Determination of plasmin in milk using QCM and ELISA methods

Poturnayová, Alexandra; Szabo, Katalin; Tatarko, Marek; Hucker, Attila; Kocsis, Robert; Hianik, Tibor

doi:10.1016/j.foodcont.2020.107774

Cited by 13 publications

(16 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, in our recent work we demonstrated that the AFM technique can confirm the cleavage of the β-casein layer by another proteaseplasmin. 44 Most recently we performed comparative analysis of the application of the acoustic and ELISA methods for the determination of the cleavage of β-casein by plasmin. While both methods revealed similar sensitivity, the acoustic method has the advantage of measuring protease activity, which is not possible by ELISA that detects only the protease concentration 44…”

Section: Layer Thickness Analysismentioning

confidence: 99%

Analysis of trypsin activity at β-casein layers formed on hydrophobic surfaces using a multiharmonic acoustic method

Spagnolo

Muckley

Ivanov

et al. 2022

Analyst

Self Cite

View full text Add to dashboard Cite

show abstract

Section: Layer Thickness Analysismentioning

confidence: 99%

Analysis of trypsin activity at β-casein layers formed on hydrophobic surfaces using a multiharmonic acoustic method

Spagnolo

Muckley

Ivanov

et al. 2022

Analyst

Self Cite

View full text Add to dashboard Cite

show abstract

“…Plasmin was detected by exploiting its proteolytic function to cleave β-casein, where the use of the complete protein as a QCM biosensor coating was more successful than the use of a cleavable peptide. In contrast to an antibody coating, which would lead to the detection of the complete plasmin concentration, the use of the enzyme’s substrate as a sensor coating allows for the specific detection of the active plasmin [ 80 , 81 ]. Immunoglobulin G (IgG) is another minor component in milk.…”

Section: Application Of Baw and Saw Sensors And Biosensors In Milk Me...mentioning

confidence: 99%

Bulk and Surface Acoustic Wave Biosensors for Milk Analysis

Länge

2022

Biosensors

View full text Add to dashboard Cite

Milk and dairy products are common foods and, therefore, are subject to regular controls. Such controls cover both the identification and quantification of specific components and the determination of physical parameters. Components include the usual milk ingredients, mainly carbohydrates, proteins, and fat, and any impurities that may be present. The latter range from small molecules, such as drug residues, to large molecules, e.g., protein-based toxins, to pathogenic microorganisms. Physical parameters of interest include viscosity as an indicator of milk gelation. Bulk and surface acoustic wave sensors, such as quartz crystal microbalance (QCM) and surface acoustic wave (SAW) devices, can principally be used for both types of analysis, with the actual application mainly depending on the device coating and the test format. This review summarizes the achievements of acoustic sensor devices used for milk analysis applications, including the determination of physical liquid parameters and the detection of low- and high-molecular-weight analytes and microorganisms. It is shown how the various requirements resulting from the respective analytes and the complex sample matrix are addressed, and to what extent the analytical demands, e.g., with regard to legal limits, are met.

show abstract

“…The QCM biosensor with AT-cut quartz has been used for the detection of various disease biomarkers found in human serum, namely the carcinoembryonic antigen (CEA); prostate-specific antigen (PSA); CD63 positive exosomes; tuberculosis biomarker; Dengue fever biomarker (non-structural protein-1 (NS1)); pancreatic disease biomarker (trypsin); hepatitis B core antigen (HBcAg); protein biomarker for malaria (PfHRP-2); and human immunodeficiency virus (HIV) biomarker (HIV-1 glycoprotein 41 (gp41), HIV-1 p24 antigen) [75,97,98,111,112,[120][121][122][123][124][125][126][127][128]. Apart from the disease biomarkers, QCM has also been used for the detection of various proteins and amino acids in human serum, such as tryptophan (Trp), immunoglobulin (IgG), hemoglobin, and plasmin (PLA), which act as early disease diagnosis markers [123,[129][130][131][132]. QCM has also been used for the study of the affinity interaction of protein biomolecules [133,134].…”

Section: (I) Proteins and Biomolecular Detectionmentioning

confidence: 99%

“…The technique employed here further lowered the LOD of the QCM biosensor to 7.8 pg/mL. The protease activity of some of the target protein species, such as trypsin or plasmin, can be utilized as the detection principle using the QCM [75,122,132,136]. The bioreceptors used for the detection are either an artificially synthesized peptide chain (Pcc) or proteins, such as ß-casein, instead of antibodies.…”

Section: (I) Proteins and Biomolecular Detectionmentioning

confidence: 99%

“…A synthetic peptide-based QCM sensor with AT-cut quartz operating at 7.995 MHz was reported for the detection of trypsin (biomarker for pancreatitis) and exhibited an LOD of 8.6 ng/mL [75]. A similar protease activity-based detection principle was used for sensing proteolysis in milk, involving plasmin (PLA) using the QCM [131,132,136,137].…”

Section: (I) Proteins and Biomolecular Detectionmentioning

confidence: 99%

See 1 more Smart Citation

Acoustic Biosensors and Microfluidic Devices in the Decennium: Principles and Applications

Nair

Teo

2021

Micromachines

View full text Add to dashboard Cite

Lab-on-a-chip (LOC) technology has gained primary attention in the past decade, where label-free biosensors and microfluidic actuation platforms are integrated to realize such LOC devices. Among the multitude of technologies that enables the successful integration of these two features, the piezoelectric acoustic wave method is best suited for handling biological samples due to biocompatibility, label-free and non-invasive properties. In this review paper, we present a study on the use of acoustic waves generated by piezoelectric materials in the area of label-free biosensors and microfluidic actuation towards the realization of LOC and POC devices. The categorization of acoustic wave technology into the bulk acoustic wave and surface acoustic wave has been considered with the inclusion of biological sample sensing and manipulation applications. This paper presents an approach with a comprehensive study on the fundamental operating principles of acoustic waves in biosensing and microfluidic actuation, acoustic wave modes suitable for sensing and actuation, piezoelectric materials used for acoustic wave generation, fabrication methods, and challenges in the use of acoustic wave modes in biosensing. Recent developments in the past decade, in various sensing potentialities of acoustic waves in a myriad of applications, including sensing of proteins, disease biomarkers, DNA, pathogenic microorganisms, acoustofluidic manipulation, and the sorting of biological samples such as cells, have been given primary focus. An insight into the future perspectives of real-time, label-free, and portable LOC devices utilizing acoustic waves is also presented. The developments in the field of thin-film piezoelectric materials, with the possibility of integrating sensing and actuation on a single platform utilizing the reversible property of smart piezoelectric materials, provide a step forward in the realization of monolithic integrated LOC and POC devices. Finally, the present paper highlights the key benefits and challenges in terms of commercialization, in the field of acoustic wave-based biosensors and actuation platforms.

show abstract

Determination of plasmin in milk using QCM and ELISA methods

Cited by 13 publications

References 29 publications

Analysis of trypsin activity at β-casein layers formed on hydrophobic surfaces using a multiharmonic acoustic method

Analysis of trypsin activity at β-casein layers formed on hydrophobic surfaces using a multiharmonic acoustic method

Bulk and Surface Acoustic Wave Biosensors for Milk Analysis

Acoustic Biosensors and Microfluidic Devices in the Decennium: Principles and Applications

Contact Info

Product

Resources

About