Determination of progestogen concentrations in the vaginal fluid of bitches in oestrus

Prediction of parturition in the bitch using semi‐quantitative ELISA measurement of plasma progesterone concentration

“…Progesterone concentrations may be measured by radioimmunoassay, although commercial test kits designed to measure the concentration of plasma progestogen by enzyme‐linked immunosorbent assay ( elisa ) have recently become available. Both quantitative and qualitative elisa kits have been shown to be useful for detecting the preovulatory rise in progesterone and to be clinically useful for the detection of the optimum mating time (Eckersall and Harvey 1987, Dieleman and Blankenstein 1988, Dobretsberger and others 1988, England and others 1989b, Glossop and others 1989, England 1991, England and Anderton 1992).…”

“…Days in Relation to Parturition FIG 1: Plasma progesterone concentrations measured by radioimmunoassay from 10 bitches commencing approximately 14 days before parturition until 14 days after parturition. Mean (±sem) values are demonstrated by the thick line Dobretsberger and others 1988, England and others 1989b, Glossop and others 1989, England 1991, England and Anderton 1992.…”