Determination of phytoestrogens in traditional medicinal herbs using gas chromatography–mass spectrometry

Lee, Sangeun; Jung, Byung Hwa; Kim, Sun Yeou; Chung, Bong Chul

doi:10.1016/j.jnutbio.2004.01.007

Cited by 42 publications

(25 citation statements)

References 26 publications

(26 reference statements)

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“…In Fig. 5, it is shown the fragmentation peaks characteristic of DAID (383 and 398 m/z), GEN (399 and 471 m/z), and BIO-A (413 and 428 m/z) (Lee et al 2004;Silveira 2007).…”

Section: Resultsmentioning

confidence: 99%

“…The gas chromatographic system consisted in a Varian CP 3800 apparatus equipped with a VF-5ms type capillary column (30m×0.25 mm i.d., df: 0.25 μm) connected to an ion trap mass spectrometer (Varian Saturn 2200). The GC-MS analytical procedure used for the current estuarine water samples was based in several published protocols (Lee et al 2004;Ballesteros et al 2006;Shareef et al 2006) and was adjusted to the current samples accordingly to Silveira (2007). Thus, as the majority of the EDCs analysed (Fig.…”

Section: Chromatographic System and Main Methodological Characteristicsmentioning

confidence: 99%

“…Thus, as the majority of the EDCs analysed (Fig. 2) contain hydroxyl groups in a steric hindrance position, the MSTFA was the ideal silylation reagent to simultaneous derivatization of all hydroxyl group to prepare the trimethylsilyl (TMS) derivatives (Lee et al 2004;Shareef et al 2006). Data acquisition was obtained using the selected ion monitoring (SIM) mode.…”

Section: Chromatographic System and Main Methodological Characteristicsmentioning

confidence: 99%

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Distribution of endocrine disruptors in the Mondego River estuary, Portugal

Ribeiro

Pardal

Martinho

et al. 2008

Environ Monit Assess

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Recent studies in the Mondego River estuary demonstrated signs of pollution in the area, but the nature of the contamination remains unexplored. Because there seems to be any studies in that zone checking for the simultaneous presence of xenoestrogenic endocrine disrupting compounds, either of animal (estradiol and estrone), vegetal (daidzein, genistein and biochanin A), pharmaceutical (17α-ethynylestradiol) or industrial (bisphenol A, 4-octylphenol, 4-nonylphenol) origins, the main objective of this study was to investigate their presence, in every year season and at eight points along the estuarine gradient. For this propose, water samples (2 L) were collected in high and low tides, preconcentrated in the Oasis HLB cartridges and cleaned in silica cartridges before their analysis by HPLC-DAD and GC-MS. The current data showed the absence of contamination by animal or pharmaceutical estrogens and by alkylphenols, but demonstrated the presence of high levels of phytoestrogens (up to 1

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“…In Fig. 5, it is shown the fragmentation peaks characteristic of DAID (383 and 398 m/z), GEN (399 and 471 m/z), and BIO-A (413 and 428 m/z) (Lee et al 2004;Silveira 2007).…”

Section: Resultsmentioning

confidence: 99%

Section: Chromatographic System and Main Methodological Characteristicsmentioning

confidence: 99%

Section: Chromatographic System and Main Methodological Characteristicsmentioning

confidence: 99%

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Distribution of endocrine disruptors in the Mondego River estuary, Portugal

Ribeiro

Pardal

Martinho

et al. 2008

Environ Monit Assess

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“…Radix clematidis is commonly used in traditional Chinese medicine for rheumatoid arthritis, gout, lower back and limb pain, strokes, headaches, congestion, promotion of urination, menstrual complications, and tonsillitis. Radix clematidis extract (RCE) possess high concentrations of pharmaceutical active isoflavonoids, including quercetin and kaempferol (1). RCE has been found to induce suppression of the nuclear factor-κB (NF-κB) signaling pathway (2,3).…”

