Determination of penicillin G, ampicillin, amoxicillin, cloxacillin and cephapirin by high-performance liquid chromatography—electrospray mass spectrometry

Straub, Rolf; Voyksner, Robert D.

doi:10.1016/0021-9673(93)83336-q

Cited by 104 publications

(69 citation statements)

References 31 publications

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“…It has been reported that fragmental ions of PEN G were m/z 160 and 176 in the positive ion mode (Riediker and Stadler, 2001), and m/z 192 and 289 in the negative mode (Becker et al, 2004) using LC-ESI/MS/MS. In this study, fragmental ions were found to be m/z 192 and 289 for PEN G, the same as that reported using LC-ESI/MS (Straub and Voyksner, 1993). The sensitivity at 20 V was about 6 times higher than that at 60 V, considering the maximum structural information obtained at 60 V for most of the analytes; all the water samples were subsequently analyzed in multi-channel mode, with quantification performed at 20 V using [M-H] À ions and qualitative analysis performed simultaneously at 60 V for all the analytes.…”

Section: Qualification and Quantificationsupporting

confidence: 86%

Determination of penicillin G and its degradation products in a penicillin production wastewater treatment plant and the receiving river

et al. 2008

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a b s t r a c tTo investigate the fate of penicillin G (PEN G) in the wastewater from a PEN G production facility and the receiving river, an analytical method was developed for the simultaneous detection of PEN G and five degradation products using liquid chromatography-electros- were found to be penilloic acid, penicilloic acid and isopenillic acid, which occupied 65.8%, 20.4% and 12.9%, respectively, of the total concentration at the last site. This is the first study on the behaviors of PEN G and its main degradation products in wastewater treatment processes and the aquatic environment.

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Section: Qualification and Quantificationsupporting

confidence: 86%

Determination of penicillin G and its degradation products in a penicillin production wastewater treatment plant and the receiving river

et al. 2008

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“…15 Another e †ective technique is atmospheric pressure ionization, used for investigating drug residues in biological matrices. 16 Recently, electrospray ionization (ESI)17 linked with multiple tandem mass spectrometry (MSn)18 has been employed in the structural characterization of a series of pharmaceutically important penicillin sodium salts. 19 In these conditions, it was observed that [M [ Na]ã nions were the sole peaks present in the spectra, with an abundance two or three orders of magnitude higher than that of the [M ] Na]`positive ion.…”

Section: Introductionmentioning

confidence: 99%

Electrospray mass spectrometry in the structural characterization of cephalosporins

Tenconi¹,

Filippo²,

Col³

et al. 1999

J. Mass Spectrom.

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“…19 However, other studies have shown that a high organic component in the mobile phase yields an optimum response. 20 FddA and F-ddI are not highly retained on reversed-phase columns, so it is difficult to maintain a high organic component and still preserve adequate separation between the salts eluting at the solvent front and the compounds of interest. Therefore, the most retentive column of several tested, Betasil C 18 , was selected.…”

Section: Lc/esi-ms/ms Conditionsmentioning

confidence: 99%

Determination of lodenosine and its major metabolite in human plasma by liquid chromatography/electrospray ionization tandem mass spectrometry

2000

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A sensitive and selective method for the determination of 2'-beta-fluoro-2',3'-dideoxyadenosine (lodenosine, F-ddA), an experimental anti-AIDS drug, and its major metabolite, 2'-beta-fluoro-2',3'-dideoxyinosine (F-ddI), in human plasma was developed and validated. The procedure employs two internal standards and a simple ultrafiltration step followed by chromatography on a Betasil C(18) minibore column. An in-line valve is used to remove salts before reaching the ion source. Detection is by electrospray ionization tandem mass spectrometry with selected reaction monitoring. The method has a limit of quantitation of 4 ng ml(-1) (16 nM) for F-ddA and 8 ng ml(-1) (32 nM) for F-ddI with a linear range up to 2000 ng ml(-1) (7.9 microM) for each. Predicted concentrations from a three-day validation study were within 5% of the nominal values for F-ddA and 16% for F-ddI. Intra- and inter-assay precision, as measured by relative standard deviation, was 13% or better for both compounds. To achieve good reproducibility, many variables related to the electrospray ionization were optimized for both precision and sensitivity. The method was successfully employed to analyze samples and evaluate plasma pharmacokinetics from a Phase I clinical trial.

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Determination of penicillin G, ampicillin, amoxicillin, cloxacillin and cephapirin by high-performance liquid chromatography—electrospray mass spectrometry

Cited by 104 publications

References 31 publications

Determination of penicillin G and its degradation products in a penicillin production wastewater treatment plant and the receiving river

Determination of penicillin G and its degradation products in a penicillin production wastewater treatment plant and the receiving river

Electrospray mass spectrometry in the structural characterization of cephalosporins

Determination of lodenosine and its major metabolite in human plasma by liquid chromatography/electrospray ionization tandem mass spectrometry

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