Determination of Phenolic Compounds and Antioxidant Activity in Leaves from Wild Rubus L. Species

Oszmiański, Jan; Wojdyło, Aneta; Nowicka, Paulina; Teleszko, Mirosława; Cebulak, Tomasz; Wolanin, Mateusz

doi:10.3390/molecules20034951

Cited by 60 publications

(83 citation statements)

References 26 publications

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“…It was also found that Rubus L. leaves have high antioxidant capacity and total phenolic content compared to their fruit tissues (Wang, Lin, 2000). Oszmiański et al (2015) showed that phenolic compounds of high molecular weight, primarily ellagitannins, are major contributors to antioxidant activity in leaves of Rubus L. species. According to the results of this study investigated conditions are suitable for production of extracts with high content of phenolic antioxidants.…”

Section: Resultsmentioning

confidence: 98%

Effect of extraction conditions on phenolic compounds from blackberry leaves extracts

Salević¹,

Kalušević²,

Lević³

et al. 2017

FoodBalt

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Blackberry leaves have been used as a tea substituent in many herbal mixtures. Medicinal properties of this plant material are related to a high level of components with antioxidant activity, such as phenolic compounds. The aim of the research was to evaluate the effect of different extraction conditions on the content of phenolic compounds and antioxidant activity of blackberry leaves extracts. In this study, blackberry leaves extracts were produced by an aqueous extraction procedure. Different extraction conditions: water temperature (40 and 80 °C) and extraction time (15 and 30 min) were investigated. The blackberry leaves extract prepared by applying higher temperature (80 °C) and longer time (30 minutes) was characterized by the highest contents of total phenolic compounds (1534.15 mg gallic acid equivalents L -1 ), flavonoids (715 mg quercetin equivalents L -1 ) and flavan-3-ols (28.21 mg (+)-catechin L -1 ). Also, this extract expressed the highest antioxidant activity in terms of the ferric reducing ability of plasma (27.33 mmol Trolox equivalents L -1 ) and generation of 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) radical cation (1.47 mmol Trolox equivalents L -1 ). The obtained results indicated that the produced extracts are a rich source of phenolic compounds with high antioxidant activity. Among investigated conditions, the use of water heated to higher temperature (80 °C) during prolonged time (30 min) is the most optimal procedure for the extraction of phenolic compounds with antioxidant activity from blackberry leaves. Further research is needed to determine the exact phenolic profile and their bioavailability, as well as to develop new functional food ingredients or nutraceuticals containing blackberry leaves extracts.

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Section: Resultsmentioning

confidence: 98%

Effect of extraction conditions on phenolic compounds from blackberry leaves extracts

Salević¹,

Kalušević²,

Lević³

et al. 2017

FoodBalt

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“…The highest total antioxidant activity in the leaves of the analysed R. idaeus cultivars determined with the FRAP and Folin-Ciocalteu methods was found for 'Radziejowa' (Chwil and Kostryco 2018). The concentration of phenolic compounds in the leaf extracts from the different Rubus species was significantly correlated with the antioxidant activity (Oszmiański et al 2015;Om et al 2016). Szymanowska et al (2018) reported antioxidant and anti-inflammatory properties of phenolic compounds and anthocyanins contained in R. idaeus fruit extracts.…”

Section: Histochemistrymentioning

confidence: 89%

Histochemical assays of secretory trichomes and the structure and content of mineral nutrients in Rubus idaeus L. leaves

Chwil

Kostryco

2019

Protoplasma

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Leaves of Rubus idaeus are a raw material, ingredients of herbal blend, and a source of antioxidants. There are no data concerning histochemistry of trichomes, and little is known about the leaf structure of this species. The aim of this study was to determine the histochemistry of active compounds and the structure of glandular trichomes, micromorphology, anatomy, and ultrastructure of leaves as well as content of elements. To determine the histochemistry of glandular trichomes, different chemical compounds were used. The leaf structure was analysed using light, scanning, and transmission electron microscopes. The content of elements was determined with atomic absorption spectrometry, and the microanalysis of the epidermis ultrastructure was carried out with a transmission electron microscope equipped with a digital X-ray analyser. In glandular trichomes, polyphenols, terpenes, lipids, proteins, and carbohydrates were identified. The main elements in the ultrastructure of the epidermis were Na, Mo, Se, Ca, and Mg. In dry matter of leaves, K, Mg, Ca, P, and Fe were dominant. Infusions from leaves are safe for health in terms of the Cd and Pb concentrations. Leaves can be a valuable raw material. Non-glandular trichomes prevent clumping of mixed raw materials in herbal mixtures.

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“…polyphenols and flavonoid) have antidiabetic potential, so identifying and evaluating medicinal plants by antioxidant and antidiabetic properties can be used for expanding new therapeutic against diabetes disease. As Rubus species contain polyphenolic compounds, 22,24 in this study we investigate the effect of R.anatolicus leaf extract on glucose metabolism.…”

Section: Discussionmentioning

confidence: 99%

“…The antioxidant effect of R. anatolicus leaves is due to high phenolic compounds, which have antioxidant and free radical scavenging activities. [21][22][23][24] Free radicals and oxidative stress that results from overproduction of reactive oxygen species (ROS) are closely associated with a number of disease states such as hyperglycemia and metabolic disorders including diabetes mellitus. 25 Therefore, it is reasonable that dietary antioxidants, especially polyphenolic compounds that present in wild plants can neutralize free radicals and oxidative stress.…”

Section: Introductionmentioning

confidence: 99%

<p>Effect of <em>Rubus anatolicus</em> Leaf Extract on Glucose Metabolism in HepG2, CRI-D2 and C2C12 Cell Lines</p>

Safarzad¹,

Marjani²,

Jazi³

et al. 2020

DMSO

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The aim of this study was to assess the effects of Rubus anatolicus on glucose metabolism in HepG2, CRI-D2 and C2C12 cell lines. Materials and Methods: R. anatolicus was collected in Golestan province, Iran. Three different cell lines HepG2 (human liver cell), CRI-D2 (mice pancreatic cell) and C2C12 (rat myoblast) were used for cell culture experiments. Cell viability was measured using MTT assay. Cells were treated with various concentrations of the extract (6.25-400 μg/mL) and then the extracellular glucose level and intracellular glycogen content were measured using colorimetric methods. The insulin level of the culture medium was measured using the ELISA method. Results: Our findings showed that R. anatolicus extract enhances glucose uptake and consumption by all three cell lines. The R. anatolicus extract exposure also elevated cellular glycogen content in HepG2 and C2C12 cells (for 200 and 100 μg/mL) significantly. We found a significant increase in glucose uptake and consequently higher stimulation of insulin secretion in CRI-D2 cell pancreatic cells treated with R. anatolicus extract. Conclusion: The R. anatolicus appears to activate glucose uptake and cellular glycogen synthesis probably by activating the glycogenesis or inhibition of glycogenolysis pathways. The extract enhances insulin secretion in the pancreatic cells by increased glucose uptake.

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Determination of Phenolic Compounds and Antioxidant Activity in Leaves from Wild Rubus L. Species

Cited by 60 publications

References 26 publications

Effect of extraction conditions on phenolic compounds from blackberry leaves extracts

Effect of extraction conditions on phenolic compounds from blackberry leaves extracts

Histochemical assays of secretory trichomes and the structure and content of mineral nutrients in Rubus idaeus L. leaves

<p>Effect of <em>Rubus anatolicus</em> Leaf Extract on Glucose Metabolism in HepG2, CRI-D2 and C2C12 Cell Lines</p>

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