2005
DOI: 10.1586/14789450.2.5.745
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Determination of protein-derived epitopes by mass spectrometry

Abstract: Mass spectrometry has evolved as a technique suitable for the characterization of peptides and proteins beyond their linear sequence. The advantages of mass spectrometric sample analysis are high sensitivity, high mass accuracy, rapid analysis time and low sample consumption. In epitope mapping, the molecular structure of an antigen (the epitope or antigenic determinant) that interacts with the paratope (recognition surface) of the antibody is identified. To obtain information on linear, conformational and/or … Show more

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Cited by 69 publications
(60 citation statements)
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“…The rate at which backbone amide hydrogens exchange in solution is directly dependent on the dynamics and structure of the protein. Therefore, regions embedded within the structure or occluded by the presence of a ligand will exchange more slowly than regions fully exposed to the solvent (24).…”
Section: Recombinant C-terminal Domain Of Fhbp Binds Mab 12c1 With Lowermentioning
confidence: 99%
“…The rate at which backbone amide hydrogens exchange in solution is directly dependent on the dynamics and structure of the protein. Therefore, regions embedded within the structure or occluded by the presence of a ligand will exchange more slowly than regions fully exposed to the solvent (24).…”
Section: Recombinant C-terminal Domain Of Fhbp Binds Mab 12c1 With Lowermentioning
confidence: 99%
“…4E10 binds to a highly conserved linear epitope on MPER 671 NWF(D/N)IT 676 (161). Crystallographic structures of the epitope have been characterized and form a large helical shape with the important residues on the same side of the helix (44). The antigen reacts only with residues of the base and center of the loop of CDRH3.…”
Section: Gp41 Immunogenicitymentioning
confidence: 99%
“…The 671 NWFDITNWLWYI K 683 sequence is optimal for 4E10 recognition. In resistant strains, the pattern NWF(N/D)IT indicates that the 4E10 epitope is complex and would even be discontinuous (44). Mass spectrometry studies reveal that 4E10 binds to the N terminus of gp120 and gp41 on the native conformation (130).…”
Section: Gp41 Immunogenicitymentioning
confidence: 99%
“…When the antigen-antibody complex forms, the interface between the binding partners can occlude solvent accessibility, reducing the exchange rate. 16 Epitope mapping by HDX-MS was carried out in 2 parallel steps: deuterium incorporation was performed on NadA alone (reference experiment) and on the antigen-antibody complex, as previously described.…”
Section: Epitope Mapping Using Hdx-msmentioning
confidence: 99%