Determination of plasma choline by high-performance liquid chromatography with a postcolumn enzyme reactor and electrochemical detection

Fossati, Tiziano; Colombo, Michelle L.; Castiglioni, Carlo Andrea; Abbiati, G.

doi:10.1016/0378-4347(94)00070-0

Cited by 31 publications

(26 citation statements)

References 20 publications

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“…Plasma-free choline and plasma total homocysteine assay HPLC-electrochemical detection of plasma-free choline was performed according to a method adapted from Fossati et al (26) . After protein precipitation, samples were centrifuged to remove proteins.…”

Section: Tissue Preparationmentioning

confidence: 99%

Plasma choline concentration varies with different dietary levels of vitamins B₆, B₁₂and folic acid in rats maintained on choline-adequate diets

et al. 2011

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Choline is an important component of the human diet and is required for the endogenous synthesis of choline-containing phospholipids, acetylcholine and betaine. Choline can also be synthesised de novo by the sequential methylation of phosphatidylethanolamine to phosphatidylcholine. Vitamins B 6 , B 12 and folate can enhance methylation capacity and therefore could influence choline availability not only by increasing endogenous choline synthesis but also by reducing choline utilisation. In the present experiment, we determined whether combined supplementation of these B vitamins affects plasma choline concentration in a rat model of mild B vitamin deficiency which shows moderate increases in plasma homocysteine. To this end, we measured plasma choline and homocysteine concentrations in rats that had consumed a B vitamin-poor diet for 4 weeks after which they were either continued on the B vitamin-poor diet or switched to a B vitamin-enriched diet for another 4 weeks. Both diets contained recommended amounts of choline. Rats receiving the B vitamin-enriched diet showed higher plasma choline and lower plasma homocysteine concentrations as compared to rats that were continued on the B vitamin-poor diet. These data underline the interdependence between dietary B vitamins and plasma choline concentration, possibly via the combined effects of the three B vitamins on methylation capacity.

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Section: Tissue Preparationmentioning

confidence: 99%

Plasma choline concentration varies with different dietary levels of vitamins B₆, B₁₂and folic acid in rats maintained on choline-adequate diets

et al. 2011

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“…This is probably explained by the inconvenient methods for the determination of the quaternary ammonium compounds choline and betaine. Various methods have been reported for the measurement of plasma free choline, including radioenzymatic assays (14,15 ), gas chromatography (8,16 ), HPLC with electrochemical detection (17 ), and very recently, HPLCmass spectrometry (MS) (18 ). The radioenzymatic assay is cumbersome with variable recovery (8 ), whereas the chromatographic assays require complex extraction or derivatization procedures.…”

confidence: 99%

Determination of Choline, Betaine, and Dimethylglycine in Plasma by a High-Throughput Method Based on Normal-Phase Chromatography–Tandem Mass Spectrometry

Holm¹,

Ueland²,

Kvalheim³

et al. 2003

Clinical Chemistry

289

186

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Background:The quaternary ammonium compounds, choline and betaine, and dimethylglycine (DMG) reside along a metabolic pathway linked to the synthesis of neurotransmitters and membrane phospholipids and to homocysteine remethylation and, therefore, folate status. Lack of a convenient, high-throughput method for the determination of these compounds has prevented population-based studies of their possible associations with lifestyle, nutrition, and chronic diseases. Methods: Serum or plasma samples were deproteinized by mixing with three volumes of acetonitrile that contained d 9 -choline and d 9 -betaine as internal standards. We used a normal-phase silica column for the separation of choline (retention time, 2.8 min), betaine (1.3 min), DMG (1.15 min), and internal standards, which were detected as positive ions by tandem mass spectroscopy in the multiple-reaction monitoring mode, using the molecular transitions m/z 104360 (choline), m/z 113369 (d 9 -choline), m/z 118359 (betaine), m/z 127368 (d 9 -betaine), and m/z 104358 (DMG). Results: For all three metabolites, the assay was linear in the range 0.4 -400 mol/L, and the lower limit of the detection (signal-to-noise ratio ‫؍‬ 5) was <0.3 mol/L. The within-and between-day imprecision (CVs) was 2.1-7.2% and 3.5-8.8%, respectively. The analytical recovery was 87-105%. The fasting plasma concentrations (median, 25th-75th percentiles) were 8.0 (7.0 -9.3) mol/L for choline, 31.7 (27.0 -41.1) mol/L for betaine, and 1.66 (1.30 -2.02) mol/L for DMG in 60 healthy blood donors. In individuals who had eaten a light breakfast, plasma concentrations of all three metabolites

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“…It is the same caution that led to a relatively short incubation time (less than 240 min). In human blood, the choline concentration is about 10 -12 µM and ethanolamine is about 2.5 µM 7 . In order to assess the match of choline and the ethanolamine pathways in in vitro conditions, we chose an identical final concentration of precursors (2.5 µM for each).…”

Section: Discussionmentioning

confidence: 99%

Uptake and kinetic properties of choline and ethanolamine in <i>Plasmodium falciparum</i>

Ahiboh¹,

Djaman²,

Yapi³

et al. 2008

Trop. J. Pharm Res

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Determination of plasma choline by high-performance liquid chromatography with a postcolumn enzyme reactor and electrochemical detection

Cited by 31 publications

References 20 publications

Plasma choline concentration varies with different dietary levels of vitamins B₆, B₁₂and folic acid in rats maintained on choline-adequate diets

Plasma choline concentration varies with different dietary levels of vitamins B₆, B₁₂and folic acid in rats maintained on choline-adequate diets

Determination of Choline, Betaine, and Dimethylglycine in Plasma by a High-Throughput Method Based on Normal-Phase Chromatography–Tandem Mass Spectrometry

Uptake and kinetic properties of choline and ethanolamine in <i>Plasmodium falciparum</i>

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Determination of plasma choline by high-performance liquid chromatography with a postcolumn enzyme reactor and electrochemical detection

Cited by 31 publications

References 20 publications

Plasma choline concentration varies with different dietary levels of vitamins B6, B12and folic acid in rats maintained on choline-adequate diets

Plasma choline concentration varies with different dietary levels of vitamins B6, B12and folic acid in rats maintained on choline-adequate diets

Determination of Choline, Betaine, and Dimethylglycine in Plasma by a High-Throughput Method Based on Normal-Phase Chromatography–Tandem Mass Spectrometry

Uptake and kinetic properties of choline and ethanolamine in <i>Plasmodium falciparum</i>

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Plasma choline concentration varies with different dietary levels of vitamins B₆, B₁₂and folic acid in rats maintained on choline-adequate diets

Plasma choline concentration varies with different dietary levels of vitamins B₆, B₁₂and folic acid in rats maintained on choline-adequate diets