Determination of Polybrominated Biphenyl Residues in Dry Animal Feeds

Fehringer, Norbert V

doi:10.1093/jaoac/58.6.1206

Cited by 6 publications

(5 citation statements)

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“…shown peaks 1 and 2 to be pentabromo, peaks 3, 4, and 5 hexabromo, and peak 6 heptabromobiphenyl (Jacobs, et al, 1976). Most routine analyses of PBB levels in animal tissues or products have been reported based on analysis of only the major hexa-peak, peak 3 (Willett and Irving, 1976; Fries and Marrow, 1975;Fehringer, 1975;Gutenmann and Lisk, 1975). Recovery values for the method described here were calculated for all of the six major peaks.…”

Section: Discussionmentioning

confidence: 99%

Method for extraction, isolation, and detection of free polybrominated biphenyls (PBBs) from plasma, feces, milk, and bile using disposable glassware

Willett¹,

Brumm²,

Williams³

1978

J. Agric. Food Chem.

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A rapid method was developed for the extraction, isolation, and detection of polybrominated biphenyls (PBBs) from plasma, feces, milk, and bile, using disposable glassware. Use of disposable equipment greatly reduced the amount of laboratory background and cross-contamination of samples. The procedure employed a multiple extraction with a mixture of diethyl and petroleum ethers, followed by cleanup on miniature Florisil, silica gel, and sodium sulfate columns. Detection was accomplished by gas chromatography. Recoveries were determined for the six major components of a commercial PBB mixture and were approximately 96% for plasma, 59% for feces, and 98% for milk. The background levels for plasma, feces, and milk were 0.0005, 0.0007, and 0.0007 ppm, respectively, bringing the minimum detectable limits of the major hexabromobiphenyl peak to 0.0010, 0.0014, and 0.0014 ppm on a whole tissue basis.

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Section: Discussionmentioning

confidence: 99%

Method for extraction, isolation, and detection of free polybrominated biphenyls (PBBs) from plasma, feces, milk, and bile using disposable glassware

Willett¹,

Brumm²,

Williams³

1978

J. Agric. Food Chem.

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“…The original identification of PBBs in animal feed by Dr. George F. Fries was performed by gas chromatography (3), and this type of instrumentation has been used in assay methods which were developed subsequently (32)(33)(34)(35). The increasingly stringent FDA guidelines for PBBs in foods and animal feeds have been based upon the sensitivity of these methods, not upon toxicological findings (3).…”

Section: Assaymentioning

confidence: 99%

“…One involves gel-permeation chromatography as a cleanup procedure for the assay of PBBs in extracts of fat from butter, milk and cheese (33). The other method involves cleanup by elution from Florisil columns and was used to determine PBBs in animal feeds (34). These procedures can measure PBBs at levels as low as 7 ppb in dairy products and 3 ppb in dry animal feeds.…”

Section: Assaymentioning

confidence: 99%

Assessment of the hazards of polybrominated biphenyls.

Carlo¹,

Seifter²,

DeCarlo³

1978

Environ Health Perspect

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During their peak use period, PBBs represented under 1% of the total sales of fire retardant chemicals, and very probably would have escaped intensive study if they had not been mixed accidentally with animal feed preparations. Instead, international attention was drawn to PBBs by the state-supervised killing of over 35,000 cattle which had been contaminated with PBBs. Interestingly, low doses of PBBs exert a broad spectrum of toxicological, pharmacological, and biochemical effects despite low acute toxicity. These effects and the intensive bioaccumulation of PBBs derive from their structure and their consequent resistance of biotransformation and high solubility in fat. In rodents, PBBs are teratogenic, immunosuppressive, and potentially carcinogenic. In bovine, rodent, and avian species, PBBs reduce feed intake and induce mixed function oxidases of liver microsomes. The latter effect may be responsible for steroid level changes which underline hormonal toxicities observed in cows, mink, rats, and chickens. The effects of PBBs on humans are controversial, but data suggestive of immunological, skin, and liver disorders continue to accumulate. Concern about the clinical effects of PBBs is heightened by the knowledge that these compounds readily enter the fetus by crossing the placental barrier and can be transferred to newborn children after extensive passage into breast milk.

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“…Fehringer (111) described a new procedure for PBB residues in dry animal feeds. Finely ground feed was packed into a chromatographic column containing Celite and then eluted with methylene chloride.…”

Section: Gas Chromatographymentioning

confidence: 99%

Pesticide residues

Thornburg¹

1977

Anal. Chem.

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Determination of Polybrominated Biphenyl Residues in Dry Animal Feeds

Cited by 6 publications

References 0 publications

Method for extraction, isolation, and detection of free polybrominated biphenyls (PBBs) from plasma, feces, milk, and bile using disposable glassware

Method for extraction, isolation, and detection of free polybrominated biphenyls (PBBs) from plasma, feces, milk, and bile using disposable glassware

Assessment of the hazards of polybrominated biphenyls.

Pesticide residues

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