Determination of quinolones in chicken tissues by liquid chromatography with ultraviolet absorbance detection

Bailac, S; Ballesteros, O.; Jiménez-Lozano, E; Barrón, D.; Sanz‐Nebot, Victoria; Navalón, Alberto; Vı́lchez, J.L.; Barbosa, J.

doi:10.1016/j.chroma.2003.11.110

Cited by 70 publications

(57 citation statements)

References 22 publications

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“…In several animal species, including pigs, enrofloxacin (ENRO) is de-ethylated to its primary metabolite, ciprofloxacin (CIPRO), 2 and both ENRO and CIPRO are found in the bile and urine of animals receiving ENRO. 3 Several analytical techniques have been used for the determination of ENRO and CIPRO (Table 1), including liquid chromatography, [4][5][6][7] capillary electrophoresis with diode array detection, 8 and molecularly imprinted matrix solid-phase dispersion. 9 Electrochemical methods, such as polarography and voltammetry, have high sensitivity and are widely used in many areas of analytical chemistry.…”

mentioning

confidence: 99%

Simultaneous Voltammetric Determination of Enrofloxacin and Ciprofloxacin in Urine and Plasma Using Multiwall Carbon Nanotubes Modified Glassy Carbon Electrode by Least-Squares Support Vector Machines

et al. 2010

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803Fluoroquinolones are a class of synthetic antibacterial agents, which are known for their broad spectrum of antibacterial activity. These compounds are derived from nalidixic acid and naphthyridine derivatives, which have been introduced for clinical applications in the livestock and farming industries.1 In this work, two important fluoroquinolones, namely enrofloxacin (1-cyclopropyl-7-(4-ethyl-1-piperazinyl)-6-fluoro-1,4-dihydro-4-oxo-3-quinolonecarboxylic acid) and ciprofloxacin (1-cyclopropyl-7-(1-piperazinyl)-6-fluoro-1,4-dihydro-4-oxo-3-quinolinecarboxylic acid), were investigated and quantitatively analyzed by voltammetric methods. In several animal species, including pigs, enrofloxacin (ENRO) is de-ethylated to its primary metabolite, ciprofloxacin (CIPRO), 2 and both ENRO and CIPRO are found in the bile and urine of animals receiving ENRO. Electrochemical methods, such as polarography and voltammetry, have high sensitivity and are widely used in many areas of analytical chemistry.Recently, cathodic adsorptive stripping voltammetry on a static mercury drop electrode and principal-component regression have been applied for the determination of ENRO in the presence of CIPRO 10 at trace levels. However, the linear dynamic range of the method is too narrow, and the toxicity of mercury (as an electrode) has caused its application in routine laboratories to decline. At more negative potentials, many biological compounds are adsorbed on the surface of the Hg-electrode, diminishing the selectivity of the method. 10 On the other hand, simultaneous voltammetric determinations of ENRO and CIPRO have not been reported, since ENRO affects the oxidation potential of CIPRO and vice versa. Therefore, the direct determination of CIPRO in the presence of ENRO is not possible by univariate calibration methods.In this work, linear sweep voltammetry (LSV) used for the first time with a multiwall carbon nanotubes/glassy carbon electrode (MWCNT/GCE) provides a sensitive method for the determination of ENRO or CIPRO in the presence of the other without any pretreatment or separation. When MWCNT/GCE is used as a working electrode in the voltammetric method, ENRO affects the oxidation potential of CIPRO, whereas CIPRO does not affect that of ENRO. Because it was possible to measure ENRO without any interfering effect of CIPRO, a least-squares support vector machine (LS-SVM) model was constructed in this study to relate a CIPRO voltammogram to its concentration. The model predicts CIPRO concentration in both spiked human plasma and pharmaceutical samples. The proposed methodology is fast and simple, while it does not generate hazardous chemical wastes. Theory of Least Square Support Vector RegressionSupport vector machines (SVMs) based on statistical learning theory were introduced by Cortes and Vapnik.11 LS-SVMs are an alternate formulation of SVM described by Suykens et al. 12 Here, we only briefly describe the theory of LS-SVM. Linear least-squares models fit a linear relation between the spectrum xi and the reference va...

