Determination of ractopamine residue in animal derived foods using electromembrane extraction followed by liquid chromatography tandem mass spectrometry

Gao, Haidong; Chen, Mantang; Gao, Huoliang; Gao, Shucai; Liu, Xiao; Du, Rui; Wang, Fayun; Wang, Yong; Wan, Libin

doi:10.1016/j.chroma.2022.463179

Cited by 8 publications

(9 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Additionally, the matrix effect was evaluated for fungi-derived food using slope ratios of matrix-matched standard curves to the solvent standard calibration curve [33]. In a previous animal-derived food test, the matrix effects for ractopamine in bovine muscle, porcine muscle, lamb muscle, and porcine liver were 0.17, 0.23, 0.22, and 0.27, respectively [9]. Similarly, the results of the present study showed that the matrix effects for clenbuterol, ractopamine, and salbutamol were 0.46, 0.32, and 0.42, respectively.…”

Section: Discussionmentioning

confidence: 99%

“…Beta-agonists have been approved as growth promoters in animal feed [8]. An acceptable ractopamine daily intake of 0-1 μg/kg bodyweight for food animals was established by the Food and Agriculture Organization, and maximum ractopamine residue limits of 10 μg/kg in the muscle and fat, 90 μg/kg in the kidney, and 40 μg/kg in the liver have been recommended [9,10]. The maximum residue limits of clenbuterol confined by the Codex Alimentarius Commission and Food and Drug Administration (FDA) are 0.6 pg/mg in the liver and kidney and 0.2 pg/mg in the muscle and fat [11].…”

Section: Introductionmentioning

confidence: 99%

“…Several analytical methods have been developed and applied for the detection of β-agonists in animal feedstuffs, tissues, and urine, including high performance liquid chromatography (HPLC) [20], liquid chromatography tandem mass spectrometry (LC-MS/ MS) [21], fluorescence immunoassay [22], enzymelinked immunosorbent assay [23], and rapid electrochemical detection based on an electrochemical (g-C 3 N 4 /Cu@CoO/NC/GCE) sensor [24]. LC-MS/MS is considered the national standard technique and is the most extensively used approach for the determination of β-agonist residues because of its high sensitivity and accuracy [9,25].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Transfer of β‐agonists from animal feed into Tricholoma gambosum through manure

Han,

Zhan,

Zhang

et al. 2023

Animal Research and One Health

View full text Add to dashboard Cite

Fungi are dependent on animal manure as a cultivation medium and may be vulnerable to feed‐derived β‐agonist contamination. To test whether β‐agonists incorporated in animal feed can transport into fungi through manure, a greenhouse study was conducted with Tricholoma gambosum grown in a culture medium amended with medicated cattle manure. Cattle were orally administrated with a single (ractopamine, 670.0 μg/kg BW/day) or a mixture of β‐agonists (clenbuterol, ractopamine, and salbutamol at the doses of 5.3, 223.3, and 50.0 μg/kg BW/day, respectively) for 28 days. Three batches of T. gambosum were harvested. A liquid chromatography tandem mass spectrometry‐based method was developed to quantify the number of β‐agonists taken up by T. gambosum from animal manure. The analytical recoveries for β‐agonists were between 66.61% and 91.78% with relative standard deviations between 1.70% and 12.18%, and the limit of quantification (LOQ) was 0.3 ng/g. The ractopamine residues in T. gambosum from batch 1 were 1.3 ng/g and were below the LOQ in batches 2 and 3 in the single treatment group. In the mixed treatment group, ractopamine concentrations were 0.42 and 0.50 ng/g in batches 1 and 2, respectively, and the salbutamol concentration was 1.94 ng/g in batch 1, while clenbuterol was undetectable in all three batches. These results indicated that the β‐agonists transferred to T. gambosum in trace amounts and presented a limited risk to consumers.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Transfer of β‐agonists from animal feed into Tricholoma gambosum through manure

Han,

Zhan,

Zhang

et al. 2023

Animal Research and One Health

View full text Add to dashboard Cite

show abstract

“…Currently, analytical methods for detecting Rac in pork are limited to techniques requiring highly sophisticated sample preparation or costly instrumentation, such as enzyme-linked immunosorbent assay (ELISA), high-performance liquid chromatography (HPLC), gas chromatography–mass spectrometry (GC–MS), and liquid chromatography–tandem mass spectrometry (LC–MS/MS). − ELISA offers a rapid and portable method for detecting Rac; however, its major drawback is its requirements of complicated time-consuming sample pretreatment and preparation because of the complex matrix of animal tissues . Among instrumental techniques, HPLC enables multicomponent and highly sensitive analysis; however, the HPLC instrument is costly and nonportable, which prevents its widespread application . Moreover, well-trained personnel are required to operate this instrument.…”

Section: Introductionmentioning

confidence: 99%

“…14 Among instrumental techniques, HPLC enables multicomponent and highly sensitive analysis; however, the HPLC instrument is costly and nonportable, which prevents its widespread application. 18 Moreover, welltrained personnel are required to operate this instrument. Implementing GC−MS in Rac detection is difficult because of the high boiling point of Rac.…”

Section: Introductionmentioning

confidence: 99%