Determination of Polyphenol Components of Korean Prostrate Spurge (Euphorbia supina) by Using Liquid Chromatography—Tandem Mass Spectrometry: Overall Contribution to Antioxidant Activity

Abstract

The Korean prostrate spurge Euphorbia supina is a weed that has been used in folk medicine in Korea against a variety of diseases. Nine polyphenols were characterized for this plant by using high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) and the results were compared with the literature data. The individual components were validated using the calibration curves of structurally related external standards and quantified for the first time by using the validated method. Correlation coefficients (r 2) were >0.9907. The limit of detection and limit of quantification of the method were >0.028 mg/L and 0.094 mg/L, respectively. Recoveries measured at 50 mg/L and 100 mg/L were 76.1–102.8% and 85.2–98.6%, respectively. The total amount of the identified polyphenols was 3352.9 ± 2.8 mg/kg fresh plant. Quercetin and kaempferol derivatives formed 84.8% of the total polyphenols. The antioxidant activities of the flavonoids were evaluated in terms of 1,1-diphenyl-2-picrylhydrazyl and 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) radical cation-scavenging activity, and the reducing power showed a dose-dependent increase. Cell viability was effectively suppressed at polyphenol mixture concentrations >250 mg/L.

An aqueous polyphenol extract from Rosa rugosa tea has antiaging effects on Caenorhabditis elegans

“…According to our results, 23 kinds of polyphenols were separated and identified, with quercetin and its glycosides being the principal component, having a relative percentage content of 25.93% in total. Our results were completely in accordance with some previous reports (Mario et al, ; Riffault et al, ; Song et al, ; Zhu et al, ). So, we used quercetin as the positive control in the following experiments of RAP activity determination.…”

Polyphenols fromArtemisia annuaL Inhibit Adhesion and EMT of Highly Metastatic Breast Cancer Cells MDA-MB-231

“…The thirty‐two polyphenols in the KAL were as follows: caffeic acid ( 1 ), syringic aldehyde ( 2 ), dicaffeoylquinic acid isomer ( 3 , 4 , 11 ), Quercetin 3‐O‐galactoside ( 5 ), dicaffeoylquinic acid isomer ( 6 ), mearnsetin 3‐O‐hexoside isomer ( 7 , 10 ), kaempferol 3‐O‐glucoside ( 8 ), quercetin 3‐O‐glucoside ( 9 ), ferulic acid ( 12 ), caffeoylferuloylquinic acid isomer ( 13 , 17–19 ), isorhamnetin 3‐O‐glucoside ( 14 ), diosmetin 7‐O‐glucoside ( 15 ), luteolin 7‐O‐glucoside ( 16 ), diferuloylquinic acid ( 21 ), quercetin ( 22 ), dicaffeoylferuloylquinic acid isomer ( 23 ), 3‐O‐methylquercetagetin ( 24 ), dicaffeoylferuloylquinic acid isomer ( 25 ), luteolin ( 26 ), 8‐methoxykaempferol ( 27 ), 3,5‐dimethoxyquercetagetin ( 28 ), caffeoyldiferuloyl quinic acid ( 29 ), kaempferol ( 30 ), 3,5‐dihydroxy‐6,7,4’‐trimethoxyflavone ( 31 ), and 3,5‐dihydroxy‐6,7,3’,4’‐tetramethoxyflavone ( 32 ). Quercetin and kaempferol derivatives formed 84.8% of the total polyphenols (Song et al, ). Twelve hydroxycinnamic acids ( 1 , 3 , 4 , 6 , 11 , 13 , 17–19 , 21 , 23 , and 25 ) and ten flavonoids ( 7 , 9 , 10 , 14 , 16 , 22 , 26 , and 30–32 ) were previously reported in Chinese, Brazilian, and Italian A. annua (Carbonara et al, ).…”

Polyphenol mixtures of Euphorbia supina the inhibit invasion and metastasis of highly metastatic breast cancer MDA-MB-231 cells

“…Polyphenols from E. supine (PES) were extracted and purified by Professor S.C. Shin as reported in Song et al (10). Briefly, the lyophilized E. supine tissue (10 g) was ground into powder and extracted in ethyl acetate (300 ml) at 80˚c for 20 h, and eluted using a mixture of methanol:dichloromethane (1:5, 25 ml).…”

“…Epidemiological studies have shown that polyphenols reduce the risk of chronic diseases (7,8), and have anti-oxidant, anti-aging, and anti-microbial properties (9). According to Song et al (10), nine polyphenols have been isolated and identified from Korean E. supine, and quercetin and kaempferol derivatives account for 84.8% of the total polyphenols. Therefore, the inhibitory effects of E. supine on cancer growth and metastasis could be expected; however, few studies have been performed to demonstrate this effect.…”