Determination of purine contents in different parts of pork and beef by high performance liquid chromatography

Rong, Shengzhong; Zou, Lina; Zhang, Yannan; Zhang, Guangteng; Li, Xiaoxia; Li, Miaojing; Yang, Fenghua; Li, Chunmei; He, Yingjuan; Guan, Hongjun; Guo, Yupeng; Wang, Dong; Cui, Xinyu; Ye, Hongting; Liu, Fenghai; Pan, Hongzhi; Yang, Yuexin

doi:10.1016/j.foodchem.2014.08.059

Cited by 62 publications

(33 citation statements)

References 25 publications

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“…Many kinds of C 18 column have been used to separate the four purines . Both Agilent XDB‐C 18 and Waters Atlantis dC 18 columns were chosen in this study, because the Agilent XDB‐C 18 was a preferred column to establish a new method and the Waters Atlantis dC 18 was excellent in separating polar compounds.…”

Section: Resultsmentioning

confidence: 99%

“…Adenine, guanine, hypoxanthine and xanthine exhibited strong absorption at 254 nm . Therefore, 254 nm was fixed as the detection wavelength.…”

Section: Resultsmentioning

confidence: 99%

“…Furthermore, available technologies have limited determination methods of purine, thereby hampering purine data updates. In recent years, the determination of purine in foods has become of wide concern once again, with various determination methods focused on beer, soybean, and livestock and poultry meat . Seafood is widely consumed for its tasty flavor and high content of protein and unsaturated fatty acids.…”

Section: Introductionmentioning

confidence: 99%

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Determination of four different purines and their content change in seafood by high-performance liquid chromatography

Sui

et al. 2016

J. Sci. Food Agric.

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Section: Resultsmentioning

confidence: 99%

“…Adenine, guanine, hypoxanthine and xanthine exhibited strong absorption at 254 nm . Therefore, 254 nm was fixed as the detection wavelength.…”

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Determination of four different purines and their content change in seafood by high-performance liquid chromatography

Sui

et al. 2016

J. Sci. Food Agric.

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“…The detection limit values obtained for A and G in this work are about 10 times lower than previously reported HPLC based UV−vis detection approaches. 21,23,24 …”

Section: Langmuirmentioning

confidence: 99%

In Situ Derivatization of an Intrinsic Iron Impurity as a Surface-Confined Iron(II)tris(2,2′-bipyridine) Complex on MWCNT and Its Application to Selective Electrochemical Sensing of DNA’s Purine Bases

Mayuri

Kumar

2015

Langmuir

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The derivatization of an intrinsic iron impurity in a carbon nanotube (CNT-*Fe, *Fe-intrinsic, and redoxactive iron impurity) as a functional molecular system has been challenging to realize. There are certain limitations on the derivatization of such iron impurities such as low concentration and limited accessibility. Herein, we report an in situ electroassisted derivatization of an intrinsic and redox-active iron impurity in a multiwalled carbon nanotube (MWCNT-*Fe, *Fe, 2.1 wt %) as MWCNT-*Fe(bpy) 3 2+ , where Fe(bpy) 3 2+ = iron(II)tris(2,2′-bipyridine) complex and bpy = 2,2′-bipyridine. The hybrid complex was prepared by the electrochemical treatment of a 2,2′-bipyridine ligand adsorbed {MWCNT-*Fe + Nafion} modified glassy carbon electrode in pH 7 phosphate buffer solution. This new MWCNT-*Fe(bpy) 3 2+ hybrid electrode showed well-defined, stable redox at E 1/2 = 830 mV with a peak-to-peak separation (ΔE p ) of 72 mV in a neutral pH solution. This is quite different from an ex situ NafionFe(bpy) 3 2+ complex system that showed an unstable response at neutral pH. This in situ approach can allow the redox-active iron impurity in the CNTs to be quantified using the current signal of the Fe(bpy) 3 2+ hybrid system. This MWCNT-*Fe(bpy) 3 2+ hybrid modified electrode was further used as an electrochemical detector for selective and separation-less flow injection analysis of DNA's purine bases, adenine and guanine, without interference from pyrimidine bases, cytosine, and thymine at different oxidative detection potentials of 1 V (for adenine and guanine) and 0.7 V vs Ag/AgCl (for guanine) using the pH 7 phosphate buffer solution as a carrier system.

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“…Nowadays, different analytical methods such as liquid chromatography, electrophoresis coupled with various detection techniques have been developed for the detection of purine bases in the nucleic acid [15][16][17]. Although these methods are with good sensitivity and accuracy, they require complicated apparatus and time-consuming experimental procedures.…”

Section: G] = [C] and [A] = [T]mentioning

confidence: 99%

Electrospun nickel loaded porous carbon nanofibers for simultaneous determination of adenine and guanine

Luo

Ding

et al. 2015

Electrochimica Acta

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Determination of purine contents in different parts of pork and beef by high performance liquid chromatography

Cited by 62 publications

References 25 publications

Determination of four different purines and their content change in seafood by high-performance liquid chromatography

Determination of four different purines and their content change in seafood by high-performance liquid chromatography

In Situ Derivatization of an Intrinsic Iron Impurity as a Surface-Confined Iron(II)tris(2,2′-bipyridine) Complex on MWCNT and Its Application to Selective Electrochemical Sensing of DNA’s Purine Bases

Electrospun nickel loaded porous carbon nanofibers for simultaneous determination of adenine and guanine

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