Determination of proliferation index in neoplasms using different Ki‐67 equivalent antibodies

Abstract

Paraffin sections from 23 tumours were immunohistochemically stained with the following four Ki-67 equivalent antibodies: monoclonal MIB-1 (DAKO), monoclonal MM1 (Novocastra), polyclonal NCL-Ki-67p (Novocastra), and polyclonal Rah Ki-67 (DAKO). Ki-67 labelling indices were determined by counting in exactly the same area in each case. MIB-1 showed the highest labelling index in 21 of the 23 cases, and the mean MIB-1 index was approximately 30% higher than that of the other antibodies. The differences between MM1, NCL-Ki-67p and Rah Ki-67 were small and non-significant. There was a positive correlation between each of the four antibodies. As these findings may be of importance when the Ki-67 labelling index is used as a criterion for tumour grading or for clinical prognostication, this necessitate identification of the antibody used in every case.

The Prognostic Role of Ki-67/MIB-1 in Cervical Cancer: A Systematic Review with Meta-Analysis

“…In our meta-analysis, the antibody used in IHC to identify Ki-67 protein was not always performed with the same antibody: some studies used anti-MIB-1, some used anti-Ki-67 or MIB-1, and some studies provided no information of antibodies. Two recent studies compared different available Ki-67/MIB-1 equivalent antibodies, showing that the 4 tested Ki-67 equivalent antibodies appeared to make differences in quantitative and qualitative staining feature [ 36 , 37 ] According to these 2 studies, MIB-1 antibody is the most suitable to detect Ki-67 antigen because it has the highest sensitivity and gives the clearest staining when compared to other available antibodies and the use of varied concentrations of the antibody also has an effect on the staining result. With positive nuclear staining for a common standard, only when the tumor cells stained to a certain extent, Ki-67/MIB-1 expression can be considered to be positive.…”

Evaluación por inmunohistoquímica de la expresión de hormonas hipofisiarias y del marcador de proliferación celular Ki-67 en tejido de adenomas causantes de acromegalia

“…Sin embargo, hay que señalar que estas series están compuestas mayoritariamente por adenomas no funcionantes y que no se ha analizado dirigidamente a los adenomas secretores de GH. Además, es importante tener en cuenta al comparar resultados cuantitativos, las diferencias en la sensibilidad de los diferentes kits de anticuerpos para el antígeno Ki-67 25 .…”

Spatial intratumoral heterogeneity of proliferation in immunohistochemical images of solid tumors

“…This may have implications when the proliferation index is used as a criterion for tumor grading or clinical prognostication. 56,57 However, due to the multiscale nature of the lacunarity metric, spatial heterogeneity measurements may not be affected as much since expression is assessed in a distributional manner in 2D space. Still, there might be cases, e.g., G1 or G2 pNENs in which variations can emerge in spatial heterogeneity measurements due to IHC setup.…”