Determination of polythiazide and prazosin in human plasma by high-performance liquid chromatography

Dokládalová, J.; Coco, S.J.; Lemke, P.R.; Quercia, G.T.; Korst, J.J.

doi:10.1016/s0378-4347(00)80135-8

Cited by 26 publications

(5 citation statements)

References 4 publications

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“…However, the present method besides being very fast, versatile and tunable, belongs to the green techniques with no use of detrimental materials either for the analyst or the environment. It is worth to note here that several attempts (non electrochemical) in the field of chromatography and spectroscopy have also been devoted to the determination of PRA 7–10. However, these methods suffer from difficulty in sample preparation and steep‐cost which render them intrinsically time consuming and unattractive for use in determination of PRA.…”

Section: Resultsmentioning

confidence: 99%

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Three‐Dimensional Superwetting Mesh Film Based On Graphene Assembly for Liquid Transportation and Selective Absorption

et al. 2013

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A sensitive electrochemical sensor based on in situ modification of graphite electrode via graphene nanosheets (GNs) was developed as a green method for prazosin hydrochloride (PRA) analyses. In this study, GNs were electrochemically synthesized on the surface of graphite electrode via in situ approach and used for the analyses. The proposed sensor showed several advantages such as high sensitivity, low LOD, and excellent repeatability. The oxidation peak current at the optimum analytical conditions using a GNs/graphite electrode at pH 6.0 was linearly dependent on PRA concentration in the range of 0.09–100 µM. A LOD of 0.02 µM and RSD of 3.8 % for 10 µM solution of PRA with a great recovery were obtained.

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Section: Resultsmentioning

confidence: 99%

“…Different methods have been applied to determine PRA such as chromatography 7–9, spectrophotometry 10, voltammetry 11 and radio receptor assay 12. PRA determination has so far been subjected to various methods which among them HPLC seems to be prevail.…”

Section: Introductionmentioning

confidence: 99%

Three‐Dimensional Superwetting Mesh Film Based On Graphene Assembly for Liquid Transportation and Selective Absorption

et al. 2013

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“…Extensive survey of literature few methods has been reported for the simultaneous estimation of prazosin and polythiazide using spectroscopic and chromatographic methods. [5][6][7][8] However, there is no reported for the bioanalytical methods. The main aim of the present study is to develop a precise, sensitive, accurate, selective, reproducible, and rapid bioanalytical technique for the estimation of prazosin and polythiazide in human plasma [9][10][11][12][13] and validated as per ICH guidelines.…”

Section: Original Articlementioning

confidence: 99%

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Dharmamoorthy¹

2020

AJP

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Objective: A new simple and precise stability-indicating bioanalytical reverse-phase high-performance liquid chromatography (RP-HPLC) method was developed and validated for the simultaneous estimation of prazosin and polythiazide in their formulation and in human plasma. Materials and Methods: The developed method was successfully used for assaying drug contents in plasma. Isocratic elution mode was carried at Agilent C18 column (150 mm × 4.6 mm, 5 µm particle size) using 0.01 N disodium hydrogen phosphate pH 5.0:acetonitrile (55:45) as mobile phase at flow rate 1.0 ml min at detection wavelength 265 nm. Celecoxib was taken as an internal standard. The method was validated as per ICH guidelines. Results and Discussion: The present validated method can be successfully applied for the estimation of prazosin and polythiazide in human plasma over the concentration range of 12.5-500 ng/m of prazosin and 6.25-250 ng/ml of polythiazide. The method for the determination of prazosin and polythiazide in human plasma using HPLC detection met the acceptance criteria with respect to selectivity, precision, accuracy, linearity, and recovery. Conclusion: The proposed method is simple, rapid, accurate, precise, and appropriate for pharmacokinetic and therapeutic drug monitoring in the clinical laboratories.

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“…Methods for the analysis of prazosin in biological samples include HPLC [18][19][20][21][22][23][24][25][26][27][28], densitometry [29], voltammetry [14], spectrophotometry [14] and radio-receptor assay [30]. An HPLC method has been reported for determination of terazosin and its impurities [31].…”

Section: Introductionmentioning

confidence: 99%

“…Bakshi et al / Journal of Pharmaceutical and BiomedicalAnalysis 34 (2004) [19][20][21][22][23][24][25][26] …”

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confidence: 99%

Validated specific HPLC methods for determination of prazosin, terazosin and doxazosin in the presence of degradation products formed under ICH-recommended stress conditions

Bakshi

Ojha

Singh

2004

Journal of Pharmaceutical and Biomedical Analysis

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Determination of polythiazide and prazosin in human plasma by high-performance liquid chromatography

Cited by 26 publications

References 4 publications

Three‐Dimensional Superwetting Mesh Film Based On Graphene Assembly for Liquid Transportation and Selective Absorption

Three‐Dimensional Superwetting Mesh Film Based On Graphene Assembly for Liquid Transportation and Selective Absorption

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Validated specific HPLC methods for determination of prazosin, terazosin and doxazosin in the presence of degradation products formed under ICH-recommended stress conditions

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