Determination of Platelet Viability

Chao, Francis C.

doi:10.1111/j.1423-0410.1971.tb01815.x

Cited by 3 publications

(1 citation statement)

References 28 publications

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“…ATP is a marker of viable cells as it is rapidly degraded after cell death. The assay is based on the principle that luciferase catalyzes the oxidation of luciferin in the presence of ATP, producing light that is proportional to the amount of ATP present [ 197 ].…”

Section: Cell-based Screening Assays For Novel Anti-inflammatory Drugsmentioning

confidence: 99%

From the Bush to the Brain: Preclinical Stages of Ethnobotanical Anti-Inflammatory and Neuroprotective Drug Discovery—An Australian Example

Kumar

Mathew

Gamage

et al. 2023

IJMS

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The Australian rainforest is a rich source of medicinal plants that have evolved in the face of dramatic environmental challenges over a million years due to its prolonged geographical isolation from other continents. The rainforest consists of an inherent richness of plant secondary metabolites that are the most intense in the rainforest. The search for more potent and more bioavailable compounds from other plant sources is ongoing, and our short review will outline the pathways from the discovery of bioactive plants to the structural identification of active compounds, testing for potency, and then neuroprotection in a triculture system, and finally, the validation in an appropriate neuro-inflammatory mouse model, using some examples from our current research. We will focus on neuroinflammation as a potential treatment target for neurodegenerative diseases including multiple sclerosis (MS), Parkinson’s (PD), and Alzheimer’s disease (AD) for these plant-derived, anti-inflammatory molecules and highlight cytokine suppressive anti-inflammatory drugs (CSAIDs) as a better alternative to conventional nonsteroidal anti-inflammatory drugs (NSAIDs) to treat neuroinflammatory disorders.

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Section: Cell-based Screening Assays For Novel Anti-inflammatory Drugsmentioning

confidence: 99%

From the Bush to the Brain: Preclinical Stages of Ethnobotanical Anti-Inflammatory and Neuroprotective Drug Discovery—An Australian Example

Kumar

Mathew

Gamage

et al. 2023

IJMS

View full text Add to dashboard Cite

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In Vitro Analysis of Platelet Function During Storage of Platelets from Plasmapheresed Donors

et al. 1973

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Several in vitro platelet function assays were examined. Two units of platelets from each of ten volunteer donors were obtained by plasmapheresis, stored three days, and examined by various functional parameters. The second unit of platelets from an individual had generally lower functional parameters than the first, suggesting a subtle physiological change in the donors' circulation between the time of drawing the first and second units. For in vitro comparisons, this difference was overcome by a pooling procedure. Platelet storage was examined at room temperature and at 4 C.

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The Relation Between Response to Hypotonic Stress and the ⁵¹Cr Recovery In Vivo of Preserved Platelets

1974

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Platelet response to hypotonic stres can be used to estimate the SlCr recovery in u k o of liquid-and frceze-preserved platelets. This simple in rtitro test may prove helpful in determining and controlling the quality of preserved platelets. I n our study, the response to hypotonic stress was not related to the "ICr T-W value of the preserved platelets, but was related to the number of irrevcmibly damaged platelets removed within two hours after transfu- sion.A SIMPLE in uilro test for predicting the survival in vivo and hemostatic effectiveness of preserved platelets would be extremely useful in assessing methods of preservation. Suggested in uitro tests for estimating platelet viability include: serotonin uptake,l4. 1 8 . 2 3 response to hypotonic stress,50 99 11, 16-l7-19 ability to promote clot retraction,6,13 platelet adhesion and w egation,3.4. lo. 20.24 morphology,2; platelet factor 320 and measurement of platelet enzymes such as aminopeptidase, nucleoside diphosphokinase, 3-phosphoglycerate kinase, and enolase, in the supernatant of the platelet concentrate.7.*. 21.23 We report here on the "Cr survival and the response to hypotonic stress of liquid-antl freezepreserved human platelets.

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Determination of Platelet Viability

Cited by 3 publications

References 28 publications

From the Bush to the Brain: Preclinical Stages of Ethnobotanical Anti-Inflammatory and Neuroprotective Drug Discovery—An Australian Example

From the Bush to the Brain: Preclinical Stages of Ethnobotanical Anti-Inflammatory and Neuroprotective Drug Discovery—An Australian Example

In Vitro Analysis of Platelet Function During Storage of Platelets from Plasmapheresed Donors

The Relation Between Response to Hypotonic Stress and the ⁵¹Cr Recovery In Vivo of Preserved Platelets

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Determination of Platelet Viability

Cited by 3 publications

References 28 publications

From the Bush to the Brain: Preclinical Stages of Ethnobotanical Anti-Inflammatory and Neuroprotective Drug Discovery—An Australian Example

From the Bush to the Brain: Preclinical Stages of Ethnobotanical Anti-Inflammatory and Neuroprotective Drug Discovery—An Australian Example

In Vitro Analysis of Platelet Function During Storage of Platelets from Plasmapheresed Donors

The Relation Between Response to Hypotonic Stress and the 51Cr Recovery In Vivo of Preserved Platelets

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The Relation Between Response to Hypotonic Stress and the ⁵¹Cr Recovery In Vivo of Preserved Platelets