Determination of reactogenicity and allergenicity of the immunobiological drug for prevention and treatment of candidiasis

Rybalkin, M. V.; Філімонова, Н. І.; Strilets, O. P.; Стрельніков, Л. С.

doi:10.24959/nphj.14.1991

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“…In recent years, several research groups around the world have focused on the creation of a vaccine against candidiasis (Zaharieva et al, 2019;Edwards, 2012;Rybalkin et al, 2014), one of the most severe and deadly fungal infections.…”

Section: Introductionmentioning

confidence: 99%

Research of antibody titres to antigens of a low molecular fraction of C. Albicans fungus disintegrate at preventing candidomicosis

Rybalkin

Diadiun

et al. 2020

ijrps

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The purpose of this work is the study of antibody titers of low molecular weight proteins fractions less than ten kDa with C. Albicans protein concentrations of 1, 2, 3 and 4 mg/ml in the prevention of candidiasis. A low molecular fraction of C. Albicans proteins in concentration 1, 2, 3, 4 and 5 mg/ml were examined in white mice. The mice were intramuscularly injected into the upper part of the right hind paw 0.2 ml of the test fractions with the investigated protein concentrations. After 14 days, 0.2 ml of test fractions were injected again into the upper part of the left hind paw. Animals in the control group were administered saline. After one month for one group and after three months for the second group of experimental animals after the second injection, intraperitoneal infection of the animals was carried out. For this purpose, a suspension of C. Albicans fungi was used in the amount of 20 million cells in a volume of 1 ml. After 14 days, determination of the protective functions of the animal body by the titer of specific C. Albicans antibodies has been performed during enzyme-linked immunoassay. Studies have shown that the low molecular weight fraction of C. Albicans fungi cells antigens with protein concentrations of 1, 2, 3 and 4 mg/ml at a double intramuscular injection of 0.2 ml does not provide activation of immune mechanisms. The low molecular weight fraction of C. Albicans proteins does not activate the body's defence mechanisms.

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Section: Introductionmentioning

confidence: 99%