Determination of phosphorus in phosphorylated deoxyribonucleotides using capillary electrophoresis and high performance liquid chromatography hyphenated to inductively coupled plasma mass spectrometry with an octopole reaction cell

Analysis of mono-phosphate nucleotides as a potential method for quantification of DNA using high performance liquid chromatography–inductively coupled plasma-mass spectrometry

“…The chromatographic method was developed from that of Profrock et al [5], by altering the conditions to improve separation and changing the mobile phase from ammonium acetate to triethylamine acetate to improve compatibility with ICP-MS and using a C 18 column, with high mechanical strength due to a unique organo-silica grafting process making it compatible with high aqueous loading. All separations were made using a Phenomenex Gemini NX column (150x2.1mm, 5µm) at a flow rate of 0.20 mL/min and the column was kept at a constant 25 o C. Isocratic separations were made using TEAA (5mM, pH 6.5) as the mobile phase and samples were injected onto the column at a volume of 2µL.…”

“…Elemental analysis is preferred over other techniques as it offers lower detection limits, a larger dynamic range, a response that is to a first approximation independent of molecular form, and potentially more accurate quantification at low concentrations by isotope dilution analysis. Both ICP-MS and ICP-OES have been utilised for the quantification of nucleotides, DNA, oligonucleotides and DNA adducts [3][4][5][6][7][8][9][10][11][12][13][14][15]. The quantification of oligonucleotides has been achieved by two different methods by Yang et al [4] and Donald et al [7], with the latter group digesting the oligonucleotides to mono-nucleosides and quantifying by isotope dilution electrospray mass spectrometry (ES-ID-MS), and comparing that value to the total 31 P signal obtained from ICP-OES and the gravimetric value.…”

Inductively coupled plasma- and glow discharge plasma-sector field mass spectrometry : Part II. Applications

“…Unfortunately, phosphorus and sulfur are known to be elements which are difficult to determine via plasma-based spectrochemistry, since they are characterized by a high first ionization potential, low ionization efficiencies and suffer from overlap with polyatomic ions such as 15 This situation gets even worse in the case of HPLC coupling with organic solvents used as the liquid phase. [311][312][313] For these applications ICP-SFMS is the method of choice because it resolves the spectral interferences from the analyte.…”

Nano-particle labelling of nucleic acids for enhanced detection by inductively-coupled plasma mass spectrometry (ICP-MS)

“…Most elements in the periodic table can be ionised in the ICP source including biologically 15 important elements such as P and S and as a result, ICP-MS has been increasingly employed in the analysis of nucleic acids and proteins. [1][2][3] ICP-MS provides complementary information to conventional organic MS and has many additional advantages. 2,4,5 Firstly, ICP-MS offers very low limits of detection, which 20 generally range from pg/l -µg/l depending on the analyte.…”