Determination of rat heart morphology and function in vivo in two models of cardiac hypertrophy by means of magnetic resonance imaging

Rudin, Markus; Pedersen, B; Umemura, Kazuo; Zierhut, Wolfgang

doi:10.1007/bf02190549

Cited by 46 publications

(23 citation statements)

References 13 publications

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“…Respiration and ECG were monitored continuously. MRI experiments were performed as described earlier (Rudin et al 1991). The images were acquired by a spinecho sequence SE (500/20), the eld of view was 50 mm, the image resolution was 256 3 256 pixels with a dimension of 0.2 3 0.2 mm.…”

Section: Magnetic Resonance Imagingmentioning

confidence: 99%

“…Before the acquisition of data, a coronary pilot scan was measured for adequate positioning of the transverse slices. MRI data acquisition was gated to the cardiac cycle by a Physiograd SM 785 MR monitoring system (Bruker, Karlsruhe, Germany) as previously described (Rudin et al 1991). Two sets of transverse images were acquired, one at end-systole and another at end-diastole.…”

Section: Magnetic Resonance Imagingmentioning

confidence: 99%

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A model of chronic heart failure in spontaneous hypertensive rats (SHR)

Itter¹,

Jung²,

Juretschke³

et al. 2004

Lab Anim

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Chronic heart failure (CHF) is one of the most common causes of death in Western countries. The aim of this study was to establish and validate a model of CHF in the rat. This rat model should result in parameters and symptoms that can be extrapolated to the clinical situation of patients with end-stage heart failure. At present only palliative therapy is possible for patients with heart failure but the aim for the future is to nd a causal therapy of heart failure treatment. The rat model should be a valuable method for the early testing of new therapeutic approaches in patients with congestive heart failure. SummaryCommon models of chronic heart failure (CHF) do not always result in parameters and symptoms that can be extrapolated to the clinical situation of patients with end-stage heart failure. The aim of this study was to establish and validate a new model of CHF in the rat. CHF was induced in Wistar Kyoto (WKY/NHsd) and spontaneously hypertensive (SHR/NHsd) rats by creating a permanent (8-week) occlusion of the left coronary artery 2 mm distal to the origin from the aorta by a modi ed technique. This resulted in a large infarction of the free left ventricular wall. The focus of attention was the validation of the geometric properties of the left ventricle and its contractility. The validation of the geometric properties of the left ventricle was done by a non-invasive magnetic resonance imaging (MRI) technique and by planimetry (stereology). Cardiodynamics (e.g. contractility) were evaluated in the isolated 'working heart' model. We were able to establish a new and predictive model of heart failure in the spontaneously hypertensive rat 8 weeks after coronary artery ligation. At this time point, the WKY rat did not show any symptoms of CHF. The model represents characteristic parameters and symptoms that can be extrapolated to the clinical situation of patients with end-stage heart failure (NYHA III-IV). Upon inspection, severe clinical symptoms of congestive heart failure were prominent, such as dyspnoea, subcutaneous oedema, pale-bluish limbs and impaired motion. Non-invasive sequential measurements by NMR techniques showed lung oedema, hydrothorax, large dilated left and right ventricular chambers and hypertrophy of the septum. The infarcted animals showed a reduced heart power, diminished contractility and enhanced heart work, much more so in the SHR/NHsd rat than in the WKY/NHsd rat. Furthermore the infarcted animals showed enhanced levels of hydroxyproline/proline ratios, again much more so in the SHR/NHsd rat than in the WKY/NHsd rat.