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confidence: 99%

Simultaneous Voltammetric Determination of Enrofloxacin and Ciprofloxacin in Urine and Plasma Using Multiwall Carbon Nanotubes Modified Glassy Carbon Electrode by Least-Squares Support Vector Machines

et al. 2010

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“…Most of them involve liquid chromatography with ultraviolet (LC-UV; Gigosos et al, 2000;Pecorelli et al, 2003;Bailac et al, 2004;Christodoulou and Samanidou, 2007), fluorescence (LC-FD;Eng et al, 1998;Hernández-Arteseros et al, 2000;Chu et al, 2002;EspinosaMansilla et al, 2005;Hassouan et al, 2007) or mass spectrometric detection (Van Vyncht et al, 2002;Toussaint et al, 2002Toussaint et al, , 2005aSchneider et al, 2005;Van Hoof et al, 2005;Hermo et al, 2006;Bailac et al, 2006;Rubies et al, 2007).…”

Section: Introductionmentioning

confidence: 98%

Simultaneous determination of quinolone antibacterials in bovine milk by liquid chromatography–mass spectrometry

Zafra-Gómez

Garballo

Ballesteros

et al. 2008

Biomedical Chromatography

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A new liquid chromatography-mass spectrometry (LC-MS) method has been developed and validated for the simultaneous determination of eight quinolone antibacterials for veterinary use in processed bovine milk samples. The quinolones studied included marbofloxacin, ciprofloxacin, danofloxacin, enrofloxacin, sarafloxacin, difloxacin, oxolinic acid and flumequine. Also, a new sample-treatment procedure was used for extraction and preconcentration of these compounds. It involved defatting by centrifugation, protein precipitation by adding a mixture of glacial acetic acid-acetonitrile and removing acetonitrile with dichloromethane; finally, the acidified aqueous layer was evaporated to dryness in a speed vac system, resuspended in the mobile phase and filtered prior to LC injection. The mobile phase was composed of a formic acid aqueous solution 0.1% (v/v) and acetonitrile, with an initial composition of water-acetonitrile 95: 5 (v/v) and using linear gradient elution. Norfloxacin was used as internal standard. The limits of quantification found (2-7 ng g(-1)) were in all cases lower than the maximum residue limits tolerated by the European Union for these compounds in milk.

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“…For quinolones, liquid chromatography (LC) is generally used for separation and fluorescence detection is extensively applied to LC because of its good selectivity and sensitivity. 5,6 But thin-layer chromatography (TLC)-fluorescence, 7-9 capillary electrophoresis-fluorescence, 10 highperformance liquid chromatography (HPLC)-UV or photodiode-array 4 have been also used. For NSAID, the methods of double beam UV spectrophotometry, 11 capillary electrophoresis, 12 gas chromatography (GC), 13 electrospray ionization mass spectrometry (ESI-MS) 14 and liquid chromatography with UV detection 15,16 have been used widely.…”

Section: Introductionmentioning

confidence: 99%

“…Many countries and organizations have established maximum residue limits (MRL) for antibiotics residues in animal tissues. 4 Monitoring of these residues is necessary to develop sensitive multi-residue screening methods to ensure that human food is entirely free from potentially harmful residues.…”

Section: Introductionmentioning

confidence: 99%

Determination of Quinolones and Nonsteroidal anti‐Inflammatory Agents in Animal Tissues and Bovine Milk by Microwave‐assisted Extraction High Performance Liquid Chromatography

Xi-liu

Liu²,

Cheng

et al. 2008

Chin. J. Chem.

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A rapid, specific microwave-assisted extraction high performance liquid chromatography is described for assaying three quinolones (fleroxacin, lomefloxacin and sparfloxacin) and two nonsteroidal anti-inflammatory agents (ketoprofen and ibuprofen) in samples of sheep liver, bovine muscle and milk. The optimal microwave-assisted extraction conditions such as extraction temperature (40 ), extraction time (6 min), solvent volume (10 mL) and ℃ solvent (acetonitrile) were determined by an orthogonal experiment. Recoveries were 60.0%-107% in the concentration range 0.25-0.75 µg•g -1 with good precision (＜11%) from three varieties of spiked animal samples.

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Determination of quinolones in chicken tissues by liquid chromatography with ultraviolet absorbance detection

Cited by 70 publications

References 22 publications

Simultaneous Voltammetric Determination of Enrofloxacin and Ciprofloxacin in Urine and Plasma Using Multiwall Carbon Nanotubes Modified Glassy Carbon Electrode by Least-Squares Support Vector Machines

Simultaneous Voltammetric Determination of Enrofloxacin and Ciprofloxacin in Urine and Plasma Using Multiwall Carbon Nanotubes Modified Glassy Carbon Electrode by Least-Squares Support Vector Machines

Simultaneous determination of quinolone antibacterials in bovine milk by liquid chromatography–mass spectrometry

Determination of Quinolones and Nonsteroidal anti‐Inflammatory Agents in Animal Tissues and Bovine Milk by Microwave‐assisted Extraction High Performance Liquid Chromatography

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