Determination of protein concentration by total organic carbon analysis

Rouwenhorst, R.J.; Jzn, J. Frank; Scheffers, W. A.; Dijken, Johannes P. van

doi:10.1016/0165-022x(91)90024-q

Cited by 62 publications

(34 citation statements)

References 12 publications

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“…5a) (Savidge, 2000;Lamlom and Savidge, 2003;Bert and Danjon, 2006;Martin and Thomas, 2011). Despite the high lignin in roots, the C content in roots was lower than that in leaves, probably because of the high proportions of protein and other C-rich compounds in leaves (Rouwenhorst et al, 1991;Niinemets et al, 2002) and high content of starch in roots (Bert and Danjon, 2006). The lowest C content in reproductive organs was consistent with its high quantities of NSC and low content of lignin (Barros et al, 1996).…”

Section: Discussionmentioning

confidence: 68%

Variations and determinants of carbon content in plants: a global synthesis

Ma¹,

He²,

Tian³

et al. 2018

Biogeosciences

221

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Abstract. Plant carbon (C) content is one of the most important plant traits and is critical to the assessment of global C cycle and ecological stoichiometry; however, the global variations in plant C content remain poorly understood. In this study, we conducted a global analysis of the plant C content by synthesizing data from 4318 species to document specific values and their variation of the C content across plant organs and life forms. Plant organ C contents ranged from 45.0 % in reproductive organs to 47.9 % in stems at global scales, which were significantly lower than the widely employed canonical value of 50 %. Plant C content in leaves (global mean of 46.9 %) was higher than that in roots (45.6 %). Across life forms, woody plants exhibited higher C content than herbaceous plants. Conifers, relative to broad-leaved woody species, had higher C content in roots, leaves, and stems. Plant C content tended to show a decrease with increasing latitude. The life form explained more variation of the C content than climate. Our findings suggest that specific C content values of different organs and life forms developed in our study should be incorporated into the estimations of regional and global vegetation biomass C stocks.

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Section: Discussionmentioning

confidence: 68%

Variations and determinants of carbon content in plants: a global synthesis

Ma¹,

He²,

Tian³

et al. 2018

Biogeosciences

221

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“…However, in addition to protein, cell-free extracts are likely to contain other organic compounds, such as lipids, nucleic acids and carbohydrates (Rouwenhorst et al, 1991). Therefore, the protein concentration in the initial cell-free extracts may be somewhat overestimated.…”

Section: Purification and Physical Propertiesmentioning

confidence: 99%

“…After addition of 1 mM trithionate, 0.5-ml samples were taken after the desired time intervals and immediately mixed with 0.1 ml purified fractions can be calculated by assuming that proteins have an average carbon content of 0.53 gig (Rouwenhorst et al, 1991).…”

Section: Discontinuous Assay For Activity Measurementsmentioning

confidence: 99%

“…Active Due to the high ammonium sulphate concentrations in the enzyme extracts, the protein determination methods of Lowry (1 951) and Bradford (1976) could not be used. Therefore protein concentration was measured by determination of the total-organic-carbon content of extracts as described by Rouwenhorst et al (1991). Protein concentration in (partly) fractions were concentrated by ultrafiltratlon.…”

Section: Gelfiltrationmentioning

confidence: 99%

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Purification and paritial characterization of a thermostable trithionate hydrolase from the acidophilic sulphur oxidizer Thiobacillus acidophilus

Meulenberg¹,

Pronk²,

Frank³

et al. 1992

European Journal of Biochemistry

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Ccll-free extracts of Thiobacillus acidophilus catalysed the quantitative conversion of trithionate (S,Oz -) to thiosulphate and sulphate. A continuous assay for quantification of experimental results was based on the difference in absorbance between trithionate and thiosulphate at 220 nm.Trithionate hydrolase was purified to near homogeneity from cell-free extracts of T . acidophilus. The molecular masses of the native enzyme and the subunit were 99 kDa (gel filtration) and 34 kDa (SDS/PAGE). The purified enzyme has a pH optimum of 3.5-4.5 and a temperature optimum of 70 "C. Enzyme activity was stimulated by sulphate. The stimulation of the enzyme activity by sulphate was half maxima! at a concentration of 0.23 M. The K, for trithonate is 70 pM at 30°C and 270 pM at 70°C. Enzyme activity was lost after 36 days at O"C, 27 days at 70°C; but after 97 days at 30"C, 40% of the initial activity was still present. The enzyme activity was inhibited by mercury chloride, .hi-ethylmaleimidc, thiosulphate and tetrathionate. Tetrathionate S402-was not hydrolysed by trithionate hydrolase.

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“…This comparison is somewhat uncertain, as we had to convert Polz's data, expressed per weight unit of protein, into values given per weight unit of total carbon, the carbon content of proteins being estimated at 45% (Rouwenhorst et al, 1991). Yet, the consistently higher rates found here might be due to the incubation of live shrimps under in-situ pressure (230 bars in this study), as opposed to the use, in previous experiments, of pieces of dead shrimp under atmospheric pressure.…”

Section: Carbon Fixation By Bacterial Chemosynthesismentioning

confidence: 99%

Inorganic carbon fixation by chemosynthetic ectosymbionts and nutritional transfers to the hydrothermal vent host-shrimp Rimicaris exoculata

et al. 2012

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The shrimp Rimicaris exoculata dominates several hydrothermal vent ecosystems of the MidAtlantic Ridge and is thought to be a primary consumer harbouring a chemoautotrophic bacterial community in its gill chamber. The aim of the present study was to test current hypotheses concerning the epibiont's chemoautotrophy, and the mutualistic character of this association. Invivo experiments were carried out in a pressurised aquarium with isotope-labelled inorganic carbon (NaH 13 CO 3 and NaH 14 CO 3 ) in the presence of two different electron donors (Na 2 S 2 O 3 and Fe 2 þ ) and with radiolabelled organic compounds ( 14 C-acetate and 3 H-lysine) chosen as potential bacterial substrates and/or metabolic by-products in experiments mimicking transfer of small biomolecules from epibionts to host. The bacterial epibionts were found to assimilate inorganic carbon by chemoautotrophy, but many of them (thick filaments of epsilonproteobacteria) appeared versatile and able to switch between electron donors, including organic compounds (heterotrophic acetate and lysine uptake). At least some of them (thin filamentous gammaproteobacteria) also seem capable of internal energy storage that could supply chemosynthetic metabolism for hours under conditions of electron donor deprivation. As direct nutritional transfer from bacteria to host was detected, the association appears as true mutualism. Import of soluble bacterial products occurs by permeation across the gill chamber integument, rather than via the digestive tract. This first demonstration of such capabilities in a decapod crustacean supports the previously discarded hypothesis of transtegumental absorption of dissolved organic matter or carbon as a common nutritional pathway.

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Determination of protein concentration by total organic carbon analysis

Cited by 62 publications

References 12 publications

Variations and determinants of carbon content in plants: a global synthesis

Variations and determinants of carbon content in plants: a global synthesis

Purification and paritial characterization of a thermostable trithionate hydrolase from the acidophilic sulphur oxidizer Thiobacillus acidophilus

Inorganic carbon fixation by chemosynthetic ectosymbionts and nutritional transfers to the hydrothermal vent host-shrimp Rimicaris exoculata

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