Determination of phenolic compounds, in vitro antioxidant activity and characterization of secondary metabolites in different parts of Passiflora cincinnata by HPLC-DAD-MS/MS analysis

Abstract

Ethanol extracts of different parts of P. cincinnata were obtained by maceration. The total phenolic and flavonoid contents were evaluated. The antioxidant activities were determined by β-carotene-linoleic acid bleaching test, 2,2-diphenyl-1-picrylhydrazil (DPPH), and 2,2'-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical scavenging. The crude ethanol stem extract showed the highest amount of total polyphenols (45.53 mg gallic acid equivalent/g) while the highest total flavonoid contents (1.42 mg of quercetin equivalent/g) were observed in the leaf extract. The lowest IC 50 (25.65 μg/ml) by the DPPH method was observed for the stem extract. The ABTS method showed a significant antioxidant activity for all investigated extracts.The secondary metabolite composition of ethanol extracts was assessed by HPLC-DAD-MS/MS analysis, leading to the identification of fourteen secondary metabolites in P. cincinnata extracts. These results showed the potentiality of this species as a source of phenolic compounds and antioxidants.

Systematic analysis of chemical profiles of Sophorae tonkinensis Radix et Rhizoma in vitro and in vivo using UPLC‐Q‐TOF‐MSE

“…Flavonoids are also one of the major components in S. tonkinensis . It is worth mentioning that the flavonoids have significant biological activities, such as anticancer (Lu et al, 2016), antioxidant (Aderogba et al, 2007; Leal et al, 2020), anti‐gastric ulcer (Sasajima et al, 1978), and antiviral (Dai et al, 2019). In addition, some studies have found that flavonoids in S. tonkinensis have potential toxicity (Liu, Zhu, et al, 2017).…”

Short communication: Potential use of passion fruit (Passiflora cincinnata) as a biopreservative in the production of coalho cheese, a traditional Brazilian cheese

“…This plant species is well adapted to local cultivation conditions in northeastern Brazil and highly resistant to pests and diseases (Araujo, 2007). Passiflora cincinnata has been found to have aromatic metabolites, substances with antimicrobial activity and antioxidant potential, and is able to reduce nociceptive and inflammatory events in mice (de Lavor et al, 2018;Leal et al, 2018;Siebra et al, 2018). These beneficial properties are important features when evaluating P. cincinnata as a potential producer of novel bioactive compounds that could be exploited by the food and pharmaceutical industries (Joana Gil-Chávez et al, 2013).…”

“…Passion fruit is widely used in Brazil, especially in the northeastern region, for preparing beverages and in popular medicine, because of its potential applica-tion as a sedative, antioxidant, and anxiolytic (Dhawan et al, 2004;Leal et al, 2018). It is well known that extracts of certain plants can increase the quality and sensory attributes of foods because of their antimicrobial and antioxidant activity and the presence of pleasant aromatic compounds and coloring characteristics; however, these extracts must be characterized before proper application (Tayel et al, 2013).…”

“…Terpenoids and phenolic compounds are the usual substances obtained from plants with antimicrobial activity, and these substances are reportedly produced by P. cincinnata (Costa et al, 2016;Leal et al, 2018). Phenolic substances, in particular, have potential application in food systems as antioxidants: low concentrations of phenolic acids act negatively on microbial enzymatic activity and high concentrations cause protein denaturation (Hassanien et al, 2014).…”

Center for Studies and Research of Medicinal Plants (NEPLAME): a brief historic, major advances and perspectives

“…Foram identificados 14 flavonoides, previamente descritos em outras espécies de Passiflora. 52 O extrato etanólico bruto das folhas também apresentou atividade antinociceptiva e anti-inflamatória in vivo. 53…”