Determination of platinum traces contamination by graphite furnace atomic absorption spectrometry after preconcentration by cloud point extraction

Chappuy, Mathieu; Caudron, Éric; Bellanger, Agnès; Pradeau, D.

doi:10.1016/j.jhazmat.2009.11.014

Cited by 25 publications

(12 citation statements)

References 25 publications

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“…AAS encompasses the technique of flame atomic absorption spectrometry (FAAS) and flameless atomic absorption spectrometry; the latter mainly includes graphite furnace atomic absorption spectrometry (GFAAS). With higher tolerance to inorganic and organic matrices and higher sensitivity compared with FAAS, GFAAS has been enjoying a high reputation as a mature and sensitive technique used in routine determination of metal contents (Chappuy et al, 2010; Dessuy et al, 2011; Lewen, 2011). With spectrophotometric, fluorescence, electrochemical and quenched phosphorescence detection methods, HPLC is characterized with high specificity and selectivity (Boiteau et al, 2013; Khuhawar & Arain, 2005; Santa, 2013).…”

Section: Introductionmentioning

confidence: 99%

Comparison of different sample preparation methods for platinum determination in cultured cells by graphite furnace atomic absorption spectrometry

Xiao

Huang

Cai

et al. 2017

PeerJ

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BackgroundPlatinum-based agents are widely used in chemotherapy against solid tumors and insufficient intracellular drug accumulation is one of the leading causes of platinum resistance which is associated with poor survival of tumor patients. Thus, the detection of intracellular platinum is pivotal for studies aiming to overcome platinum resistance. In the present study, we aimed to establish a reliable graphite furnace atomic absorption spectrometry (GFAAS)-based assay to quantify the intracellular platinum content for cultured cells.MethodsSeveral most commonly applied cell preparation methods, including 0.2% HNO3, 0.2% Triton X-100, concentrated nitric acid, RIPA combined with concentrated nitric acid and hydroxide, followed by GFAAS for platinum detection were compared in ovarian, cervical and liver cancer cell lines to obtain the optimal one, and parameters regarding linearity, accuracy, precision and sensitivity were evaluated. Influence of other metals on platinum detection and the storage conditions of samples were also determined.ResultsThe treatment of cells with 0.2% HNO3 was superior to other approaches with fewer platinum loss and better repeatability. The recovery rate and precision of this method were 97.3%–103.0% and 1.4%–3.8%, respectively. The average recoveries in the presence of other metals were 95.1%–103.1%. The detection limit was 13.23 ug/L. The recovery rate of platinum remained acceptable even in cell samples stored in −20 °C or −80 °C for two months.DiscussionAfter comparison, we found that 0.2% HNO3 was optimal for intracellular platinum quantification based on GFAAS, which presented values compatible with that of inductively-coupled plasma mass-spectrometry (ICP-MS), and this is partially attributed to the simplicity of this method. Moreover, the assay was proved to be accurate, sensitive, cost-effective and suitable for the research of platinum-based antitumor therapy.

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Section: Introductionmentioning

confidence: 99%

Comparison of different sample preparation methods for platinum determination in cultured cells by graphite furnace atomic absorption spectrometry

Xiao

Huang

Cai

et al. 2017

PeerJ

View full text Add to dashboard Cite

show abstract

“…AAS encompasses the technique of flame atomic absorption spectrometry (FAAS) and flameless atomic absorption spectrometry; the latter mainly includes graphite furnace atomic absorption spectrometry (GFAAS). With higher tolerance to inorganic and organic matrices and higher sensitivity compared with FAAS, GFAAS has been enjoying a high reputation as a mature and sensitive technique used in routine determination of metal contents (Chappuy et al 2010;Dessuy et al 2011;Lewen 2011). With spectrophotometric, fluorescence, electrochemical and quenched phosphorescence detection methods, HPLC is characterized with PeerJ reviewing PDF | (2016:09:13363:1:0:NEW 7 Nov 2016)…”

