Determination of plasma catecholamines via condensation with diphenylethylenediamine: Simplification of the procedure

Hušek, Petr; Malíková, J.; Herzogová, Gertruda

doi:10.1016/s0378-4347(00)82198-2

Cited by 8 publications

(3 citation statements)

References 13 publications

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“…Epinephrine (normal range, 0.10-0.80 nmol/L) was measured by high-performance liquid chromatography (HPLC) with fluorescence detection after isolation of catecholamines from plasma by extraction and derivatization with 1,2-diphenylethylenediamine using an in-house method (19). Glucose (normal range, 3.90-5.80 mmol/L) was evaluated in venous plasma (glucose oxidase method, Beckmann analyser, Fullerton, CA, USA).…”

Section: Laboratory Assaysmentioning

confidence: 99%

Insulin sensitivity and counter-regulatory hormones in hypothyroidism and during thyroid hormone replacement therapy

Stanická

Vondra²,

Pelikánová³

et al. 2005

Clinical Chemistry and Laboratory Medicine (CCLM)

111

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We examined insulin sensitivity and secretion, together with the levels of selected glucoregulatory hormones, in 15 female patients with severe hypothyroidism (H) and during subsequent thyroid hormone replacement therapy (HRT) using the euglycaemic hyperinsulinaemic clamp technique. Insulin action, as evaluated by glucose disposal, the insulin sensitivity index, and fasting post-hepatic insulin delivery rate were established. The basal levels of insulin, C-peptide and counter-regulatory hormones were measured in basal condition. In H, glucose disposal (p<0.01), the insulin sensitivity index (p<0.01) and post-hepatic insulin delivery rate (p<0.05) were significantly lower than during HRT. No significant changes in the levels of fasting insulin and C-peptide were observed. The levels of counter-regulatory hormones in patients with H were significantly higher than during HRT (glucagon, p<0.05; epinephrine, p<0.01; cortisol, p<0.05; growth hormone, p<0.05). In H, an inverse correlation between insulin sensitivity and insulin secretion was observed (p<0.05). Cortisol was the most important factor affecting the variability of insulin sensitivity values, regardless of thyroid function (p=0.0012). In conclusion, H altered both insulin sensitivity and the levels of selected counter-regulatory hormones. The situation was restored by HRT, as manifested not only by normalisation of insulin sensitivity, secretion and levels of glucoregulatory hormones, but also by improvement of their relationships.

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Section: Laboratory Assaysmentioning

confidence: 99%

Insulin sensitivity and counter-regulatory hormones in hypothyroidism and during thyroid hormone replacement therapy

Stanická

Vondra²,

Pelikánová³

et al. 2005

Clinical Chemistry and Laboratory Medicine (CCLM)

111

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“…Plasma adrenaline and noradrenaline were assayed by high performance liquid chromatography (HPLC) with fluorometric detection after treatment with 1,2-diphenylethylene-diamine, as previously described. 31 Glycerol was determined in plasma using a bioluminescence technique (see 'Measurement of adipocyte lipolysis' section). Blood glucose and plasma NEFA concentrations were measured with a glucose-oxidase technique (Biotrol, Paris, France) and an enzymatic procedure (Wako, Unipath, Dardilly, France), respectively.…”

Section: Exercise-induced Lipolysis In Obese Menmentioning

confidence: 99%

Effect of a long-duration physical exercise on fat cell lipolytic responsiveness to adrenergic agents and insulin in obese men

et al. 2002

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OBJECTIVE:The aim of the study was to investigate whether a long-lasting bout of exercise modifies the lipolytic b-and antilipolytic-a 2 -adrenergic effect and the antilipolytic effect of insulin in obese subjects. DESIGN: Biopsies of abdominal subcutaneous adipose tissue were performed before and immediately after 2 h exercise (at 50% of VO 2max ) on an ergometric bicycle. SUBJECTS: Nine healthy obese male subjects (mean age 38.0 AE 3.5 y; mean body mass index (BMI) 35.6 AE 3.9 kg=m 2 ) were included in the experiment. METHODS: The lipolytic responsiveness to adrenaline, isoprenaline (b-adrenergic agonist), UK-14304 (a 2 -adrenergic agonist) and insulin was studied in the isolated fat cell obtained by biopsies of subcutaneous adipose tissue from the peri-umbilical region before and after exercise. RESULTS: After exercise, an increase was observed in spontaneous lipolytic rate, and in the lipolytic effect of isoprenaline, but no modification in the lipolytic action of adrenaline. Antilipolytic effects of UK-14304 and insulin were not changed by the single bout of exercise. CONCLUSION: A single bout of long-term exercise increased the responsiveness of adipose tissue to b-adrenergic stimulation of lipolysis in obese subjects.

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“…Samples were stored on ice until centrifuged at 3,400 rev min ¡1 for 15 min at 4°C within 60 min of collection. Serum was stored at ¡85°C until completion of all trials, at which time all samples from all participants were analysed in duplicate by Xuorometric high-pressure liquid chromatography method (Husek et al 1990). …”

Section: Beveragesmentioning

confidence: 99%

Effect of carbohydrate ingestion on exercise performance and carbohydrate metabolism in persons with spinal cord injury

et al. 2009

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Carbohydrate ingestion during exercise and as a pre-exercise bolus improves exercise performance in able-bodied athletes. Little is known about the potential for carbohydrate ingestion to improve exercise performance in athletes with spinal cord injury (SCI), nor the potential physiological limitations of such a practice resulting from an SCI. Therefore, this study investigated the effects of carbohydrate ingestion on exercise performance in physically active and athletic persons with SCI. Six participants with complete SCI (neurological level of lesion ranging from C(6) to T(7)) and normal glucose tolerance were studied twice during 60 min of arm cranking at 65% of peak oxygen consumption followed by a 20-min time trial with the ingestion of either a carbohydrate drink (CHO trial: 0.5 g CHO kg(-1) body weight in 500 ml) or placebo (PLA trial) applied in a double-blind counter-balanced manner. The participants with tetraplegia had sufficient neurological function to permit voluntary arm-cranking exercise. There was no difference in time-trial performance between CHO and PLA trials (P > 0.05). The results suggest that carbohydrate ingestion in persons with SCI does not improve exercise performance.

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Determination of plasma catecholamines via condensation with diphenylethylenediamine: Simplification of the procedure

Cited by 8 publications

References 13 publications

Insulin sensitivity and counter-regulatory hormones in hypothyroidism and during thyroid hormone replacement therapy

Insulin sensitivity and counter-regulatory hormones in hypothyroidism and during thyroid hormone replacement therapy

Effect of a long-duration physical exercise on fat cell lipolytic responsiveness to adrenergic agents and insulin in obese men

Effect of carbohydrate ingestion on exercise performance and carbohydrate metabolism in persons with spinal cord injury

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