Determination of piribedil in pharmaceutical formulations by micellar electrokinetic capillary chromatography

Abstract

A fast and simple micellar electrokinetic capillary chromatographic method was developed for the analysis of piribedil in pharmaceutical formulations. The effects of buffer concentration, buffer pH, sodium dodecyl sulphate (SDS) concentration, organic modifier, applied voltage and injection time were investigated. Optimum results were obtained with a 50 mM borate buffer at pH 8.0 containing 50 mM SDS by using a fused silica capillary (50 microm internal diameter, 72 cm effective length). The sample was injected hydrodynamically for 4 s at 50 mbar pressure and the applied voltage was +30 kV. The detection wavelength was set at 205 nm. Diflunisal was used as an internal standard. The analysis was performed at 25 degrees C and the total run time was 14 min. The method was suitably validated with respect to linearity range, limit of detection and quantification, precision, accuracy, specificity and robustness. The linear calibration range was 5-100 microg mL(-1) and the limit of detection was determined as 1 microg mL(-1). The method developed was successfully applied to the determination of piribedil in pharmaceutical formulations. The results were compared with a spectrophotometric method reported in the literature and no significant difference was found statistically.

Determination of some drugs used against neurodegenerative disorders by micellar electrokinetic chromatography with running buffer containing SDS

“…Since the solute migration time in CE depends on its ionisation [14][15][16][17][18], we investigated the influence of the buffer pH on the migration time (Figure 1). For this purpose, four buffer compositions of different pH from 6 to 9 were selected.…”

“…Sodium dodecylsulphate (SDS) is a basic surfactant in many MEKC experiments [14][15][16][17]. This was the main reason for choosing this surfactant for our research.…”

Enhancing Nose- To- Brain Delivery of Piribedil: Development of a Nanosuspension Dispersed in Nasal in-Situ Gelling System

“…This improved attention to Piribedil suggests its potential for increased clinical application in the future [17][18][19]. Numerous approaches have been investigated to enhance the solubility of Piribedil, including the utilization of micelles [20], the development of solid lipid micro and nanoparticles [21], and the creation of hybrid nanoparticles using lecithin and chitosan [22].…”

Implementation of Quality by Design Methodology in Development and Validation of a New Stability-Indicating, Reverse Phase High-Performance Liquid Chromatography Method for the Rapid Estimation of Piribedil in Piribedil Prolonged Release Tablets

“…The liquid chromatography methods for estimating piribedil and its metabolite in plasma were also reported [9,10] . An analytical method for estimating piribedil in pharmaceutical formulations by micellar electrokinetic capillary chromatography was also published [11] . In addition to these analytical methods, few more research articles are reported for formulation development and dissolution enhancement [12][13][14][15] .…”