Determination of plasma amino acids in small samples with the use of Dansyl-chloride

Arola, Lluı́s; Palou, Andreu; Herrera, Emílio; Alemany, M.

doi:10.1016/s0300-9084(76)80121-6

Cited by 21 publications

(10 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In spite of the fact that administration of the ob protein resulted in no significant changes in tissue LPL activity, a tendency for the activity values to be lower is observed in both adipose tissue and skeletal muscle. Since adipose tissue and skeletal muscle represent about 50% of body weight in the rat [39], a small decrease in LPL activity could be responsible for the observed increase in circulating triacylglycerols. To further investigate this possibility, we decided to examine the tissue uptake of an exogenous triacylglycerol load (Table 4 ).…”

Section: Resultsmentioning

confidence: 98%

Short‐term effects of leptin on lipid metabolism in the rat

et al. 1998

View full text Add to dashboard Cite

In this study, we have examined the short-term effects of leptin on lipid metabolism in the rat. Acute leptin administration induced hypertriglyceridaemia (31% increase in plasma triacylglycerols) which was not associated with changes in lipoprotein lipase activity in white adipose tissue. Surprisingly, leptin administration did not induce any changes in the lipogenic rate in either white adipose tissue or liver. Leptin administration caused a decreased tissue uptake of exogenous IR C-triacylglycerols. These data suggest that leptin induces important changes in lipid uptake in adipose tissue and skeletal muscle which could be responsible for the observed hypertriglyceridaemia.z 1998 Federation of European Biochemical Societies.

show abstract

Section: Resultsmentioning

confidence: 98%

Short‐term effects of leptin on lipid metabolism in the rat

et al. 1998

View full text Add to dashboard Cite

show abstract

“…Aliquots of plasma were deproteinized with cold acetone (Arola, Herrera and Alemany 1977) and the supernatants were used for determination of individual amino acids (Arola, Palou, Herrera and Alemany 1976) by means of their derivatization with Dns-Cl ( 14 C), thin layer chromatography separation and evaluation of radioactivity present in individual spots (Arola et al 1976). Tests for significant differences between the means were done using the Student's t test.…”

Section: Methodsmentioning

confidence: 99%

Plasma Amino Acids in Hypothyroid and Hypertyroid Rats

Remesar¹,

Arola²,

Palou³

et al. 1981

Horm Metab Res

Self Cite

View full text Add to dashboard Cite

Plasma amino acid concentrations, together with other metabolic parameters were determined in thyroidectomized rats treated with daily injections of saline (hypothyroid), and 250 M9/kg L-T 4 (hypothyroid). Data were compared with sham-operated controls. There is a general increase in plasma amino acid concentrations in hyperthyroidism, a limited increase only in several amino acid concentrations in hypothyroid rats as compared with controls, and a considerable difference between the plasma aminograms of both groups. Amino acid homeostasis seems to be subject to greater modification in hyperthyroidism than in hypothyroidism.

show abstract

“…Accurate micromethods for the determination of amino acids and protein in small tissue samples are required if investigations in this area are to be pursued in the common laboratory animals under controlled conditions. The adaptation of simple available methodology (Arola et al, 1976(Arola et al, , 1977 has enabled us to investigate this problem.…”

Section: Almentioning

confidence: 99%

Plasma amino acid concentrations in pregnant rats and in 21-day foetuses

Palou¹,

Arola²,

Alemany³

1977

Biochemical Journal

View full text Add to dashboard Cite

Plasma amino acid concentrations were determined in virgin female rats, in pregnant rats (12 and 21 days after impregnation) and in 21-day foetuses. The total amino acid concentration in plasma decreases significantly with pregnancy, being lower at 12 than at 21 days. Alanine, glutamine+glutamate and other 'gluconeogenic' amino acids decrease dramatically by mid-term, but regain their original concentrations at the end of the pregnancy. With most other amino acids, mainly the essential ones, the trend is towards lower concentrations which are maintained throughout pregnancy. These data agree with known nitrogen-conservation schemes in pregnancy and with the important demands on amino acids provoked by foetal growth. In the 21-day foetuses, concentrations of individual amino acids are considerably higher than in their mothers, with high plasma foetal/maternal concentration ratios, especially for lysine, phenylalanine and hydroxy-proline, suggesting active protein biosynthesis and turnover. All other amino acids also have high concentration ratios, presumably owing to their requirement by the foetuses for growth. Alanine, glutamine+glutamate, asparagine+aspartate, glycine, serine and threonine form a lower proportion of the total amino acids in foetuses than in the virgin controls or pregnant rats, probably owing to their role primarily in energy metabolism in the adults. The results indicate that at this phase of foetal growth, the placental amino acid uptake is considerable and seems to be higher than immediately before birth.

show abstract

Determination of plasma amino acids in small samples with the use of Dansyl-chloride

Cited by 21 publications

References 27 publications

Short‐term effects of leptin on lipid metabolism in the rat

Short‐term effects of leptin on lipid metabolism in the rat

Plasma Amino Acids in Hypothyroid and Hypertyroid Rats

Plasma amino acid concentrations in pregnant rats and in 21-day foetuses

Contact Info

Product

Resources

About