Determination of plasma lactic acid concentration and specific activity using high-performance liquid chromatography

Bleiberg, B.; Steinberg, Jacob J.; Katz, Sam; Wexler, John; LeJemtel, Thierry H.

doi:10.1016/0378-4347(91)80167-b

Cited by 23 publications

(13 citation statements)

References 33 publications

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“…According to the method of Bleiberg et al (1991), a reversed-phase highperformance liquid chromatography (RP-HPLC) separation method with UV detection was developed for the detection of lactate and glucose. All analyses were carried out on a Waters 600 controller pump, a Rheodyne 7295 injector with a 10 L loop, equipped with a Waters 2996 photodiode array detector.…”

Section: Glucose and Lactate Levels In Str Hip And Mpfcmentioning

confidence: 99%

Effects of early life permethrin exposure on spatial working memory and on monoamine levels in different brain areas of pre-senescent rats

et al. 2013

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a b s t r a c tPesticide exposure during brain development could represent an important risk factor for the onset of neurodegenerative diseases. Previous studies investigated the effect of permethrin (PERM) administered at 34 mg/kg, a dose close to the no observable adverse effect level (NOAEL) from post natal day (PND) 6 to PND 21 in rats. Despite the PERM dose did not elicited overt signs of toxicity (i.e. normal body weight gain curve), it was able to induce striatal neurodegeneration (dopamine and Nurr1 reduction, and lipid peroxidation increase). The present study was designed to characterize the cognitive deficits in the current animal model. When during late adulthood PERM treated rats were tested for spatial working memory performances in a T-maze-rewarded alternation task they took longer to choose for the correct arm in comparison to age matched controls. No differences between groups were found in anxiety-like state, locomotor activity, feeding behavior and spatial orientation task. Our findings showing a selective effect of PERM treatment on the T-maze task point to an involvement of frontal cortico-striatal circuitry rather than to a role for the hippocampus. The predominant disturbances concern the dopamine (DA) depletion in the striatum and, the serotonin (5-HT) and noradrenaline (NE) unbalance together with a hypometabolic state in the medial prefrontal cortex area. In the hippocampus, an increase of NE and a decrease of DA were observed in PERM treated rats as compared to controls. The concentration of the most representative marker for pyrethroid exposure (3-phenoxybenzoic acid) measured in the urine of rodents 12 h after the last treatment was 41.50 g/L and it was completely eliminated after 96 h.

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Section: Glucose and Lactate Levels In Str Hip And Mpfcmentioning

confidence: 99%

Effects of early life permethrin exposure on spatial working memory and on monoamine levels in different brain areas of pre-senescent rats

et al. 2013

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“…The quantitative data obtained are given in Table 3. Also given in this table are the results obtained by the HPLC method [10]. The HPLC conditions included separation on a reversed-phase column (octadecylsilane) and an isocratic buffer (30% ACN in water).…”

Section: Clinical Applicationmentioning

confidence: 99%

“…Simonides and coworkers [9] described a method using HPLC in which the esterification of lactate with the UV-absorbing compound a-p-dibromoacetophenone was followed by separation and quantitation of the ester by reversed-phase HPLC. In another report [10], lactate was detected in the UV range by derivatization with a-bromoacetophenone. Biosensors are the other choice for lactate assays [11][12][13], which are also mostly based on LDH or lactate oxidase (LOD).…”

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confidence: 97%

Quantification of lactate in synovia by microchip with contactless conductivity detection

Song

Chen

et al. 2013

Analytical Biochemistry

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“…The use of chromatographic separation methods has also been reported. Gas chromatography is possible after derivatization to render lactate volatile [7], but also determination by HPLC requires derivatization to allow UV-or fluorescence detection as lactate is a poor UV absorber [8][9][10][11].…”

Section: Introductionmentioning

confidence: 99%

Quantification of plasma lactate concentrations using capillary electrophoresis with contactless conductivity detection

et al. 2011

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The quantification of plasma lactate and evaluation of the lactate threshold by CE with capacitively coupled contactless conductivity is demonstrated. The only sample preparation needed was deproteinization with a ACN/methanol mixture. A solution of 10 mmol/L 2-morpholinoethanesulfonic acid monohydrate, 10 mmol/L DL-histidine, 70 μmol/L hexadecyltrimethylammonium bromide, pH 6.0 was found suitable as running buffer. Linearity was achieved for the concentration range of 10-1000 μmol/L with a correlation coefficient of 0.9994. The limit of detection (3 S/N) was determined as 3.2 μmol/L. Intra- and inter-day variabilities were less than 7% RSD. The suitability of the method could be demonstrated by analyzing various clinical samples, where the results correlated satisfactorily with those of an established enzymatic method.

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Determination of plasma lactic acid concentration and specific activity using high-performance liquid chromatography

Cited by 23 publications

References 33 publications

Effects of early life permethrin exposure on spatial working memory and on monoamine levels in different brain areas of pre-senescent rats

Effects of early life permethrin exposure on spatial working memory and on monoamine levels in different brain areas of pre-senescent rats

Quantification of lactate in synovia by microchip with contactless conductivity detection

Quantification of plasma lactate concentrations using capillary electrophoresis with contactless conductivity detection

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