Determination of PDCD5 in peripheral blood serum of cancer patients

Abstract

Objective: Programmed cell death 5 (PDCD5) is an apoptosis related gene and plays an important role in the pathogenesis and development of cancer. Whether PDCD5 is present in peripheral blood serum has not been reported. The aim of this study is to determine the contents of PDCD5 protein in peripheral blood serum of cancer patients, as well as normal subjects.Methods: ELISA was used to detect the serum PDCD5 concentrations in 100 normal persons, 83 patients with breast cancer, 74 patients with gastrointestinal tract cancer and 41 patients with lung cancer. The results were statistically analyzed and discussed.Results: PDCD5 could be detected in peripheral blood serum in both normal subjects and cancer patients. The serum PDCD5 contents in normal persons ranged from 3.8 to 6.1 ng/ml with a median of 4.70±0.68 ng/ml. For cancer patients the PDCD5 levels were 4.59±0.90, 4.79±1.14 and 10.43±22.34 ng/ml for breast cancer, gastrointestinal cancer and lung cancer patients respectively. There was no statistically significant difference between the serum PDCD5 concentrations of normal persons and cancer patients.Conclusion: PDCD5 is present in peripheral blood. The PDCD5 levels in cancer patients are not statistically different from that of normal persons, though decreased expression of PDCD5 in malignant tissues has been found.

PDCD5 expression predicts a favorable outcome in patients with hepatocellular carcinoma

“…A strong correlation between PDCD5 and IL-17 levels in serum of RA patients has been determined in a previous study (25). Wang et al (13) found decreased expression of PDCD5 in peripheral blood of breast cancer, lung cancer and gastrointestinal cancer, however, the PDCD5 levels in cancer patients are not statistically different from that of normal persons. The level of PDCD5 in serum may be used as a novel marker for HCC.…”

“…Wang et al (12) illustrated a significant elevation of PDCD5 concentrations in both plasma and synovial fluid of knee rheumatoid arthritis (RA) patients compared to osteoarthritis (OA) patients and the control plasma concentrations. The serum PDCD5 levels in breast cancer, gastrointestinal tract cancer and lung cancer patients were separately compared with healthy persons by Wang et al (13). However, the significance of serum PDCD5 and its changes in various cancers await further investigations.…”

PDCD5 regulates cell proliferation, cell cycle progression and apoptosi

“…More recently, it was reported that PDCD5 also regulates autophagy to protect against cardiac remodeling (12). Dysregulation of PDCD5 has been found to be involved in different type of tumors (13)(14)(15)(16)(17)(18)(19)(20)(21)(22). The antitumor activity of PDCD5 has been also proposed (23)(24)(25)(26)(27)(28)(29) and low expression level of PDCD5 has been suggested to be a prognostic indicator for cancers (30).…”

Recombinant human PDCD5 exhibits an antitumor role in hepatocellular carcinoma cells via clathrin-dependent endocytosis

“…PDCD5 is an apoptosis-related gene cloned from TF-1 cells undergoing cytokine deprivation-induced apoptosis (3). In a study by Wang et al (12), no statistically significant difference was observed between the serum PDCD5 concentrations in healthy patients and the patients with breast cancer, gastrointestinal cancer or lung cancer. In this study, it was demonstrated that preoperative serum levels of PDCD5 protein in the patients with HCC were significantly lower than the postoperative serum levels.…”