Determination of Quinoxalines and Their Two Main Metabolites in Environmental Water Samples by Liquid Chromatography–Tandem Mass Spectrometry

Yang, Haicui; He, Lili; Liu, Yahong; Bian, Kui; Hu, Fengyang; Fang, Binghu

doi:10.1080/00032719.2013.867497

Cited by 2 publications

(1 citation statement)

References 21 publications

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“…To confirm the reliability of the developed ic-ELISA method, four spiked chicken samples were selected for comparison using ic-ELISA analysis and LC–MS/MS. The detailed procedures for LC–MS/MS analysis and sample preparation were performed according to the previous description [ 20 ]. Briefly, 10 mL of methanol-water ( v / v , 5:95) and 20 mg of N-propyl ethylenediamine were added to the homogenized samples.…”

Section: Methodsmentioning

confidence: 99%

A Novel Metabolite as a Hapten to Prepare Monoclonal Antibodies for Rapid Screening of Quinoxaline Drug Residues

Song

Luo

et al. 2022

Foods

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Quinoxalines (Qx) are chemically synthesized antibacterial drugs with strong antibacterial and growth-promoting effects. Qx is heavily abused by farmers, resulting in large residues in animal-derived foods, which pose a serious threat to human health. Desoxyquinoxalines (DQx), which have the highest residue levels, have been identified as the major toxicant and have become a new generation of residue markers. In this study, we prepared monoclonal antibodies (mAb) based on a new generation metabolite (desoxymequindox, DMEQ) and establish an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) for the rapid determination of Qx residues in food. The mAb exhibited high sensitivity with half maximal inhibitory concentration (IC50) and a linear range of 2.84 µg/L and 0.8–12.8 µg/L, respectively. Additionally, the cross-reactivity (CR) of the mAb showed that it recognized multiple DQx to varying levels. The limits of detection (LOD), limits of quantification (LOQ), and recoveries for the ic-ELISA assay of pork, swine liver, swine kidney, chicken, and chicken liver were 0.48–0.58 µg/kg, 0.61–0.90 µg/kg, and 73.7–107.8%, respectively, and the coefficients of variation (CV) were less than 11%. The results of the ic-ELISA showed a good correlation with LC–MS/MS in animal-derived foods. This suggests that this analytical method can be used for the rapid screening of QX residues.

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Section: Methodsmentioning

confidence: 99%