Determination of plasma Levetiracetam level by Liquid Chromatography-Tandem Mass Spectrometry (LC-MS-MS) and its application in pharmacokinetics studies in neonates

Determination of levetiracetam in human plasma by online heart‐cutting liquid chromatography: Application to therapeutic drug monitoring

“…Studies for the determination of levetiracetam in human plasma [13][14][15][16][17] are sparse. LC with ultraviolet detection [13,14], LC-photodiode array detection [15], GC-MS [16], and LC-tandem MS [17][18][19][20][21][22][23] methods have been developed for the determination of levetiracetam. Some of these studies require complex sample preparation procedures, such as SPE and evaporation under a nitrogen stream [15][16][17][18][19], whereas others required protein precipitation [13,14,[20][21][22][23].…”

“…Various analytical techniques (HPLC, CE, and GC) are typically used for the separation of drugs, and among them, HPLC is the most common approach [11,12]. Despite the importance, there are few analytical methods reported for the determination of levetiracetam in human plasma [13][14][15][16][17][18][19][20][21][22][23]. Further, online heart-cutting LC methods have not been reported for the determination of levetiracetam in plasma.…”

Development and validation of a liquid chromatography‐tandem mass spectrometry method with triple‐stage fragmentation to determine levetiracetam in epileptic patient serum and its application in therapeutic drug monitoring

“…To date, many different analytical techniques have been applied to determine LEV in human plasma/serum, including HPLC with UV detection [6, 8, 17], GC‐MS [18, 19], LC‐MS [5, 20, 21], CE [22, 23]. Compared with these analytical techniques, LC‐MS/MS method has become the common choice for bioanalysis of LEV due to its high specificity, sensitivity, and excellent reproducibility.…”