Section: Introductionmentioning

confidence: 99%

Peer Review #2 of "Comparison of different sample preparation methods for platinum determination in cultured cells by graphite furnace atomic absorption spectrometry (v0.2)"

2017

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Background. Platinum-based agents are widely used in chemotherapy against solid tumors and insufficient intracellular drug accumulation is one of the leading causes of platinum resistance which is associated with poor survival of tumor patients. Thus, the detection of intracellular platinum is pivotal for studies aiming to overcome platinum resistance. In the present study, we aimed to establish a reliable graphite furnace atomic absorption spectrometry (GFAAS)-based assay to quantify the intracellular platinum content for cultured cells. Methods. Several most commonly applied cell preparation methods, including 0.2% HNO 3 , 0.2% Triton X-100, concentrated nitric acid, RIPA combined with concentrated nitric acid and hydroxide, followed by GFAAS for platinum detection were compared in ovarian, cervical and liver cancer cell lines to obtain the optimal one, and parameters regarding linearity, accuracy, precision and sensitivity were evaluated. Influence of other metals on platinum detection and the storage conditions of samples were also determined. Results. The treatment of cells with 0.2% HNO 3 was superior to other approaches with fewer platinum loss and better repeatability. The recovery rate and precision of this method were 97.3%-103.0% and 1.4%-3.8%, respectively. The average recoveries in the presence of other metals were 95.1%-103.1%. The detectionlimit was 13.23 ug/L. The recovery rate of platinum remained acceptable even in cell samples stored in-20˚C or-80˚C for two months. Discussion. After comparison, we found that 0.2% HNO 3 was optimal for intracellular platinum quantification based on GFAAS, which presented values compatible with that of inductively-coupled plasma mass-spectrometry (ICP-MS), and this is partially attributed to the simplicity of this method. Moreover, the assay was proved to be accurate, sensitive, cost-effective and suitable for the research of platinum-based antitumor therapy.

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“…Platinum also finds applications in chemical and glass industries as cladding on account of anticorrosion properties and in electronic industry as material for electrodes, contacts and resistance wires. Another field of application of platinum is the manufacture of jewellery [1][2][3] . It has been shown that platinum in road dusts has increased after the introduction of vehicle catalyst.…”

Section: Introductionmentioning

confidence: 99%

Preconcentration of Platinum Using Multi-Walled Carbon Nanotubes as Solid Phase Extraction Sorbent

Yang¹,

Huang²,

Yang³

2014

Asian J. Chem.

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A solid phase extraction and graphite furnace atomic absorption spectrometry for the determination of platinum with multi-walled carbon nanotubes as sorbent was studied. Platinum are preconcentrated on multi-walled carbon nanotubes in the form of (L2•H3O +)2PtCl6 2ionpairs, where L = heptyl octyl sulfoxide and water was used as eluent. The effects of various parameters such as acidity, flow rate of sample, type of eluent, amount of adsorbent and interfering ions have been studied. The platinum in 100 mL solution can be concentrated to 2 mL, representing an enrichment factor of 50 was achieved. The detection limit and quantification limit of the method were found to be 0.06 and 0.2 µg L-1 , respectively. The analytical results for the certified reference sample (GBW07292) was in a good agreement with the certified value. The relative standard deviation for ten replicate measurements of 1 µg L-1 platinum was 4.5 %. The proposed method was successfully applied to the extraction and determination of trace amount platinum in different samples.

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Determination of platinum traces contamination by graphite furnace atomic absorption spectrometry after preconcentration by cloud point extraction

Cited by 25 publications

References 25 publications

Comparison of different sample preparation methods for platinum determination in cultured cells by graphite furnace atomic absorption spectrometry

Comparison of different sample preparation methods for platinum determination in cultured cells by graphite furnace atomic absorption spectrometry

Peer Review #2 of "Comparison of different sample preparation methods for platinum determination in cultured cells by graphite furnace atomic absorption spectrometry (v0.2)"

Preconcentration of Platinum Using Multi-Walled Carbon Nanotubes as Solid Phase Extraction Sorbent

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