Determination of Polysorbate 60 in Salad Dressings by Colorimetric and Thin Layer Chromatographic Techniques

Abstract

Methods are described for the quantitation and verification of polysorbate 60 (PS 60) in salad dressing. The sample is partitioned between methylene chloride and water. The methylene chloride extract is further treated by silica gel column chromatography. The isolated PS 60 is complexed with ammonium cobaltothiocyanate and determined spectrophotometrically at 620 nm. Additional evidence indicating the presence of PS 60 is obtained from thin layer chromatographic analysis using the modified Dragendorff reagent for visualization of spots.

Investigating the Molecular Heterogeneity of Polysorbate Emulsifiers by MALDI-TOF MS

“…Since the introduction of the hydrophile-lipophile balance (HLB) system several decades ago, few attempts have been made to further elucidate the nature of polysorbate molecular complex-ity, although many analytical methods have been developed. Techniques such as colorimetry (5,6), infrared spectroscopy (6), and gravimetric determination by precipitation (7,8) focus on qualitative detection of polysorbates in food products. Quantification and rudimentary characterization have been attempted using techniques such as thin-layer chromatography (5,6,9,10), gas chromatography (6,11,12), high-performance liquid chromatography (10,13), OHnegative ion chemical ionization mass spectrometry (14), and recently, an optical chemical sensor method (15).…”

“…Techniques such as colorimetry (5,6), infrared spectroscopy (6), and gravimetric determination by precipitation (7,8) focus on qualitative detection of polysorbates in food products. Quantification and rudimentary characterization have been attempted using techniques such as thin-layer chromatography (5,6,9,10), gas chromatography (6,11,12), high-performance liquid chromatography (10,13), OHnegative ion chemical ionization mass spectrometry (14), and recently, an optical chemical sensor method (15). Unfortunately, none of these methods allows satisfactory separation or resolution of the individual polymer species, nor identification of the components.…”

Rapid and efficient incorporation of tissue factor into liposomes

“…C 12 E 8 and Triton X‐100 concentrations in relipidation mixtures were measured using a colorimetric method adapted from Daniels et al . [14]. Briefly, standards were prepared from PCPS dried as described above and resuspended in HBS spiked with known quantities of C 12 E 8 or Triton X‐100.…”

Characterization of polysorbates by OH- negative ion chemical ionization mass spectrometry

“…Relatively few methods for the determination of polysorbates are available, and they focus primarily on characterization of the POE content, either as free oligomers (III) or bound to the sorbitan (I, II, and IV). Thus, complexation was used for the detection of POE by steric exclusion chromatography (Warner et al, 1979) and in thin-layer chromatographic (TLC) determination of polysorbate 60 in salad dressing (Daniels et al, 1982). Earlier work using TLC included that of (1969) and Hodda (1976).…”

“…An aliquot of the organic layer was evaporated to dryness and the residue partitioned between hexane and acetonitrile. The acetonitrile layer was streaked on a preparative scale TLC plate and developed as described by Daniels et al (1982). The plate was scraped at the appropriate Rf and the scrapings were extracted with methylene chloride and filtered through a Millipore Millex filter (0.5 µ ).…”