative ionization and detection systems such as atmospheric pressure chemical ionization (APCI) and time of flight. We believe that no novel GC/MS-based technique has the power of GC(electron ionization)/MS/MS as a “discovery tool,” although APCI might provide ultimate sensitivity, which might be required in tissue steroidomics. Finally, we discuss the role of LC/MS/MS in steroidomics. This remains a challenge but offers shorter analysis times and advantages in the detection and discovery of steroids with a known structure. We describe recent advances in LC/MS steroidomics of hydrolyzed and intact steroid conjugates and suggest the technique is catching up with GC/MS in this area. However, in the end, both techniques will likely remain complementary and both should be available in advanced analytical laboratories.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

GC/MS in Recent Years Has Defined the Normal and Clinically Disordered Steroidome: Will It Soon Be Surpassed by LC/Tandem MS in This Role?

Shackleton

Pozo

Marcos

2018

Journal of the Endocrine Society

View full text Add to dashboard Cite

show abstract

“…We used LC‐MS/MS to determine the main metabolites of cortisol and cortisone where the gold standard to measure these metabolites is GC‐MS . However, our assay is very well developed and validated . Therefore, we do not think that this will influence our results.…”

Section: Discussionmentioning

confidence: 99%

“…Other metabolites include α/β‐cortol, α/β‐cortolone, and 1β‐hydroxyetiocholanolone. However, THF, 5a‐THF, and THE account for almost 80% of the metabolites of cortisol and cortisone …”

Section: Discussionmentioning

confidence: 99%

“…Total urinary cortisol, cortisone, THF, alloTHF, and THE were measured by using a validated high‐performance liquid chromatography tandem‐mass spectrometry (LC‐MS/MS) assay . For THE, THF, and a‐THF, we compared the LC‐MS/MS method with a GC‐MS/MS method and found the following correlations: THE r 2 = .99, THF r 2 = .97, and a‐THF r 2 = .94 for 40 urines …”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Endogenous urinary glucocorticoid metabolites and mortality in prednisolone‐treated renal transplant recipients

Vulto

Minovíc

Vries

et al. 2020

Clinical Transplantation

Self Cite

View full text Add to dashboard Cite

Background: Chronic corticosteroid treatment suppresses HPA-axis activity and might alter activity of 11β hydroxysteroid dehydrogenases (11β-HSD). We aimed to investigate whether the endogenous glucocorticoid production and 11β-HSD activities are altered in prednisolone-treated renal transplant recipients (RTR) compared with healthy controls and whether this has implications for long-term survival in RTR. Methods: In a longitudinal cohort of 693 stable RTR and 275 healthy controls, 24-hour urinary cortisol, cortisone, tetrahydrocorisol (THF), allotetrahydrocortisol (alloTHF), and tetrahydrocortisone (THE) were measured using liquid chromatography tandemmass spectrometry. Twenty-four-hour urinary excretion of cortisol and metabolites were used as measures of endogenous glucocorticoid production; (THF + alloTHF)/ THE and cortisol/cortisone ratios were used as measures of 11β-HSD activity.Results: Urinary cortisol and metabolite excretion were significantly lower in RTR compared with healthy controls (P < .001), whereas (THF + alloTHF)/THE and cortisol/cortisone ratios were significantly higher (P < .001 and P = .002). Lower total urinary metabolite excretion and higher urinary (THF + alloTHF)/THE ratios were associated with increased risk of mortality, independent of age, sex, estimated glomerular filtration rate, C-reactive protein, body surface area, and daily prednisolone dose, respectively. Conclusions: Endogenous glucocorticoid production and 11β-HSD activities are altered in prednisolone-treated RTR. Decreased total urinary endogenous glucocorticoid metabolite excretion and increased urinary (THF + alloTHF)/THE ratios are associated with increased risk of mortality. K E Y W O R D S 11βHSD, cortisol, mortality, prednisolone, renal transplant recipients S U PP O RTI N G I N FO R M ATI O N Additional supporting information may be found online in the Supporting Information section. How to cite this article: Vulto A, Minović I, de Vries LV, et al. Endogenous urinary glucocorticoid metabolites and mortality in prednisolone-treated renal transplant recipients. Clin

show abstract

Steroids in urine, other fluids and tissues

Honour

2023

Steroids in the Laboratory and Clinical Practice

View full text Add to dashboard Cite

Determination of reference intervals for urinary steroid profiling using a newly validated GC-MS/MS method

Cited by 25 publications

References 24 publications

GC/MS in Recent Years Has Defined the Normal and Clinically Disordered Steroidome: Will It Soon Be Surpassed by LC/Tandem MS in This Role?

GC/MS in Recent Years Has Defined the Normal and Clinically Disordered Steroidome: Will It Soon Be Surpassed by LC/Tandem MS in This Role?

Endogenous urinary glucocorticoid metabolites and mortality in prednisolone‐treated renal transplant recipients

Steroids in urine, other fluids and tissues

Contact Info

Product

Resources

About