Determination of plasma non-esterified fatty acids and triglyceride fatty acids by gas chromatography of their methyl esters after isolation by column chromatography on silica gel

Separation and Quantitation of Phospholipids and Lysophospholipids by High-Performance Liquid Chromatography

“…The reproducibility of the normal-phase HPLC method is excellent, and recovery of PPL and lyso-PPL from HPLC is in excess of 75%. Individual PPL and lyso-PPL are available for quantitation via organic phosphate measurement (16) or acylgroup analysis (17) or for separation and characterization of individual molecular species by reverse-phase HPLC (7,13,18,19).…”

“…Individual PPL and lyso-PPL peaks were identified by comparison of retention time to standards (phosphatidylcholine, PC; phosphatidylethanolamine, PE; phosphatidylserine, PS; phosphatidylinositol, PI; cardiolipin, CL; sphingomyelin, SM; lysophosphatidylcholine, LPC; lysophosphatidyethanolamine; lysophosphatidylserine, LPS; lysophosphatidylinositol; dilysocardiolipin, LCL). PPL and lyso-PPL chromatographic peaks were collected and analyzed for organic phosphate (16) or acyl-group composition (17). Cardiolipin was characterized by reversed-phase HPLC to separate individual molecular species as described below.…”

“…Acyl-group composition and content was performed using alkaline hydrolysis and the formation of fatty acid methyl esters by derivitization using 2,2-dimethoxypropane, with subsequent gas chromatography using total ion current mass selective detection (17).…”

Derivatization of fatty acids and its application for conjugated linoleic acid studies in ruminant meat lipids

“…This, perhaps, contributes to reluctance amongst laboratories to use it as a general procedure in the preparation of fatty acid methyl esters. There was an interesting report concerning unreliable estimates of serum non-esterified fatty acids with the use of diazomethane; 78 however, a subsequent report 79 was able to clarify that solvolysis of serum lipids took place to increase the free fatty acid content. In fact, numerous publications have used diazomethane quite satisfactorily for the methylation of their fatty acids.…”

Rapid determination of C12–C26 non‐derivatized fatty acids in human serum by fast gas chromatography

“…Direct analysis of LCFAs without derivatization should be very interesting, especially for those laboratories where a great number of analyses are carried out or quick results are needed, since short analysis time, low cost, and operational convenience can be achieved. However, quantitative measurement of fatty acids without derivatization has been reported only for short chain organic acids with a chain shorter than 18 carbon atoms [11,18]. Minimum run times in chromatographic analysis have attracted great interest for many years [19].…”