Determination of prostaglandins D2, E2, F2α and 6-ketoprostaglandin F1α as well as thromboxane B2 in rat brain by gas chromatography-mass spectrometry

Weber, Claudia; Belsner, Klaus; Lohkamp, Reinhard; Höller, Michael

doi:10.1016/s0378-4347(00)81961-1

Cited by 8 publications

(2 citation statements)

References 12 publications

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“…The capillary column was directly introduced into the ion source of a mass spectrometer (Finnigan MAT 8230). The com pounds were determined by negative ion chemical ioniza tion mass spectrometry as pentafluorobenzyl ions using ammonia as reagent gas (Weber et al, 1988). Registration and quantification were carried out by multiple ion detec tion.…”

Section: Gas Chromatography/mass Spectrometrymentioning

confidence: 99%

Eicosanoids in Rat Brain during Ischemia and Reperfusion—Correlation to DC Depolarization

Tegtmeier

Weber

Heister

et al. 1990

J Cereb Blood Flow Metab

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Summary:The effects of complete ischemia on cerebral arachidonic acid (AA) metabolism were investigated in the isolated perfused rat brain. During 12.5 min of isch emia, AA, 5-hydroxy-6,8, 11, 14-eicosatetraenoic acid, and 15-hydroxy-5 ,8,11, 13-eicosatetraenoic acid increased 129-, 4-, and 10-fold, respectively, while subsequent reperfusion for 30 min resulted in normalized levels inde pendently of the duration of preceding ischemia. Prosta glandin (PG) F2u, PGE2, PGD2, 6-keto-PGF,u, and thromboxane (Tx) B2 remained at preischemic levels dur ing 12.5 min of complete ischemia. However, at the end of subsequent reperfusion for 30 min, the levels of the

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Section: Gas Chromatography/mass Spectrometrymentioning

confidence: 99%

Eicosanoids in Rat Brain during Ischemia and Reperfusion—Correlation to DC Depolarization

Tegtmeier

Weber

Heister

et al. 1990

J Cereb Blood Flow Metab

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show abstract

“…The plates were developed with the organic phase of ethyl acetate: acetic acid: hexane: water (54:12:25:100). Prostanoids were eluted from the silica gel and derivatized to their pentafluorobenzyl esters-trimethylsilyl ethers (24). GC was performed on a Hewlett Packard 5890 chromatograph equipped with a cooled injection system, and an Ultra 2 column.…”

Section: Determination Of Urinary Prostanoidsmentioning

confidence: 99%

Ridogrel Inhibits Systemic and Renal Formation of Thromboxane A2 and Antagonizes Platelet Thromboxane A2/Prostaglandin Endoperoxide Receptors upon Chronic Administration to Man

et al. 1992

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SummaryThe effects of ridogrel, a dual thromboxane A2 (TXA2) synthase inhibitor and TXA2/prostaglandin (PG) endoperoxide receptor antagonist, on systemic and renal production of prostaglandins and on platelet TXA2/PG endoperoxide receptors was evaluated upon chronic administration (300 mg b. i. d. orally, for 8 and 29 days) to man. Such a medication with ridogrel inhibits the systemic as well as the renal production of TXA2 as measured by the urinary excretion of 2,3-dinor-TXB2 and TXB2 respectively without inducing significant changes in systemic or renal PGI2 production. Simultaneously with the latter effects, the production of TXB2 by spontaneously coagulated whole blood ex vivo is inhibited (>99%) while that of PGE2 and PGF2α is largely increased. Administration of ridogrel causes a three- to five-fold shift to the right of concentration-response curves for U46619 in eliciting platelet aggregation; no tachyphylaxis is observed after 29 days of treatment in this respect. Apart from a reduction of serum uric acid levels with a concomitant increase in urinary uric acid excretion during the first days of treatment, no clinically significant changes in hematological, biochemical, hemodynamic and coagulation parameters occur during the 8 days or 29 days study. The study demonstrates that ridogrel is a potent inhibitor of the systemic as well as renal TXA2 synthase and an antagonist of platelet TXA2/PG endoperoxide receptor in man, covering full activity during 24 h at steady-state plasma level conditions without tachyphylaxis during 29 days of medication. The compound is well tolerated, at least during 1 month of administration.

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Applications of isotope dilution‐mass spectrometry in clinical chemistry, pharmacokinetics, and toxicology

Leenheer

Thienpont

1992

Mass Spectrometry Reviews

109

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Determination of prostaglandins D2, E2, F2α and 6-ketoprostaglandin F1α as well as thromboxane B2 in rat brain by gas chromatography-mass spectrometry

Cited by 8 publications

References 12 publications

Eicosanoids in Rat Brain during Ischemia and Reperfusion—Correlation to DC Depolarization

Eicosanoids in Rat Brain during Ischemia and Reperfusion—Correlation to DC Depolarization

Ridogrel Inhibits Systemic and Renal Formation of Thromboxane A2 and Antagonizes Platelet Thromboxane A2/Prostaglandin Endoperoxide Receptors upon Chronic Administration to Man

Applications of isotope dilution‐mass spectrometry in clinical chemistry, pharmacokinetics, and toxicology

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