Section: Introductionmentioning

confidence: 99%

Radix clematidis extract inhibits TPA-induced MMP-9 expression by suppressing NF-κB activation in MCF-7 human breast cancer cells

et al. 2011

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Abstract. Matrix metalloproteinase-9 (MMP-9), which degrades the extracellular matrix (ECM), plays an important role in breast cancer pathogenesis. Previously, we reported that the Radix clematidis extract (RCE) inhibits MMP expression by suppressing the nuclear factor-κB (NF-κB) pathway. The purpose of the present study was to investigate the effects of RCE on 12-O-tetradecanoyl phorbol-13-acetate (TPA)-induced MMP-9 expression and cell invasion in MCF-7 cells. The toxicity of RCE was determined by MTT assay. MMP-9 expression was determined by real-time PCR, zymography and Western blot analysis. NF-κB activation was assayed by electrophoretic mobility shift assay (EMSA). Results showed that the expression of MMP-9 and cell invasion in response to TPA was increased, whereas TPA-induced MMP-9 expression and cell invasion was decreased by RCE. Moreover, RCE suppressed NF-κB activation in TPA-treated MCF-7 cells. Thus, RCE is a potent inhibitor of TPA-induced MMP-9 expression and markedly blocks the NF-κB pathway in MCF-7 cells.

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“…폐경 이후 골다공증의 예방 및 치료를 위하여 호르몬대체요법과 alendronate 및 raloxifene 등이 사용되고 있다 [4]. 파이토에스트로겐(phytoestrogens)은 식물 유래의 비스테 로이드성 화합물 [6]로 에스트로겐과 유사한 생물학적 활성을 가져 골손실, 기억상실, 심혈관계 질환과 같은 만성 질환을 막는 중요한 역할을 한다는 보고가 있다 [26]. 이로 인해 해조류 와 같은 천연물에서 에스트로겐과 유사한 활성을 확인하는 연구가 진행되고 있다 [15].…”

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The Effects of Medicinal Herbs Extracts on Estrogen-like Activities and Osteoblast Proliferation and Differentiation

김미향¹,

김보경²,

김재덕³

et al. 2017

Journal of Life Science

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The purpose of this study was to investigate the effect of 3 types of medicinal herbs (Glycyrrhizae radix, Astragali radix and Dioscorea rhizoma) extracts on estrogen-like activities, proliferation and differentiation in osteoblast. Human breast cancer cell line MCF7 was transfected using an estrogen responsive luciferase reporter plasmid for measure the estrogen-like activity. Estrogen-like activities of extracts were in the range of 1.11~5.73 fold to that of negative control. The extract of G. radix showed the strongest estrogen-like activities. The estrogen-like activities of 50 and 500 μg/ml extracts of G. radix were similar to that of 10 -8 and 10 -7 M standard solution (17β-estradiol), respectively. G. radix extract showed no cytotoxicity against osteoblast MC3T3-E1 cells at 1~1,000 μg/ml. The extract of A. radix showed no significant proliferation of osteoblast. However, the extract of G. radix and D. rhizome showed maximum 148% and 133% proliferation effects. The extract of G. radix also increased alkaline phosphatase activity and the maximum was 122% at 100 μg/ml compared to that of control. The nodule formation by the method of the Alizarin red S staining increased compared to control. These results suggest that G. radix is able to perform the bone formation and prevent osteoporosis.Key words : Estrogen-like activity, luciferase, medicinal herbs, menopausal women, osteoblast *Corresponding authorTel : +82-51-999-5624, Fax : +82-51-999-5636 E-mail : slee@silla.ac.kr This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. 한약재는 오랜 기간 동안 임상적 경험을 토대로 하여 많은

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Determination of phytoestrogens in traditional medicinal herbs using gas chromatography–mass spectrometry

Cited by 42 publications

References 26 publications

Distribution of endocrine disruptors in the Mondego River estuary, Portugal

Distribution of endocrine disruptors in the Mondego River estuary, Portugal

Radix clematidis extract inhibits TPA-induced MMP-9 expression by suppressing NF-κB activation in MCF-7 human breast cancer cells

The Effects of Medicinal Herbs Extracts on Estrogen-like Activities and Osteoblast Proliferation and Differentiation

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