Determination of quinapril and quinaprilat in human plasma by ultraperformance liquid chromatography–electrospray ionization mass spectrometry

Abstract

A novel, specific and sensitive ultraperformance liquid chromatography tandem mass spectrometry (UPLC-MS/ MS) method was developed for the simultaneous determination of quinapril and its active metabolite quinaprilat in human plasma. The method involves a simple, one-step extraction procedure coupled with an Acquity UPLC TM BEH C 18 column (100 × 2.1 mm, i.d., 1.7 mm) with isocratic elution at a flow-rate of 0.2 mL/min and lisinopril as the internal standard. Detection was performed on a triple-quadrupole tandem mass spectrometer in multiple reaction monitoring mode via electrospray ionization. Using 250 mL plasma, the methods were validated over the concentration range 5.010-500.374 ng/mL for quinapril and 10.012-1000 ng/mL for quinaprilat, with a lower limit of quantification of 5.010 ng/mL for quinapril and 10.012 ng/mL for quinaprilat. The intra-and inter-day precision and accuracy were within 10.0%. The recovery was 85.8, 62.6 and 61.3% for quinapril, quinaprilat and lisinopril, respectively. Total run time was 3.0 min only.

Retrospective LC‐QTOF‐MS analysis searching for pharmaceutical metabolites in urban wastewater

“…6. The ions at m/z 233, 139 and 121 observed in the HE TOF spectra matched with the product ions reported in a previous LC‐MS/MS method using triple quadrupole 30, strongly supporting the identity suggested for this metabolite. This metabolite was found in 14 of the 24 samples analysed (around 58%).…”

Effects of quinapril on angiotensin converting enzyme and plasma renin activity as well as pharmacokinetic parameters of quinapril and its active metabolite, quinaprilat, after intravenous and oral administration to mature horses

“…The concentrations of quinapril and quinaprilat were determined by ultra‐high pressure liquid chromatography with tandem mass spectrometry using a method adapted from a previously described technique and validated in equine plasma. Calibration curves were prepared daily by fortifying blank plasma with stock solutions of quinapril c and quinaprilat c dissolved in methanol at a concentration of 1 mg/ml each, then further diluted in a solution of methanol and double distilled water (50:50 v/v).…”

Forced Degradation Study of Quinapril by UPLC-DAD and UPLC/ MS/MS: Identification of By-products and Development of Degradation Kinetics

“…Many analytical methods have been reported in the literature for the analysis of quinapril such as gas chromatography with negative-ion chemical ionization mass spectrometry (MS) or electron-capture detection [24], capillary electrophoresis [25], high performance liquid chromatography with fluorescence [24], UV [2] or radiochemical detection [26] as well as electrospray ionization (HPLC/ESI) [2], and MALDI-TOF MS [27]. The considered techniques include also UV spectroscopy [28], square wave voltammetry [29], ultraperformance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) [28], ultra-thin-layer chromatography mass spectrometry and thin-layer chromatography mass spectrometry [30]. However, to our knowledge, on the one hand no study demonstrates the ability of ultra-performance liquid chromatography (UPLC) to resolve the separation of quinapril and its degradation products, and on the other hand the identification of the by-products has not been previously investigated.…”