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Section: Magnetic Resonance Imagingmentioning

confidence: 99%

Section: Magnetic Resonance Imagingmentioning

confidence: 99%

A model of chronic heart failure in spontaneous hypertensive rats (SHR)

Itter¹,

Jung²,

Juretschke³

et al. 2004

Lab Anim

View full text Add to dashboard Cite

show abstract

“…There has been increasing interest in the investigation of animal models of cardiac pathology in small mammals using MRI techniques in combination with conventional analytical and histological investigations (Manning et al 1990;Rudin et al 1991;Rose et al 1994;Laurent et al 1995;Rehwald et al 1997;Franco et al 1997;Lorenz et al 1997;Slawson et al 1997). Some of those studies examine functional parameters of the left ventricle; typically images are acquired in synchrony with the R wave of an electrocardiographic (ECG) signal.…”

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confidence: 99%

Magnetic resonance imaging analysis of left ventricular function in normal and spontaneously hypertensive rats

Wise

Huang

Gresham

et al. 1998

The Journal of Physiology

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We have used magnetic resonance imaging (MRI) to examine acute morphological changes in the left ventricle throughout the cardiac cycle in normal Wistar Kyoto rats (WKY) and also to follow the development of chronic changes in spontaneously hypertensive rats (SHR). This involved the development of MRI and quantitative analysis techniques for characterizing contractile changes during the cardiac cycle. Images of the cardiac anatomy in two age groups (8 and 12 weeks old) of young anaesthetized adult normal WKY and SHR were acquired in planes both parallel and perpendicular to the principal cardiac axis. Complete coverage of the heart by imaging planes was achieved with high time resolution (13 ms), with typically 12 time frames in the cardiac cycle, using a short echo time (5 ms) multislice gradient‐echo imaging sequence. Imaging was synchronized to the R wave of the electrocardiogram. The image slices could be reconstructed into complete geometrically and temporally coherent three‐dimensional data sets. Left ventricular (LV) volumes were thus reconstructed throughout the cardiac cycle by combining transverse cardiac image sections. This volume analysis revealed structural and functional differences between the normal WKY and SHR in both age groups of 8 and 12 weeks. Measurements from the cardiac images were additionally validated against histological measurements. The SHR showed a raised LV end‐systolic volume and a correspondingly poorer ejection fraction as well as LV hypertrophy when compared with the controls. Left ventricular function in the SHR appeared stable between the two age groups. We developed a simple geometrical model of the left ventricle based on a single longitudinal image section and successfully used this to describe some functional parameters of the left ventricle in the WKY and SHR. This geometrical model has the potential to greatly reduce the imaging time needed to study the beating heart in future serial investigations of cardiac physiology in rats. Our experimental and analytical methods together form a powerful set of quantitative techniques which combine both imaging and functional analysis and will be applicable for future studies of chronic physiological changes in animal disease models.

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“…Hence, the majority of physiological MRI applications use semiquantitative analysis (i.e., parameter values in the region of interest in relation to a reference tissue). An exemption remains cardiological applications, in which absolute values of functional parameters like stroke volume and ejection fraction can be derived by MRI, which is essentially based on morphometric measurements [22].…”

Section: Benefits and Limitations Of In Vivo Mr Techniquesmentioning

confidence: 99%

In Vivo magnetic resonance techniques and drug discovery

Beckmann¹

2006

Braz. J. Phys.

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The long and resource intensive process of drug discovery and development is confronted with the basic challenge of providing effective and safe therapies at reasonably low costs. The better the mechanism of a disease is known, the higher the probability to find an appropriate therapy. Also, the better and earlier a disease can be diagnosed and characterized, the higher the chance to be able to interfere in this process with a chemical entity. This reasoning sets the framework for the use of imaging in drug discovery. We discuss the relevance of magnetic resonance imaging and spectroscopy to derive anatomical, functional, metabolic and target-related information in the context of pharmacological research in vivo.

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Determination of rat heart morphology and function in vivo in two models of cardiac hypertrophy by means of magnetic resonance imaging

Cited by 46 publications

References 13 publications

A model of chronic heart failure in spontaneous hypertensive rats (SHR)

A model of chronic heart failure in spontaneous hypertensive rats (SHR)

Magnetic resonance imaging analysis of left ventricular function in normal and spontaneously hypertensive rats

In Vivo magnetic resonance techniques and drug discovery

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