Determination of plant and enterolignans in human serum by high-performance liquid chromatography with tandem mass spectrometric detection

Smeds, Annika; Hakala, Kristo; Hurmerinta, Teija T.; Kortela, Liisi; Saarinen, Niina M.; Mäkelä, Sari

doi:10.1016/j.jpba.2005.12.036

Cited by 32 publications

(48 citation statements)

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“…END and ENL were determined by HPLC-MS/MS in the mouse serum samples after enzymatic hydrolysis and solid-phase extraction according to a previously described method [39]. The method was slightly modified by taking 50 ll of serum instead of 600 ll and 290 units of b-glucuronidase/sulphatase dissolved in 0.5 ml of 10 mM of sodium acetate buffer (pH 5.0) for each sample (for the enzymatic hydrolysis).…”

Section: T3-l1 Adipocyte Differentiation Assaymentioning

confidence: 99%

Oilseeds ameliorate metabolic parameters in male mice, while contained lignans inhibit 3T3-L1 adipocyte differentiation in vitro

et al. 2014

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Purpose and background The focus was directed to the study of two of the most lignan-rich food sources: sesame and flaxseeds. Recent epidemiological and experimental evidences suggesting that these foods may improve metabolic functions underlying metabolic syndrome (MetS). Methods To characterize the effect of these oilseeds on metabolic functions, we conducted an experimental study aimed at preventing adiposity and metabolic imbalance in a mouse model of high-fat diet (HFD)-induced MetS. Statistical analysis was performed by two-way analysis of variance test followed by post hoc Bonferroni analysis. Results We studied the effect of the oilseeds sesame and flaxseed on metabolic parameters in mice on a HFD. When the HFD was integrated with 20 % of sesame or flaxseed flours, the mice showed a decrease in body fat, already at day 15, from time 0. The size of the adipocytes was smaller in epididymal fat, liver steatosis was inhibited, and insulin sensitivity was higher in mice on the supplemented diets. The supplemented diets also resulted in a significant increase in the serum levels of the lignan metabolites enterodiol and enterolactone compared with the controls. The expression of genes associated with the inflammatory response, glucose metabolism, adipose metabolism and nuclear receptor were altered by the oilseed-supplemented diets. Some of the most abundant lignans in these oilseeds were studied in 3T3-L1 preadipocyte cells and were effective in inhibiting adipocyte differentiation at the minimal dose of 1 nM. Conclusions The consumption of sesame and flaxseed may be beneficial to decrease metabolic parameters that are generally altered in MetS.

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Section: T3-l1 Adipocyte Differentiation Assaymentioning

confidence: 99%

Oilseeds ameliorate metabolic parameters in male mice, while contained lignans inhibit 3T3-L1 adipocyte differentiation in vitro

et al. 2014

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“…The health effects of lignans depend on both the amount consumed and the bioavailability. Although numerous lignans have been identified, it is not known which of them are converted to enterolignans [33,34]. For about two decades, only secoisolariciresinol and matairesinol were known to be precursors of enterolignans.…”

Section: Lignan Content In Fermented and Untreated Plant Productsmentioning

confidence: 99%

The Influence of Fermentation on the Content of Alkylresorcinols and Lignans in Plant Products

Bartkienė¹,

Skabeikytė²,

Krunglevičiūtė³

et al. 2015

TOBIOTJ

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Abstract:The aim of this study was to evaluate the influence of solid state fermentation (SSF) and submerged fermentation (SmF) with bacteriocin-like inhibitory substances (BLIS) producing lactic acid bacteria (LAB) (Pediococcus acidilactici, Lactobacillus sakei and Pediococcus pentosaceus) on the content of alkylresorcinols (ARs) and lignans in plant products (barley bran, pea fiber, and lupine seeds). Lignans analysis was performed by HPLC-MS/MS, and alkylresorcinols content was evaluated by GC/MS.We found that with the experimentally tested LAB, under SSF conditions more organic acids were produced and in most cases a higher count of the LAB was found in SSF samples, compared to the SmF samples.The matairesinol content was increased by using fermentation (from 7.9 to 35.4 % in pea fiber, from 33.2 to 81.5 % in lupine seeds, and from 5.9 to 74.9 % in barley bran), and in most cases a higher content of matairesinol was found in the SSF samples. The content of secoisolariciresinol in the fermented samples was found to be higher, in comparison to the untreated samples. It was found that the total lignans content in the pea fiber had a strong correlation with the amylase activity (R=0.7908; P=0.0177).Our results suggested that the total ARs content in pea fiber, lupine seeds, and barley bran was 267 μg/g; 1757 μg/g, and 1488 μg/g, respectively, and by using the LAB fermentation, the ARs content was reduced by 40 to 73 %, by 10 to 77 %, and by 24 to 74 %, respectively.We conclude that by using the LAB fermentation, the concentration of lignans in plant products could be increased, but the ARs content could be reduced, and the proper conditions should be selected for the fermentation, in order to prevent possible losses of these biologically active compounds.

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“…The samples were stored at 220°C until analyzed using HPLC-MS/MS with a method previously described. 18 MCF-7 cell culture. MCF-7 human breast cancer cells (The American Type Culture Collection, Manassas, VA), were maintained in Dulbecco's minimum essential medium/F12 (Gibco BRL, Paisley, Scotland) containing 2 times charcoal-stripped heat inactivated FCS (Gibco BRL) and 1% antibiotic-antimycotic solution for 1-2 days prior to the start or the E2 and lignan treatments.…”

Section: Effects Of Lariciresinol On Mammary Cancersmentioning

confidence: 99%

“…15,16 The latest studies have revealed that lariciresinol is one of the most abundant plant lignans in foods consumed in Europe and North America 5,6 and that a significant portion of the total lignan intake is due to this compound. 17 Lariciresinol is bioavailable as it is found in human serum 18 and urine. 19,20 Furthermore, it was identified as the main serum lignan in some women consuming their habitual (Western) diet in Finland.…”

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Dietary lariciresinol attenuates mammary tumor growth and reduces blood vessel density in human MCF‐7 breast cancer xenografts and carcinogen‐induced mammary tumors in rats

Saarinen

Wärri

Dings

et al. 2008

Intl Journal of Cancer

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Lariciresinol is a dietary lignan that accounts for a significant portion of the total phytoestrogen intake from Western foods. Recent epidemiological studies suggest that high dietary intake of lignans and lariciresinol is associated with reduced breast cancer risk. However, no causal relationship between lariciresinol intake and breast cancer development has been established. In this study, we investigated for the first time the effects and possible mechanisms of action of lariciresinol on hormone responsive mammary cancer in vivo in dimethylbenz [a]anthracene induced mammary cancer in rats, and in human MCF-7 breast cancer xenografts in athymic mice. For tumor bearing rats, lariciresinol (3 or 15 mg/kg of body weight) or vehicle was administered p.o. daily for 9 weeks. For E2-maintained ovariectomized athymic mice bearing orthotopic MCF-7 tumors, control diet (AIN-93G) or lariciresinol containing diet (AIN-93G supplemented with 20 or 100 mg of lariciresinol/kg of diet) was administered for 5 weeks. In both models, lariciresinol administration inhibited the tumor growth and tumor angiogenesis. In MCF-7 cells, enterolactone significantly inhibited the E2-stimulated VEGF secretion. Moreover, in MCF-7 xenografts, lariciresinol administration enhanced tumor cell apoptosis and increased estrogen receptor beta expression. Lariciresinol and its further metabolites secoisolariciresinol, enterodiol and enterolactone were found in serum of both rats and athymic mice confirming a similar lignan metabolism pattern as in humans. These findings indicate conceivable importance of dietary lignan lariciresinol in inhibition of breast cancer development.

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Determination of plant and enterolignans in human serum by high-performance liquid chromatography with tandem mass spectrometric detection

Cited by 32 publications

References 46 publications

Oilseeds ameliorate metabolic parameters in male mice, while contained lignans inhibit 3T3-L1 adipocyte differentiation in vitro

Oilseeds ameliorate metabolic parameters in male mice, while contained lignans inhibit 3T3-L1 adipocyte differentiation in vitro

The Influence of Fermentation on the Content of Alkylresorcinols and Lignans in Plant Products

Dietary lariciresinol attenuates mammary tumor growth and reduces blood vessel density in human MCF‐7 breast cancer xenografts and carcinogen‐induced mammary tumors in rats

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